单位定义
One unit is defined as the amount of enzyme required to incorporate 1 nmol of ATP into acid-insoluble material in 1 hour at 50°C.
Reaction Conditions
1X Hi-T7 RNA Polymerase Reaction Buffer, supplemented with 0.5 mM each of ATP, UTP, GTP, CTP, and DNA template containing the T7 RNA Polymerase promoter. Incubate at 37-56°C for 1 hour. Optimal incubation temperature is 50-52°C.
反应条件
1X Hi-T7® RNA Polymerase Reaction Buffer
Supplement with 0.5 mM ATP, 0.5 mM UTP , 0.5 mM GTP 和 0.5 mM CTP
Incubate at 50°C
1X Hi-T7® RNA Polymerase Reaction Buffer
40 mM Tris-HCl
4 mM MgCl2
50 mM NaCl
2 mM spermidine
1 mM DTT
(pH 7.5 @ 25°C)
贮存溶液
50 mM Tris-HCl
100 mM NaCl
1 mM EDTA
1 mM DTT
0.1% (w/v) Triton® X-100
50% Glycerol
pH 7.9 @ 25°C
单位活性检测条件
1X Hi-T7 RNA Polymerase Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP and 1 µg T7 phage DNA in 50 µl reaction volume.