上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
产品信息
The New England Biolabs Magnetic mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells and tissue without requiring phenol or other organic solvents. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour. Oligo d(T)25 Magnetic Beads can be reused up to three times and the researcher has the option of eluting the isolated mRNA or using the bound dT DNA as a primer in a first-strand cDNA reaction. Figure 1: Consistency and wide isolation range are demonstrated by poly(A)+ RNA isolation from duplicate samples of decreasing numbers of HEPG2 cells (5 x 105 to 1 x 103) by direct lysis/binding in microtiter plate followed by mRNA isolation with the magnetic method. 1/10th of isolated mRNA is converted to oligo (dT) primed cDNA using ProtoScript First Strand cDNA Synthesis Kit (NEB #E6500) and qPCR done with validated primers for the peptidylpropyl isomerase, a low-abundance housekeeping gene.
Figure 2: Facilitate the isolation of poly(A)+ RNA using the Magnetic mRNA Isolation kit
产品类别:
Total RNA Extraction & Purification Products,
RNA Extraction and Purification
试剂盒组成
本产品提供以下试剂或组分：
NEB #
名称
组分货号
储存温度
数量
浓度
S1550S
4
Oligo d(T)25 Magnetic Beads
S1419SVIAL
4
1 x 25 mg
5 mg/ml
Lysis/Binding Buffer
B1556AVIAL
4
1 x 40 ml
1 X
Wash Buffer I
B1558AVIAL
4
1 x 40 ml
1 X
Wash Buffer II
B1559AVIAL
4
1 x 40 ml
1 X
Low Salt Buffer
B1557AVIAL
4
1 x 30 ml
1 X
Elution Buffer
B1561AVIAL
4
1 x 5 ml
1 X
优势和特性
Features
Suitable for automated high-throughput applications
Eliminates need for phenol or other organic solvents
No need to precipitate poly(A)+ transcripts in eluent
Obtain intact poly(A)+ RNA in less than one hour
Negligible gDNA contamination
Reusable beads
参考文献
Wu, N. et al. (2004). Mol. Biotech.. 27, 119-125.
操作说明、说明书 & 用法
操作说明
Isolation of mRNA from Mammalian Cells Using the Magnetic mRNA Isolation Kit (NEB #S1550)
Isolation of mRNA from Tissue Using the Magnetic mRNA Isolation Kit (NEB #S1550)
Quantitation of Isolated Poly(A) RNA from the Magnetic mRNA Isolation Kit (NEB #S1550)
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
产品信息
The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from 1-5µg of previously isolated total RNA. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour.
Note that for input amounts >5µg we recommend the NEBNext High Input Poly(A) mRNA Isolation Module (NEB #E3370).
产品类别:
RNA Depletion & mRNA Enrichment Products,
RNA Library Prep for Illumina,
Next Generation Sequencing Library Preparation Products
产品组分信息
本产品提供以下试剂或组分：
NEB #
名称
组分货号
储存温度
数量
浓度
E7490S
4
NEBNext RNA Binding Buffer (2X)
E7492AVIAL
4
1 x 7.2 ml
Not Applicable
NEBNext Wash Buffer
E7493AVIAL
4
1 x 28.8 ml
Not Applicable
Nuclease-free Water
E7495AVIAL
4
1 x 1.2 ml
Not Applicable
NEBNext Tris Buffer
E7496AVIAL
4
1 x 6 ml
Not Applicable
NEBNext Oligo d(T)25 Beads
E7499AVIAL
4
1 x 0.48 ml
Not Applicable
E7490L
4
NEBNext RNA Binding Buffer (2X)
E7492AAVIAL
4
1 x 28.8 ml
Not Applicable
NEBNext Wash Buffer
E7493AAVIAL
4
2 x 57.6 ml
Not Applicable
Nuclease-free Water
E7495AAVIAL
4
1 x 4.8 ml
Not Applicable
NEBNext Tris Buffer
E7496AAVIAL
4
1 x 24 ml
Not Applicable
NEBNext Oligo d(T)25 Beads
E7499AAVIAL
4
1 x 1.92 ml
Not Applicable
特性和用法
需要但不提供的材料
96-well 0.2 ml PCR Plates and Microseal® 'B' Adhesive Sealer (BioRad MSB-1001) or 0.2 ml RNase-free tube.
Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)
Thermal cycler or heat block
Bioanalyzer® (Agilent Technologies, Inc.) or similar instrument and consumables.
优势和特性
应用特性
Isolation of poly(A)+ RNA transcript from Total RNA for RNA library preparation and sequencing.
操作说明、说明书 & 用法
操作说明
Where can I find protocols for using NEBNext® RNA Library Prep reagents?
说明书
产品说明书包含产品使用的详细信息、产品配方和质控分析。
manualE7490
使用指南
Getting Started with RNA-Sequencing (RNA-Seq)
FAQs & 问题解决指南
FAQs
Can I isolate poly(A)+ mRNA directly from tissue lysis using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)?
Do I need two rounds of binding?
Can I use the Oligo (dT)25 Magnetic Beads (NEB# S1419S) to isolate poly(A)+ mRNA for Next Generation Sequence applications?
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
产品信息
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
产品类别:
Discontinued (<3 years)
特性和用法
贮存溶液
0.1% BSA 0.05% Tween® 20 0.02% NaN3 1 ml suspension in PBS Buffer pH 7.4 @ 25°C
支持介质
1 µm nonporous superparamagnetic microparticle
结合容量
5 μg/mg Mouse lgG/mg Goat anti-Mouse Ig6 Beads.
可再生
Yes
货运单
4°C
相关产品
相关产品
12 孔磁性分离架
6 孔磁性分离架
Protein A Magnetic Beads
Protein G 磁珠
链霉亲和素磁珠
注意事项
The amount of antibody required for optimal coating of the beads will vary with the mAB affinity and the antigen density on the cell surface. Optimal experimental conditions should be determined by titration on an individual basis.
操作说明、说明书 & 用法
操作说明
Goat Anti-Mouse IgG Magnetic Beads Protocol
FAQs & 问题解决指南
FAQs
Do I need to block the beads or are they pre-blocked?
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
产品信息
An affinity matrix for the small-scale immunomagnetic separation and purification of rabbit IgG’s. Goat Anti-Rabbit IgG is covalently coupled to a nonporous superparamagnetic particle. This secondary antibody binds the heavy chain of all rabbit IgG subclasses and is suitable for immunoassays that employ a rabbit IgG primary polyclonal antibody. Cell separations and sorting can be accomplished using a rabbit IgG antibody to defined cell surface antigens.
Supplied as a 1 ml suspension in PBS Buffer (pH 7.4), containing 0.05% Tween 20, 0.1% BSA and 0.05% NaN3.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
产品类别:
Discontinued (<3 years)
特性和用法
贮存溶液
0.05% Tween® 20 0.02% NaN3 1 ml suspension in PBS Buffer pH 7.4 @ 25°C
支持介质
1 µm nonporous superparamagnetic microparticle
结合容量
5 μg Rabbit lgG/mg Goat Anti-Rabbit IgG Magnetic Beads
可再生
Yes
相关产品
相关产品
12 孔磁性分离架
6 孔磁性分离架
50 ml 磁性分离架
Amylose 磁珠
几丁质磁珠
Protein A Magnetic Beads
Protein G 磁珠
链霉亲和素磁珠
注意事项
The amount of antibody required for optimal coating of the beads will vary with the PAb affinity and the antigen density on the cell surface. Optimal experimental conditions should be determined by titration on an individual basis.
操作说明、说明书 & 用法
操作说明
Goat Anti-Rabbit IgG Magnetic Beads Protocol
FAQs & 问题解决指南
FAQs
Do I need to block the beads or are they pre-blocked?
