HF13502S54-美国Millipore密理博 硝酸纤维素膜(NC膜系列)Hi-Flow Plus 135

产品型号HF13502S54

品       牌密理博

产品简介

美国Millipore密理博 硝酸纤维素膜(NC膜系列)Hi-Flow Plus 135,货号:HF13502S54;宽5.4cm,长:100m,Millipore 135硝酸纤维素膜

详情介绍

详情:

描述
产品编号 HF13502S54
品牌 Hi-Flow Plus
描述 Hi-Flow Plus 135 2mil Backing Master Roll slit width 5.4cm
概述 Full Master roll (30.5cm wide) of Hi-Flow Plus Cellulose Ester membranes with nominal capillary flow time of 135 seconds/4 cm slit to stated width
应用 Lateral Flow, Assay Development, lateral flow immunoassays, lateral flow assay device, Immunoassay IVDs
宽度 5.4cm
长度 100 m (328 ft.)

 

HANNA哈纳 HI6016pH校准液1.679

HANNA哈纳 HI6016pH校准液1.679

HANNA哈纳 HI6016pH校准液1.679

  • 商品品牌: HANNA哈纳
    商品编号:HI6016
  • 商品价格: 请与我们联系
  • HANNA哈纳 HI6016pH校准液1.679-HANNA哈纳-HI6016

    实验室耗材|||标准品|||PH标准溶液|||HANNA哈纳HI6016pH校准液1.679
    PH校准缓冲液性 校准液

    HANNA哈纳 HI6016pH校准液1.679


    商品属性

    商品属性
    商品名称 HANNA哈纳 HI6016pH校准液1.679-HI6016-HANNA哈纳
    型号 HI6016
    类别 实验室耗材|||标准品|||PH标准溶液|||HANNA哈纳HI6016pH校准液1.679
    品牌 HANNA哈纳
    品牌简介 HANNA哈纳
    关键字 PH校准缓冲液性 校准液,缓冲液

    HANNA哈纳 HI6016pH校准液1.679

    Hi-T7 耐热 RNA 聚合酶(高浓度) |NEB酶试剂 New England Biolabs

    上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。

    产品信息

    Hi-T7 耐热 RNA 聚合酶(高浓度)的浓度为标准 Hi-T7 耐热 RNA 聚合酶(NEB#M0658)的 20 倍,是希望自己建立和优化体外转录反应体系的有经验用户的理想选择。该酶配有 Hi-T7 耐热 RNA 聚合酶反应缓冲液和氯化镁溶液,可进一步调试不同条件,例如核苷酸浓度。

    体外转录除了 RNA 聚合酶外还涉及多种成分,包括核糖核苷酸、无机焦磷酸酶和 RNase 抑制剂,这些均可单独购买。详细信息请参考“相关产品”部分

    Hi-T7 耐热 RNA 聚合酶是一种经基因工程改造的 DNA 依赖性 RNA 聚合酶,对 T7 噬菌体启动子具有高度特异性,跟野生型噬菌体 T7 RNA 聚合酶相比,它可在更高的温度下进行反应。Hi-T7 耐热 RNA 聚合酶的最佳温度为 50-52°C。

    产品来源

    An E.coli strain that carries an engineered His-tag T7 RNA Polymerase gene.

    产品类别:
    RNA Synthesis In vitro Transcription (IVT)

    应用:
    Transcription-Mediated and NASBA Amplification

    • 产品组分信息

      本产品提供以下试剂或组分:

      NEB # 名称 组分货号 储存温度 数量 浓度
      • M0470T     -20    
          Hi-T7® RNA Polymerase (High Concentration) M0470TVIAL -20 1 x 0.05 ml 1,000,000 units/ml
          Hi-T7® RNA Polymerase Reaction Buffer B0658AVIAL -20 1 x 1 ml Not Applicable
          MgCl2 Solution B2534AVIAL -20 1 x 0.5 ml 0.2 M

    • 特性和用法

      单位定义

      One unit is defined as the amount of enzyme required to incorporate 1 nmol of ATP into acid-insoluble material in 1 hour at 50°C

      反应条件

      1X Hi-T7® RNA Polymerase Reaction Buffer
      Incubate at 50°C

      1X Hi-T7® RNA Polymerase Reaction Buffer
      40 mM Tris-HCl
      4 mM MgCl2
      50 mM NaCl
      2 mM spermidine
      1 mM DTT
      (pH 7.5 @ 25°C)

      贮存溶液

      50 mM Tris-HCl
      100 mM NaCl
      1 mM EDTA
      1 mM DTT
      50% Glycerol
      0.1% (w/v) Triton® X-100
      pH 7.9 @ 25°C

      单位活性检测条件

      1X Hi-T7 RNA Polymerase Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP and 1 µg T7 phage DNA in 50 µl reaction volume.

    • 优势和特性

      应用特性

      • mRNA 用于体外翻译、显微注射以及转染
      • 放射标记的 RNA 探
      • 非同位素 RNA 标记
      • 靶基因的向导 RNA
      • RNA 的结构、加工以及催化作用的研究
      • 基因表达实验中的反义 RNA
      • RNA 扩增
      • 等温扩增

    • 相关产品

      相关产品

      • rNTP 套装
      • rNTP 混合液
      • 小鼠 RNase 抑制剂
      • rNTP 混合液
      • T2040 Monarch RNA Cleanup Kit 50 ug
      • m0251-t7-rna-polymerase
      • 无机焦磷酸酶(大肠杆菌)

    操作说明、说明书 & 用法

    • 操作说明

      1. Protocol for Standard RNA Synthesis

    • 应用实例

      • Scaling of High-Yield In vitro Transcription Reactions for Linear Increase of RNA Production

    FAQs & 问题解决指南

    • FAQs

      1. Can I use a different reaction buffer for in vitro transcription with Hi-T7 RNA Polymerase?
      2. Does Hi-T7 RNA Polymerase recognize the same promoter sequence as the wild-type T7 RNA Polymerase from bacteriophage?
      3. At what temperature is Hi-T7 RNA Polymerase active?
      4. What are the main causes of reaction failure when using Hi-T7 RNA Polymerase?
      5. What templates can be used for in vitro transcription with Hi-T7 RNA Polymerase?
      6. Can Hi-T7 RNA Polymerase transcribe off of an uncut plasmid?

    Hi-T7® RNA Polymerase |NEB酶试剂 New England Biolabs

    上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。

    产品信息

    Hi-T7 RNA Polymerase is an engineered DNA-dependent RNA polymerase that is highly specific for T7 phage promoters and is designed to function at higher temperatures than the wild-type bacteriophage T7 RNA Polymerase. Hi-T7 RNA Polymerase functions at an optimal temperature of 50-52°C.

    产品来源

    An E. coli strain that carries an engineered His-tag T7 RNA Polyermase gene.

    产品类别:
    RNA Synthesis In vitro Transcription (IVT)

    • 产品组分信息

      本产品提供以下试剂或组分:

      NEB # 名称 组分货号 储存温度 数量 浓度
      • M0658S     -20    
          Hi-T7® RNA Polymerase M0658SVIAL -20 1 x 0.1 ml 50,000 units/ml
          Hi-T7® RNA Polymerase Reaction Buffer B0658AVIAL -20 1 x 1 ml Not Applicable

    • 特性和用法

      单位定义

      One unit is defined as the amount of enzyme required to incorporate 1 nmol of ATP into acid-insoluble material in 1 hour at 50°C.

      Reaction Conditions
      1X Hi-T7 RNA Polymerase Reaction Buffer, supplemented with 0.5 mM each of ATP, UTP, GTP, CTP, and DNA template containing the T7 RNA Polymerase promoter.  Incubate at 37-56°C for 1 hour.  Optimal incubation temperature is 50-52°C.

      反应条件

      1X Hi-T7® RNA Polymerase Reaction Buffer
      Supplement with 0.5 mM ATP, 0.5 mM UTP , 0.5 mM GTP 和 0.5 mM CTP 
      Incubate at 50°C

      1X Hi-T7® RNA Polymerase Reaction Buffer
      40 mM Tris-HCl
      4 mM MgCl2
      50 mM NaCl
      2 mM spermidine
      1 mM DTT
      (pH 7.5 @ 25°C)

      贮存溶液

      50 mM Tris-HCl
      100 mM NaCl
      1 mM EDTA
      1 mM DTT
      0.1% (w/v) Triton® X-100
      50% Glycerol
      pH 7.9 @ 25°C

      单位活性检测条件

      1X Hi-T7 RNA Polymerase Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP and 1 µg T7 phage DNA in 50 µl reaction volume.

    • 优势和特性

      应用特性

      • Radiolabeled RNA probes
      • Non-isotopic RNA labeling
      • Guide RNA for gene targeting
      • mRNA for in vitro translation, microinjection and transfection 
      • RNA structure, processing and catalysis studies
      • RNA amplification
      • Isothermal amplification
      • Anti-sense RNA for gene expression

    • 相关产品

      相关产品

      • m0251-t7-rna-polymerase
      • 小鼠 RNase 抑制剂
      • rNTP 套装
      • rNTP 混合液

    操作说明、说明书 & 用法

    • 操作说明

      1. Protocol for Standard RNA Synthesis with Hi-T7 RNA Polymerase (NEB #M0658)

    • 应用实例

      • Scaling of High-Yield In vitro Transcription Reactions for Linear Increase of RNA Production

    FAQs & 问题解决指南

    • FAQs

      1. Can I use a different reaction buffer for in vitro transcription with Hi-T7 RNA Polymerase?
      2. Does Hi-T7 RNA Polymerase recognize the same promoter sequence as the wild-type T7 RNA Polymerase from bacteriophage?
      3. At what temperature is Hi-T7 RNA Polymerase active?
      4. What are the main causes of reaction failure when using Hi-T7 RNA Polymerase?
      5. What templates can be used for in vitro transcription with Hi-T7 RNA Polymerase?
      6. Can Hi-T7 RNA Polymerase transcribe off of an uncut plasmid?