Endoproteinase LysC |NEB酶试剂 New England Biolabs

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产品信息

Endoproteinase LysC is a serine endoproteinase, isolated from Lysobacter enzymogenes, that cleaves peptide bonds at the carboxyl side of lysine. LysC is a sequencing grade enzyme and is suitable for proteomics and glycobiology applications.

Endoproteinase LysC |
A 16 hour native LysC digestion of Issatchenkia orientalis Cytochrome c using a 1:50 enzyme to substrate ratio, followed by LC-MS with a Thermo™ LTQ Orbitrap XL, identified 79 peptides with 85% sequence coverage.

产品来源

Isolated from Lysobacter enzymogenes

重组

Endoproteinase LysC should be reconstituted in 200 μl double-distilled water to make a 100 ng/μl solution in 10 mM Tris-HCl, pH 8.0. Rapid autolysis is a function of enzyme concentration.

产品类别:
Proteases Products,
Proteome Analysis Products

应用:
Glycomics and glycoproteomics,
Protein Digestion,
Glycobiology & Proteomics,

Proteomics

  • 产品组分信息

    本产品提供以下试剂或组分:

    NEB # 名称 组分货号 储存温度 数量 浓度
    • P8109S     -20    
        Endoproteinase LysC P8109SVIAL -20 1 x 0.02 mg Not Applicable

  • 特性和用法

    使用浓度

    100 ng/μl

    分子量

    实际: 30000 daltons

  • 优势和特性

    应用特性

    • Digestion of proteins for proteomic analysis by Mass Spectrometry
    • Protein and peptide identification

  • 相关产品

    相关产品

    • Trypsin-ultra™, Mass Spectrometry Grade
    • Endoproteinase GluC
    • Endoproteinase AspN
    • α-Lytic Protease

  • 注意事项

    1. Storage Conditions: Supplied in dry format from a Tris-HCl buffer. The solution can be stored at 4°C for several days or in single-use aliquots at –20°C for several months. Use only freshly reconstituted protease for best results.
    2. LysC is active over a wide pH range, pH 5-12, depending on the application. Ammonium bicarbonate buffer may also be used in place of Tris-HCl.

操作说明、说明书 & 用法

  • 操作说明

    1. In-gel Digestion Protocol for Endoproteinase LysC (P8109)
    2. Typical Reaction Conditions for Endoproteinase LysC (P8109)

工具 & 资源

  • 选择指南

    • Protease Selection Chart
    • Substrate-based Ligase Selection Chart

FAQs & 问题解决指南

  • FAQs

    1. What buffer conditions can Endoproteinase LysC be used in? Are there any special applications for which LysC is especially good?
    2. How much Endoproteinase LysC do I really need to digest my protein?
    3. Can Endoproteinase LysC used in a double digest with Trypsin-ultra?
    4. Can Endoproteinase LysC be used in acidic buffer conditions?