Sigma 放线菌素 D

产品编号 A9415 | CAS号 50-76-0 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.
Application
Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation with wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells, and as an antibiotic in treatment of various malignant neoplasms, including Wilm′s tumour and the sarcomas. Recommended for us in cell culture applications at 1μg/mL.
Caution
This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.
包装：2, 5, 10, 25 mg in glass bottle
Preparation Note
Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.
Biochem/physiol Actions
Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Sigma 二甲基亚砜DMSO

产品编号 D2650 | CAS号 67-68-5 | SIGMA
Frequently Asked Questions
Frequently Asked Questions are available for this Product.
Application
DMSO 是一种用于化学反应（如聚合酶链式反应 (PCR)）的极性非质子溶剂，也可作为冷冻保护玻璃化剂用于保存细胞、组织和器官。DMSO 可在细胞冷冻培养基中用于保护细胞免受冰晶导致的机械损伤。它可用于原代、继代培养和重组的异倍体和杂交瘤细胞系、胚胎干细胞 (ESC) 以及造血干细胞的冻存。DMSO 常与 BSA 或胎牛血清 (FBS) 一同使用。
Caution
容易过度冷却，并在室温下缓慢融化固化的产品放在室温下可以再次液化，对其本身无影响。
Other Notes
This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.
包装:5mL 和 10mL 火焰封口安瓿瓶包装，100mL 棕色瓶包装

sigma 醋氨酚BioXtra

产品编号 A7085 | CAS号 103-90-2 | SIGMA-ALDRICH

Application：镇痛剂。

包装：1 kg in glass bottle，100, 500 g in glass bottle

sigma-白蛋白 来源于牛血清

产品编号 A3803 | CAS号 9048-46-8 | SIGMA

包装：10, 50, 100 g in poly bottle

Preparation Note
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn^[] method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term “Fraction V” has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
采用热休克分级分离法制备。经过进一步纯化以降低脂肪酸含量。

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

 
产品编号 A8806 | CAS号 9048-46-8 | SIGMA

包装：1, 5 g in poly bottle

Preparation Note
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn^[] method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term “Fraction V” has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
通常称为 Cohn fraction V；本品采用改进的 Cohn 氏冷乙醇分级分离法制备。

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.^[]
Protocols & Applications
Select the right albumin for your application with the Albumin Selection Guide