上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
产品信息
本酶提供高保真版 MfeI-HF®（NEB #R3589）
高保真（HF）限制性内切酶在 rCutSmart 缓冲液中具有 100% 活性；统一缓冲液意味着更加直接、简化的样品处理过程。HF 内切酶还会显著降低星号活性。所有 HF 内切酶均符合省时酶（Time-Saver）标准，可在 5-15 分钟内酶切底物 DNA，也可实现过夜酶切而不会造成 DNA 降解。HF 限制性内切酶在改造时将性能作为重要指标，可在更宽的条件下具有完全活性，最大限度地减少非特异性酶切产物，并且为实验设计提供灵活性。
产品来源
大肠杆菌菌株，携带有克隆自发酵支原体 Mycoplasma fermentans（N.F. Halden）的 MfeI 基因。
- 产品类别:
- Discontinued (<3 years)
-
特性和用法
单位定义
一个单位是指在 50 µl 的总反应体系中，37℃ 条件下，1 小时内酶切 1 µg λ DNA 所需的酶量。
反应条件
1X rCutSmart™ 缓冲液
Incubate at 37°C1X rCutSmart™ 缓冲液
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)在不同缓冲液中的活性
NEBuffer™ r1.1: 75%
NEBuffer™ r2.1: 50%
NEBuffer™ r3.1: 10%
rCutSmart™ Buffer: 100%稀释兼容性
- 稀释液 A
贮存溶液
10 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C热失活
否
甲基化敏感性
dam 甲基化: 不敏感
dcm 甲基化: 不敏感
CpG甲基化: 不敏感同裂酶
MfeI-HF
MunI -
相关产品
相关产品
- MfeI-HF®
- t1010-monarch-plasmid-miniprep-kit
- Monarch® DNA 胶回收试剂盒
- Monarch® PCR & DNA 纯化试剂盒（5 μg）
- Nuclease-free Water
单独销售的组分
- rCutSmart™ 缓冲液
-
注意事项
- MfeI 是 MunI 的完全同裂酶。
- 对 CpG、dcm 或 dam 甲基化均不敏感。
- 延长酶切时间可能产生星号活性。
操作说明、说明书 & 用法
-
操作说明
- Optimizing Restriction Endonuclease Reactions
- Restriction Digest Protocol
- Double Digest Protocol with Standard Restriction Enzymes
-
使用指南
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Alteration of Apparent Recognition Specificities Using Methylases
- Cleavage Close to the End of DNA Fragments
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Heat Inactivation
- Restriction Endonucleases – Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Single Letter Codes
- Star Activity
工具 & 资源
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选择指南
- Alphabetized List of Recognition Sequences
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Frequencies of Restriction Sites
- Isoelectric Points (pI) for Restriction Enzymes
- Isoschizomers
- NEB Diluent and Buffer Table
- Recleavable Filled-in 5′ Overhangs
- Time-Saver™ Qualified Enzymes
-
Web 工具
- DNA Sequences and Maps Tool
- Double Digest Finder
- Enzyme Finder
- NEBcutter™ v3.0
- NEBioCalculator®
- REBASE®
FAQs & 问题解决指南
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FAQs
- Is MfeI inhibited by salt?
- Does MfeI have trouble cleavin PCR products?
- What is the molecular weight of MfeI?
- Does MfeI have single sites in common vectors?
- What is the activity of MfeI at 25°C?
- I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
- Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
- Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
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