标签归档：ni

Nickel Standard Solution (Ni 1000) 镍标准溶液（Ni 1000）

发表于由

品牌：FUJIFILM Wako
CAS No.：
储存条件：25℃以下
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

141-09781

 for ICP Analysis 100mL – 咨询 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

产品描述相关资料下载免责声明相关产品浏览记录 请联系客服

1. 本公司密切关注本网站发布的内容，但不保证发布内容的准确性、完整性、可靠性和最新性等。

2. 本公司不保证使用本网站期间不会出现故障或计算机病毒污染的风险。

3. 无论何种原因，使用本网站时给用户或第三方造成的任何不利或损害，本公司概不负责。此外，对于用户与其他用户或第三方之间因本网站发生的任何交易、通讯或纠纷，本

3. 公司概不负责。

4. 本网站可提供的所有产品和服务均不得用于人体或动物的临床诊断或治疗，仅可用于科研等非医疗目的。如任何用户将本网站提供的产品和服务用于临床诊断或治疗，以及其

4. 他特定的用途或行为，本公司概不保证其安全性和有效性，并且不负任何相关的法律责任。

Express Ni Spin Columns |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

Spin columns containing an affinity matrix for the small-scale isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. Immobilized Metal Affinity Chromatography (IMAC) purifications employing NEBExpress® Ni Spin columns can be performed under native or denaturing conditions, yielding highly pure target in a single protein purification step.  This enables screening of expression conditions and streamlines the functional and structural characterization of the target protein.

Support Matrix: Spherical, agarose based microparticles ranging in size from 10-100 μm.
 

Figure 1: NEBExpress Ni Spin Column Quick Start Protocol

Express Ni Spin Columns |

Figure 2: Purification of His-tagged fusion proteins using NEBExpress Ni Spin Columns

Express Ni Spin Columns |

NEBExpress Ni Spin Columns were equilibrated and loaded with 500 μl of crude extract from E. coli containing a plasmid that expresses either His6-GluRS or His6-NDK fusion protein. Columns were washed, eluted and analyzed by SDS-PAGE to confirm low non-specific binding of extract proteins and high iso­lation of target. Marker (M) contains Color Prestained Protein Standard (NEB #P7719), Lane 1: GluRS protein load, Lane 2: GluRS flow through, Lane 3: GluRS 1st elution, Lane 4: GluRS 2nd elution, Lane 5: NDK protein Load, Lane 6: NDK flow through, Lane 7: NDK 1st elution, Lane 8: NDK 2nd elution.
产品类别:
Amylose Purification (MBP-tag),
Nickel Purification (His-tag),
Affinity Purification Products,

Protein Purification Products

应用:
MBP Affinity Tag,
Fusion Protein Cleavage,
Pull Down Assays,

His-tagged Protein Expression & Purification,
Affinity Purification & Expression Tags,
Protein Purification,

Protein Analysis Tools

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • S1427S     4    
        NEBExpress® Ni Spin Columns S1427SVIAL 4 10 x 1 Each
      (0.1 ml)       		Not Applicable
        2X IMAC Buffer B1076SVIAL 4 1 x 30 ml 2 X
        2M Imidazole B1077SVIAL 4 1 x 2 ml 2 M
    • S1427L     4    
        NEBExpress® Ni Spin Columns S1427SVIAL 4 25 x 1 Each
      (0.1 ml)       		Not Applicable
        2X IMAC Buffer B1076SVIAL 4 2 x 30 ml 2 X
        2M Imidazole B1077LVIAL 4 1 x 10 ml 2 M

  • 特性和用法

    贮存溶液

    20% Ethanol

    支持介质

    Spherical, agarose based microparticles ranging in size from 10-100 μm.

    结合容量

    Varies with target, ≥ 1 mg His-tagged fusion protein per column.

  • 优势和特性

    Features

    • Isolation and purification of His-tagged fusion proteins under native or denaturing conditions as IMAC tolerates a wide range of conditions, including the presence of protein denaturants, reducing agents and detergents.
    • High specific binding of His-tagged proteins from various expression systems yielding milligram quantities of target protein with purities of >95%.
    • Can be used with common cell lysis reagents and a variety of buffer additives

  • 相关产品

    相关产品

    • NEBExpress® Ni-NTA 磁珠
    • TEV Protease
    • Amylose Resin
    • Amylose Resin High Flow
    • 几丁质树脂
    • NEBExpress® Ni Resin

  • 注意事项

    1. Do not freeze.
    2. Several E. coli proteins are capable of binding immobilized metal affinity matrices with moderate to high affinity. The most common contaminants include SlyD (28kDa), GlmS (67kDa), ArnA (74kDa) and carbonic anhydrase (25 kDa) (1). The named contaminants may be eliminated from the Ni column elution fraction by employing NiCo21(DE3) (NEB# C2529H) as the expression host and then performing a second binding step with chitin magnetic beads (NEB# E8036) (2). 
    3. Protein yield and purity are dependent upon the expression level, conformation and solubility characteristics of the recombinant fusion protein.  For best results, estimate the expression level of the His-tagged protein of interest by first running a sample of the crude lysate on an SDS-PAGE gel. 
    4. Unlike most IMAC resins, the unique chemistry employed in NEBExpress™ Ni Resin provides chemical tolerance to chelating agents such as EDTA and reducing agents such as DTT.  Please consult the chemical compatibility table for more information. 
    5. Polyhistidine tags do not typically compromise the biological protein function and are not considered immunogenic, however if cleavage of the His-tag is necessary we recommend using TEV Protease (NEB# P8112).
    6. Ni resin yields highly variable binding capacities dependent on the target and conditions. The binding capacity value (≥ 1 mg/column) for this product was determined by performing a mock purification from crude cell lysates expressing a His-tagged target protein. 

  • 参考文献

    1. Bolanos-Garcia et al (2006). BBA. 1760, 1304-1313.
    2. Samuelson, J (2016). GEN. 26-27.

操作说明、说明书 & 用法

  • 操作说明

    1. NEBExpress® Ni Spin Columns Quick Start Protocol (NEB #S1427)
    2. NEBExpress® Ni Spin Column Reaction Protocol (NEB #S1427)
    3. His-tag removal from protein using TEV Protease

  • 应用实例

    • NEBExpress® Cell-free E. coli Protein Synthesis System

工具 & 资源

  • 选择指南

    • Purification Beads, Columns and Resins

FAQs & 问题解决指南

  • FAQs

    1. How much lysate can be loaded onto a single NEBExpress Ni Spin Column?
    2. Will extended binding increase the yield of the target protein in the eluate?
    3. What is the minimum recommended load volume?
    4. Is it necessary to cap the column during each centrifugation step?
    5. Why is the liquid not completely removed during the centrifugation step?
    6. I’ve prepared my lysate with too much buffer and the target is very dilute, can I apply more lysate by repeating the load steps?
    7. How can I reduce contaminating proteins in a Ni spin column protein purification?
    8. What are the recommendations for pH based elution, as opposed to imidazole?
    9. How can I remove imidazole from a protein sample?
    10. What are to the advantages of performing purification under denaturing conditions?
    11. How can I determine if my target protein remains bound to the resin?
    12. Why is imidazole not necessary in the lysis/binding buffer? Why does the wash buffer contain only 5mM imidazole?

  • 问题解决指南

    • Troubleshooting Guide for Purification using NEBExpress® Ni Spin Columns

Express Ni-NTA 磁珠 |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

一种用于手动或自动少量分离和纯化多组氨酸标签（His 标签）融合蛋白的亲和介质。使用 Ni-NTA（镍-次氨基三乙酸）磁珠用于固定化金属亲和色谱法（IMAC）纯化蛋白时，无论是未变性或变性条件下，均可有效结合并纯化不溶性蛋白质、聚集在包涵体中的蛋白质、或具有隔离聚组氨酸亲和标记的三级结构的蛋白。被磁珠结合的 His 标签蛋白质可用于 pull-down 实验中捕获与其相互作用的蛋白质。

如有更大量的需求，可通过 NEB 的 OEM/Bulks 部门购买定制大包装产品。更多信息请联系 [email protected]。

产品类别:
Nickel Purification (His-tag),
Affinity Purification Products,
Magnetic Beads and Racks Products,

Protein Purification Products

应用:
MBP Affinity Tag,
Fusion Protein Cleavage,
Pull Down Assays,

His-tagged Protein Expression & Purification,
Affinity Purification & Expression Tags,
Protein Purification,
Protein Analysis Tools,

High-throughput cloning and automation solutions

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • S1423S     4    
        NEBExpress® Ni-NTA Magnetic Beads S1423SVIAL 4 1 x 1 ml 10 %
        2X IMAC Buffer B1076SVIAL 4 1 x 30 ml 2 X
        2M Imidazole B1077SVIAL 4 1 x 2 ml 2 M
    • S1423L     4    
        NEBExpress® Ni-NTA Magnetic Beads S1423SVIAL 4 5 x 1 ml 10 %
        2X IMAC Buffer B1076SVIAL 4 5 x 30 ml 2 X
        2M Imidazole B1077LVIAL 4 1 x 10 ml 2 M

  • 特性和用法

    贮存溶液

    20% Ethanol

    支持介质

    大小在 20-100 μm 的琼脂糖基超顺磁球形微粒。

    结合容量

    因纯化靶标不同而异，通常 1 ml 柱床体积结合 ≥ 7.5 mg His 标签融合蛋白。

  • 优势和特性

    Features

    • 在未变性或者变性条件下分离及纯化 His 标签融合蛋白，因为 IMAC 兼容各种条件，包括含有蛋白变性剂和洗涤剂时。
    • 具有高特异性 His 标签蛋白结合能力，可与多种表达体系结合且纯度 > 95%。
    • NTA 通过四个配位点牢固地结合金属离子，从而减少镍离子浸出。
    • 可与常用的细胞裂解试剂和多种缓冲液添加剂一起使用。

  • 相关产品

    相关产品

    • TEV Protease
    • 6 孔磁性分离架
    • 12 孔磁性分离架
    • 96 孔微孔板磁性分离架
    • 50 ml 磁性分离架

  • 注意事项

    1. 请勿冷冻。
    2. 请勿贮存在含咪唑的缓冲液中。
    3. 多种大肠杆菌蛋白均能结合固定化金属亲和基质，亲和性由中到高。最常见的污染物包括 SlyD（28kDa）、GlmS（67kDa）、ArnA（74kDa）和碳酸酐酶（25 kDa）（1）。可以将 NiCo21（DE3）（NEB# C2529H）作为表达宿主，然后使用几丁质磁珠（NEB# E8036）（2）来进行第二步的结合，以去除 Ni-NTA 洗脱组分中的上述污染物。
    4. 蛋白产量和纯度取决于重组融合蛋白的表达水平、构象和溶解度特征。为了获得最佳结果，请先进行小量试验以估计每种 His 标签蛋白的表达水平并确定其溶解度。
    5. 请避免使用蛋白酶抑制剂或其它含螯合剂（如 EDTA）或强还原剂（如 DTT 或 β-巯基乙醇）的添加剂，它们会影响 NEBExpress™ Ni-NTA 磁珠的性能。
    6. 多组氨酸标签一般不会影响生物蛋白功能，或引起免疫反应，但如需切割 His 标签，推荐使用 TEV 蛋白酶（NEB# P8112）。
    7. NEBExpress™ NEBExpress Ni-NTA 磁珠的结合能力会因靶标和反应条件的不同而有很大差异。此产品的结合能力（≥ 7.5 mg/ml）通过从添加有 his 标签目的蛋白的粗细胞裂解物中进行模拟纯化确定。据报道，大多数商业化 IMAC 吸附剂的结合能力高达 40 mg/mL。然而，这些值指的是分离出的纯蛋白或合成混合物中的结合能力，对于从复杂来源中分离目的蛋白的结合能力一般较低。

  • 参考文献

    1. Bolanos-Garcia et al (2006). BBA. 1760, 1304-1313.
    2. Samuelson, J (2016). Purifying Recombinant His-Tagged Proteins. Genetic Engineering & Biotechnology News. 26-27.

操作说明、说明书 & 用法

  • 操作说明

    1. NEBExpress® Ni-NTA Magnetic Beads Typical Reaction Protocol
    2. Quick Start Protocol for NEBExpress® Ni-NTA Magnetic Beads
    3. His-tag removal from protein using TEV Protease

  • 应用实例

    • NEBExpress® Cell-free E. coli Protein Synthesis System

工具 & 资源

  • 选择指南

    • Purification Beads, Columns and Resins

FAQs & 问题解决指南

  • FAQs

    1. Which E. coli strain do you recommend to reduce contaminating proteins?
    2. The recovery of the His-tagged target protein is low relative to contaminant proteins. Should I add more NEBExpress® Ni-NTA Magnetic Beads to improve recovery of my target protein?
    3. How can I reduce contaminating proteins in my Ni-NTA His-tag protein purification?
    4. My purification failed and/or why is my yield lower than expected?
    5. How can I remove imidazole from a protein sample?
    6. What is the minimum elution volume that should be used?
    7. Why is there is so much target protein in the column flow through?
    8. Why is there variability in my high throughput assay?
    9. Should the crude extract be diluted with lysis buffer to increase the volume for the binding step?
    10. What is a typical protocol for lysing cells?
    11. What are the recommendations for pH based elution, as opposed to imidazole?
    12. What are to the advantages of performing purification under denaturing conditions?

Express Ni Resin |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface.  It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and β-Mercaptoethanol.

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.

Support Matrix: Spherical, agarose based microparticles ranging in size from 10-100 μm.
产品类别:
Amylose Purification (MBP-tag),
NEBExpress MBP Fusion and Purification System,
Bacterial E. coli Protein Expression Products,

Nickel Purification (His-tag),
Affinity Purification Products,
Protein Purification Products,

Protein Expression Products

应用:
MBP Affinity Tag,
Fusion Protein Cleavage,
Pull Down Assays,

His-tagged Protein Expression & Purification,
Affinity Purification & Expression Tags,
Protein Purification,

Protein Analysis Tools

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • S1428S     4    
        NEBExpress® Ni Resin S1428SVIAL 4 1 x 25 ml 50 %

  • 特性和用法

    贮存溶液

    20% Ethanol

    支持介质

    Spherical, agarose based microparticles ranging in size from 10-100 μm.

    结合容量

    1 ml of NEBExpress™ Ni Resin will bind ≥ 10 mg of His-tagged fusion protein.

  • 优势和特性

    Features

    • Isolation and purification of His-tagged fusion proteins under native or denaturing conditions as IMAC tolerates a wide range of conditions, including the presence of protein denaturants, reducing agents and detergents.
    • High specific binding of His-tagged proteins from various expression systems yielding milligram quantities of target protein with purities of >95%.
    • Can be used with common cell lysis reagents and a variety of buffer additives

  • 相关产品

    相关产品

    • NEBExpress® Ni-NTA 磁珠
    • TEV Protease
    • Amylose Resin
    • Amylose Resin High Flow
    • 几丁质树脂
    • NEBExpress® Ni Spin Columns

  • 注意事项

    1. Do not freeze.
    2. Several E. coli proteins are capable of binding immobilized metal affinity matrices with moderate to high affinity. The most common contaminants include SlyD (28kDa), GlmS (67kDa), ArnA (74kDa) and carbonic anhydrase (25 kDa) (1). The named contaminants may be eliminated from the Ni column elution fraction by employing NiCo21(DE3) (NEB# C2529H) as the expression host and then performing a second binding step with chitin magnetic beads (NEB# E8036) (2). 
    3. Protein yield and purity are dependent upon the expression level, conformation and solubility characteristics of the recombinant fusion protein.  For best results, estimate the expression level of the His-tagged protein of interest by first running a sample of the crude lysate on an SDS-PAGE gel. 
    4. Unlike most IMAC resins, the unique chemistry employed in NEBExpress™ Ni Resin provides chemical tolerance to chelating agents such as EDTA and reducing agents such as DTT.  Please consult the chemical compatibility table for more information. 
    5. Polyhistidine tags do not typically compromise the biological protein function and are not considered immunogenic, however if cleavage of the His-tag is necessary we recommend using TEV Protease (NEB# P8112).
    6. Ni resin yields highly variable binding capacities dependent on the target and conditions. The binding capacity value (≥ 10 mg/ml) for this product was determined by performing a mock purification from crude cell lysates expressing a His-tagged target protein.

  • 参考文献

    1. Bolanos-Garcia et al (2006). BBA. 1760, 1304-1313.
    2. Samuelson, J (2016). GEN. 26-27.

操作说明、说明书 & 用法

  • 操作说明

    1. NEBExpress® Ni Resin Gravity Flow Typical Protocol (NEB #S1428)
    2. NEBExpress® Ni Resin Batch Binding Typical Protocol (NEB #S1428)
    3. NEBExpress® Ni Resin Pressurized Column Typical Protocol (NEB #S1428)
    4. NEBExpress® Ni Resin Quick Start Protocol (NEB #S1428)
    5. His-tag removal from protein using TEV Protease

工具 & 资源

  • 选择指南

    • Purification Beads, Columns and Resins

FAQs & 问题解决指南

  • FAQs

    1. Can NEBExpress® Ni Resin be used in large scale, pressurized purification systems such as FPLC?
    2. Will extended binding increase the yield of the target protein in the eluate?
    3. Does NEBExpress Ni Resin need to be regenerated with nickel after cleaning?
    4. How can I reduce contaminating proteins in a protein purification when using Ni Resin?
    5. What are the recommendations for pH based elution, as opposed to imidazole?
    6. How can I remove imidazole from a protein sample?
    7. What are to the advantages of performing purification under denaturing conditions?
    8. How can I determine if my target protein remains bound to the resin?
    9. Why is imidazole not necessary in the lysis/binding buffer? Why does the wash buffer contain only 5 mM imidazole?
    10. Why is my protein precipitating on the column?
    11. What gravity column do you recommend?
    12. How can I determine if my target protein remains bound to the resin?

  • 问题解决指南

    • Troubleshooting Guide for Purification using NEBExpress® Ni Resin