标签归档：nuclei

Monarch® gDNA Nuclei Prep & Lysis Buffer Pack |NEB酶试剂 New England Biolabs

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产品信息

The Monarch gDNA Nuclei Prep & Lysis Buffer Pack contains two lysis buffers used in the Monarch HMW DNA Extraction Kit for Cells & Blood (NEB #T3050). The Nuclei Prep Buffer is a mild lysis buffer used first to lyse the cell membrane and release the contents of the cytoplasm while the nuclei are kept intact. It is used pre-mixed with the RNase A enzyme included in the kit. After the RNA from the cytoplasm is digested by RNase A, the Nuclei Lysis Buffer, pre-mixed with the included Proteinase K, is used to lyse the nuclei and release the HMW genomic DNA. This two-step lysis ensures that RNA in the cytoplasm is effectively degraded before the viscous genomic DNA from the nuclei is released. 20 ml of each buffer is included in this buffer pack. The Nuclei Prep Buffer is used chilled to 4°C, and it can be stored at 4°C for convenience if desired.

产品类别:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T3054L     25    
        Monarch® gDNA Nuclei Lysis Buffer T3053-1 25 2 x 10 ml Not Applicable
        Monarch® gDNA Nuclei Prep Buffer T3052-1 25 2 x 10 ml Not Applicable

  • 相关产品

    相关产品

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    • Monarch 捕获珠分离管
    • Monarch® 收集管 II
    • p8107-proteinase-k-molecular-biology-grade
    • Monarch 2 ml 离心管
    • T3015 Monarch gDNA Wash Buffer

操作说明、说明书 & 用法

  • 操作说明

    1. Download Quick Protocol Card for Cells
    2. Download Quick Protocol Card for Blood
    3. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Cells (NEB #T3050)
    4. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Blood (NEB #T3050)
    5. Protocol for UHMW DNA Cleanup in the Oxford Nanopore Technologies® UL Library Prep Workflow

FAQs & 问题解决指南

  • 问题解决指南

    • Troubleshooting Guide for Monarch HMW DNA Extraction Kits (NEB #T3050 and #T3060)