产品信息

The Monarch Mag Viral DNA/RNA Extraction Kit provides a rapid and reliable magnetic bead-based process for extracting viral nucleic acids from saliva and respiratory swab samples. The kit combines the efficiency of silica-based nucleic acid purification with the ease of use of magnetic beads. Manual and automated workflows allow samples to be processed in microfuge tubes or 96-well plates. Kit sizes align to 96-well formats (100 preps, 600 preps, and 1800 preps), and the protocol is compatible with high throughput automation on a variety of platforms, including the KingFisher Flex magnetic particle processor and Agilent Bravo and MGISP liquid handler platforms.

Properties
Purification format	Magnetic bead
Processing format	Manual or automated
Sample purification (representative examples)	Viral DNA and RNA* from respiratory viruses (enveloped and non-enveloped, dsDNA and ssRNA)
Sample sources	Saliva, respiratory swab in viral transport media (VTM)**
Sample input volume	Up to 200 μl**
Carrier supplier	Poly A carrier RNA***
Binding capacity	Up to 3 μg
Elution volume	33–100 μl
Tested automation platforms	KingFisher Flex; Agilent Bravo and MGISP liquid handlers
Compatible downstream applications	qPCR, RT-qPCR, ddPCR, library prep for NGS

* Viral DNA and RNA are purified in parallel. Preparation of DNA-free RNA or RNA-free DNA requires further treatment with the appropriate nuclease (not supplied).
** The sample input volume may be scalable to accommodate larger sample volumes. Further workflow optimization may be required.
*** Use of carrier RNA is recommended for recovery of low amounts of viral nucleic acid. Carrier RNA should be omitted if the downstream application utilizes poly(A) RNA enrichment; however, viral nucleic acid recovery may be reduced.

 

Figure 1: Monarch Mag Viral DNA/RNA Extraction Kit Workflow

 
Silica-coated Monarch Mag Beads M1 are utilized for highly sensitive nucleic acid capture from samples containing few target molecules. Silanol groups on the bead shell provide an optimal binding surface for nucleic acids, and the uniform, submicron bead size offers a high surface area with abundant binding sites. Beads are supplied as a monodispersed suspension. The superparamagnetic properties of the bead core result in a fast magnetic response, contributing to ease of handling during use and compatibility with automation

An optimized viral nucleic acid extraction procedure employs a sample lysis step followed by a simple bind-wash-elute process. Samples are treated with Proteinase K and then mixed with a lysis buffer/bead mixture containing lysis buffer, carrier RNA, isopropanol, and magnetic beads, which promote binding of viral nucleic acid onto the silica-coated beads. Carrier RNA is utilized as a co-precipitant in the workflow to enhance the recovery of low amounts of viral nucleic acid. After binding of viral DNA and RNA to the magnetic beads, the beads are washed to remove contaminants, and nucleic acid is eluted in nuclease-free water. Purified viral nucleic acid is suitable for downstream applications, including qPCR/RT-qPCR, ddPCR, and library prep for Next Generation Sequencing (NGS).

Figure 2: Complete SARS-CoV-2 genome coverage from wastewater samples using the Monarch Mag Viral DNA/RNA Extraction Kit

Figure 3: Performance comparison of Monarch Mag Viral DNA/RNA Extraction Kit with other suppliers demonstrates high reproducibility and sensitivity of the Monarch kit

Figure 4: qRT-PCR on DNA extracted using the Monarch Mag Viral DNA/RNA Extraction Kit demonstrates accurate detection and quantification of target DNA viruses

Figure 5: RT-qPCR on RNA extracted using the Monarch® Mag Viral DNA/RNA Extraction Kit demonstrates detection of multiple viruses from the same sample

产品类别:

Nucleic Acid Purification Products

应用:

Nucleic Acid Purification

• 试剂盒组成

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  T4010S     25       Monarch® Mag Beads M1 T4005-21 25 1 x 2.4 ml Not Applicable   Monarch® Carrier RNA T4006-21 25 1 x 125 µg Not Applicable   Monarch StabiLyse™ DNA/RNA Buffer T2111-1 25 1 x 24 ml Not Applicable   Monarch® Buffer BX T2041-1 25 1 x 19 ml Not Applicable   Nuclease-free Water B1500-2 25 1 x 25 ml Not Applicable   Proteinase K, Molecular Biology Grade P8200AAVIAL -20 1 x 0.55 ml 20 mg/ml
  T4010L     25       Monarch® Mag Beads M1 T4005-1 25 1 x 14 ml Not Applicable   Monarch® Carrier RNA T4006-1 25 1 x 750 µg Not Applicable   Monarch StabiLyse™ DNA/RNA Buffer T2111-3 25 1 x 145 ml Not Applicable   Monarch® Buffer BX T2041-3 25 1 x 122 ml Not Applicable   Nuclease-free Water B1500-3 25 1 x 122 ml Not Applicable   Proteinase K, Molecular Biology Grade P8200SVIAL -20 3 x 1 ml 20 mg/ml   Proteinase K, Molecular Biology Grade P8200AAVIAL -20 1 x 0.55 ml 20 mg/ml
  T4010X     25       Monarch® Mag Viral DNA/RNA Extraction Kit T4010L 25       Monarch® Mag Beads M1 T4005-1 25 3 x 14 ml Not Applicable   Monarch® Carrier RNA T4006-1 25 3 x 750 µg Not Applicable   Monarch StabiLyse™ DNA/RNA Buffer T2111-3 25 3 x 145 ml Not Applicable   Monarch® Buffer BX T2041-3 25 3 x 122 ml Not Applicable   Nuclease-free Water B1500-3 25 3 x 122 ml Not Applicable   Proteinase K, Molecular Biology Grade P8200SVIAL -20 9 x 1 ml 20 mg/ml   Proteinase K, Molecular Biology Grade P8200AAVIAL -20 3 x 0.55 ml 20 mg/ml

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操作说明、说明书 & 用法

• 操作说明

  1. Reagent Preparation Guidance
  2. Protocol for Manual Isolation of Viral DNA/RNA in Microfuge Tubes (1.5 or 2.0 ml)
  3. Protocol Guidance for Automated Isolation of Viral DNA/RNA in Deep Well Plates
  4. Protocol for KingFisher Flex Automated Isolation of Viral DNA/RNA

• 说明书

  产品说明书包含产品使用的详细信息、产品配方和质控分析。

  • manualT4010

FAQs & 问题解决指南

• FAQs

  1. The enzymes in the kit were not stored at -20°C right away. Will they still work?
  2. How do I prepare Viral DNA/RNA Wash Buffer?
  3. Does the kit include Nuclease-Free Water to prepare all wash buffers?
  4. Where can I find automation scripts?
  5. What is the sample input volume range?
  6. What sample types are compatible with T4010?
  7. What downstream applications are compatible with T4010?
  8. What is the purpose of Carrier RNA, and is it necessary to include in the extraction?