上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
产品信息
产品来源
Isolated from Lysobacter enzymogenes
重组
Endoproteinase LysC should be reconstituted in 200 μl double-distilled water to make a 100 ng/μl solution in 10 mM Tris-HCl, pH 8.0. Rapid autolysis is a function of enzyme concentration.
- 产品类别:
- Proteases Products,
- Proteome Analysis Products
- 应用:
- Glycomics and glycoproteomics,
- Protein Digestion,
- Glycobiology & Proteomics,
Proteomics
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产品组分信息
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
P8109S -20 Endoproteinase LysC P8109SVIAL -20 1 x 0.02 mg Not Applicable
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特性和用法
使用浓度
100 ng/μl
分子量
实际: 30000 daltons
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优势和特性
应用特性
- Digestion of proteins for proteomic analysis by Mass Spectrometry
- Protein and peptide identification
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相关产品
相关产品
- Trypsin-ultra™, Mass Spectrometry Grade
- Endoproteinase GluC
- Endoproteinase AspN
- α-Lytic Protease
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注意事项
- Storage Conditions: Supplied in dry format from a Tris-HCl buffer. The solution can be stored at 4°C for several days or in single-use aliquots at –20°C for several months. Use only freshly reconstituted protease for best results.
- LysC is active over a wide pH range, pH 5-12, depending on the application. Ammonium bicarbonate buffer may also be used in place of Tris-HCl.
操作说明、说明书 & 用法
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操作说明
- In-gel Digestion Protocol for Endoproteinase LysC (P8109)
- Typical Reaction Conditions for Endoproteinase LysC (P8109)
工具 & 资源
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选择指南
- Protease Selection Chart
- Substrate-based Ligase Selection Chart
FAQs & 问题解决指南
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FAQs
- What buffer conditions can Endoproteinase LysC be used in? Are there any special applications for which LysC is especially good?
- How much Endoproteinase LysC do I really need to digest my protein?
- Can Endoproteinase LysC used in a double digest with Trypsin-ultra?
- Can Endoproteinase LysC be used in acidic buffer conditions?