标签归档：trypsin

Trypsin-digested BSA MS Standard (CAM-modified) |NEB酶试剂 New England Biolabs

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产品信息

A complex mixture of peptides produced by Trypsin digestion of Bovine Serum Albumin (BSA) that was reduced and alkylated with Iodoacetamide (CAM modified). This peptide mixture can be used to test a Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF) or Electrospray Ionization (ESI) mass spectrometer (TOF, Q-TOF or Ion Trap).

MALDI Analysis of BSA Digest:
Trypsin-digested BSA MS Standard (CAM-modified) |
The BSA digest is dissolved to 1 pmol/μl. One µl of digest is mixed with 1 µl of α-cyano-4-hydroxycinnamic acid (10 mg/ml in 50:50 acetonitrile:water with 0.1% trifluoroacetic acid). One μl of the digest/matrix solution were then spotted directly onto a MALDI target plate. 
Online Analysis of BSA Digest:
Trypsin-digested BSA MS Standard (CAM-modified) |
The BSA digest solution was diluted to 100 fmol/μl with 0.1% formic acid. One μl (100 fmol) of the digest solution was injected via an Easy-nanoLC II (Proxeon) onto a self-packed reverse phase C18 nano column (Phenomenex packing material, 5 cm, 150 μm) and loaded onto a self-packed C18 analytical column with an integrated tip (New Objective Picofrit, 15 cm, 100 μm, Phenomenex packing material). Peptides were separated using a 45 min 5-70%B gradient (A = 0.1% formic acid, B = CH3CN, 0.1% formic acid) at a flow rate of 400 nl/min. Eluting peptides were analyzed online by a Q-Exactive mass spectrometer (Thermo Scientific) with an electrospray ion source. MS/MS spectra were acquired by HCD using an acquisition range of 400 to 1600 m/z.

产品来源

BSA (GENBANK P02769) was digested using Trypsin (TPCK-treated).

重组

Suggested volume to resuspend: 500 µl. Avoid repeated freeze/thaw cycles once in solution.

产品类别:
Proteome Analysis Products

应用:
Proteomics

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • P8108S     -20    
        Trypsin-digested BSA MS Standard (CAM-modified) P8108SVIAL -20 1 x 500 pmol Not Applicable

  • 注意事项

    1. Supplied in dry format.
    2. This product has lot-to-lot variations in peptide frequency and abundance.

rLys-C + Trypsin 重组赖氨酰肽链内切酶/重组胰蛋白酶混合装

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rLys-C + Trypsin

重组赖氨酰肽链内切酶/重组胰蛋白酶混合装

品牌：IMD
CAS No.：
储存条件：-15℃以下
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

IMD-RME-C020X5

 for Proteome Research 20 ug×5 – 咨询 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

rLys-C + Trypsin 重组赖氨酰肽链内切酶/重组胰蛋白酶混合装

发表于由

rLys-C + Trypsin

重组赖氨酰肽链内切酶/重组胰蛋白酶混合装

品牌：IMD
CAS No.：
储存条件：-15℃以下
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

IMD-RME-C020

 for Proteome Research 20 ug – 咨询 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

MonoSpin Trypsin

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MonoSpin Trypsin

品牌：Funakoshi
CAS No.：
储存条件：室温
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

782011300

 – 50 pieces – 咨询 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。