产品信息

 
IdeZ Protease (IgG-specific) is a recombinant antibody specific protease that recognizes all human, sheep, monkey, and rabbit IgG subclasses, specifically cleaving at a single recognition site below the hinge region, yielding a homogenous pool of F(ab´)2 and Fc fragments. IdeZ Protease more effectively cleaves murine IgG2a than IdeS.

产品来源

Cloned from Streptococcus equi subspecies zooepidemicus and expressed in E. coli. 

产品类别:

Proteases Products,

Proteome Analysis Products

应用:

Proteomics

• 产品组分信息

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  P0770S     -20       IdeZ Protease (IgG-specific) P0770SVIAL -20 1 x 0.05 ml 80,000 units/ml   GlycoBuffer 2 B3704SVIAL -20 1 x 1 ml 10 X

• 特性和用法

  单位定义

  One unit is defined as the amount of enzyme required to cleave > 95% of 1 µg of human IgG, in 15 minutes at 37°C in a total reaction volume of 10 µl.

  反应条件

  1X GlycoBuffer 2
  Incubate at 37°C

  1X GlycoBuffer 2
  50 mM Sodium Phosphate
  (pH 7.5 @ 25°C)

  贮存溶液

  20 mM Tris-HCl
  50 mM NaCl
  1 mM EDTA
  pH 7.5 @ 25°C

  热失活

  65°C for 10 minutes

  分子量

  理论上的: 35578 daltons

  单位活性检测条件

  Two fold dilutions of IdeZ Protease (IgG-specific) are incubated with 1 μg of human IgG and 1X GlycoBuffer 2 in a 10 µl reaction. The reaction mix is incubated for 15 minutes at 37°C. Separation of reaction products are visualized by SDS-PAGE.

  存储注意事项

  • Avoid repeated freeze/thaw cycles.

• 相关产品

  相关产品

  • Rapid 快速 PNGase F
  • Endo S
  • Trypsin-ultra™, Mass Spectrometry Grade
  • p6043-rapid-pngase-f-antibody-standard
  • p0711-rapid-pngase-f-non-reducing-format

• 注意事项

  1. Store at -20°C for up to two years.
  2. IdeZ Protease efficiently cleaves human, humanized, chimeric, sheep,rabbit and monkey IgG as well as mouse IgG2a and IgG3. IdeZ Proteasewill also cleave Fc-fusion proteins, such as Enbrel.
  3. IdeZ Protease does not cleave mouse IgG1 or IgG2b, rat, porcine, bovineor goat IgG. It also does not cleave non-IgG isotypes including IgA,IgM, IgD and IgE.

操作说明、说明书 & 用法

• 操作说明

  1. Reaction Conditions for IdeZ Protease (IgG-specific) (P0770)
  2. Reaction conditions for Simultaneous Digestion of IgG with IdeZ Protease (IgG-specific) and PNGase F (fragmentation and deglycosylation) (P0770)

FAQs & 问题解决指南

• FAQs

  1. What is the advantage of IdeZ over IdeS?
  2. Is IdeZ Protease active in Phosphate Buffered Saline (PBS)?
  3. Can protein A magnetic beads (NEB# S1425S) be used to create Fc and Fab fragment pools after cleavage with IdeZ Protease?
  4. Can IdeZ Protease cleave IgGs other than human?
  5. Can IdeZ Protease cleave Ig isotypes other than IgG?
  6. Can PNGase F be used together with IdeZ Protease under native conditions to deglycosylate the Fc portion of an antibody?
  7. What is the cleavage site for IdeZ Protease (IgG-specific)?