产品信息

The Color Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, and two reference bands (one orange and one green at 72 kDa and 26 kDa, respectively), that resolves into 11 sharp bands when electrophoresed. The Color Prestained Protein Standard is designed for observing protein separation during SDS-PAGE, verification of western transfer efficiency on membranes, and for approximating the size of proteins (1-2). The Color Prestained Protein Standard may also be used in fluorescent imaging of SDS-PAGE.

产品类别:

Protein Standards Products,

Markers & Ladders Products,

Protein Standards Products

应用:

Protein Standards,

Protein Analysis Tools

• 产品组分信息

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  P7719S     -20       Color Prestained Protein Standard, Broad Range (10-250 kDa) P7719SVIAL -20 1 x 150 gel lanes Not Applicable
  P7719L     -20       Color Prestained Protein Standard, Broad Range (10-250 kDa) P7719SVIAL -20 5 x 150 gel lanes Not Applicable

• 特性和用法

  贮存溶液

  62.5 mM Tris-phosphate
  1 mM EDTA
  2% SDS
  10 mM DTT
  1 mM NaN₃
  33% Glycerol
  pH 7.5 @ -20°C

• 优势和特性

  Features

  • Direct loading, additional loading buffer and heat incubation not required
  • Applications include verification of western blot transfer efficiency on membranes and fluorescent imaging of SDS-PAGE

• 相关产品

  相关产品

  • Blue Prestained Protein Standard, Broad Range (11-250 kDa)
  • Unstained Protein Standard, Broad Range (10-200 kDa)

• 注意事项

  1. To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells.
  2. To maximize shelf life, store at -20°C. Guaranteed stable for 2 years when properly stored.
  3. It is not necessary to pre-heat the unstained protein standard prior to loading on a gel.
  4. Recommended mini-gel load: 3 μl.
  5. Apparent molecular weight values for prestained proteins can be different when run on different gel types and percentages.

• 参考文献

  1. Laemmli, UK (1970). Nature. 227, 680.
  2. Sambrook, J., Fitsch, EF. and Maniatis, T. (2001). Molecular Cloning: A Laboratory Manual. (3rd ed.). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

操作说明、说明书 & 用法

• 操作说明

  1. Protocol for Using the Color Prestained Protein Standard, Broad Range (10-250 kDa) (NEB #P7719) for an SDS-PAGE Mini Gel

FAQs & 问题解决指南

• FAQs

  1. How does the new Color Prestained Protein Standard, Broad Range differ from NEB’s previous Color Prestained Protein Standard?
  2. What is the protein concentration of the proteins used in the Color Prestained Protein Standard, Broad Range?
  3. I accidentally left my Protein Standard out at room temperature for a number of days. Is it still okay to use?
  4. I notice that the molecular weights I am observing are slightly different than those reported. Can you explain?
  5. What volume is supplied in a small pack and a large pack of NEB’s Protein Standards?