标签归档：gdna

Monarch® gDNA Elution Buffer II |NEB酶试剂 New England Biolabs

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上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

The Monarch gDNA Elution Buffer II is supplied as a component of the Monarch HMW DNA Extraction Kits (NEB #T3050 and #T3060). It is formulated for long term storage of genomic DNA and is made of 10mM Tris, pH 9.0, 0.5 mM EDTA.

产品类别:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T3056L     25    
        Monarch® gDNA Elution Buffer II T3056-1 25 2 x 12 ml Not Applicable

  • 相关产品

    相关产品

    • Monarch 高分子量 DNA 提取试剂盒（细胞和血液）
    • T3060 Monarch HMW DNA Extraction Kit for Tissue
    • Monarch RBC 裂解液
    • Monarch DNA 捕获珠
    • T3054 Monarch gDNA Nuclei Prep and Lysis Buffer Pack
    • T3055 Monarch Precipitation Enhancer
    • T3061 Monarch HMW gDNA Tissue Lysis Buffer
    • T3062 Monarch Protein Separation Solution
    • T3000 Monarch Pestle Set
    • Monarch 捕获珠分离管
    • Monarch® 收集管 II
    • Monarch 2 ml 离心管
    • T3015 Monarch gDNA Wash Buffer

操作说明、说明书 & 用法

  • 操作说明

    1. Download Quick Protocol Card for Cells
    2. Download Quick Protocol Card for Blood
    3. Download Quick Protocol Card for Tissue
    4. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Cells (NEB #T3050)
    5. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Blood (NEB #T3050)
    6. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Bacteria (NEB #T3060)
    7. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Tissue (NEB #T3060)
    8. Protocol for UHMW DNA Cleanup in the Oxford Nanopore Technologies® UL Library Prep Workflow

  • 使用指南

    • Homogenization of High Molecular Weight DNA (HMW DNA) Samples After Elution
    • Measuring, Analyzing & Storing High Molecular Weight DNA (HMW DNA) Samples

FAQs & 问题解决指南

  • FAQs

    1. Which measurement system is best used for assessing the concentration of HMW DNA?
    2. I am having trouble dissolving my HMW DNA after isolation. Do you have any tips on increasing the solubility?

  • 问题解决指南

    • Troubleshooting Guide for Monarch HMW DNA Extraction Kits (NEB #T3050 and #T3060)

Monarch® gDNA Nuclei Prep & Lysis Buffer Pack |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

The Monarch gDNA Nuclei Prep & Lysis Buffer Pack contains two lysis buffers used in the Monarch HMW DNA Extraction Kit for Cells & Blood (NEB #T3050). The Nuclei Prep Buffer is a mild lysis buffer used first to lyse the cell membrane and release the contents of the cytoplasm while the nuclei are kept intact. It is used pre-mixed with the RNase A enzyme included in the kit. After the RNA from the cytoplasm is digested by RNase A, the Nuclei Lysis Buffer, pre-mixed with the included Proteinase K, is used to lyse the nuclei and release the HMW genomic DNA. This two-step lysis ensures that RNA in the cytoplasm is effectively degraded before the viscous genomic DNA from the nuclei is released. 20 ml of each buffer is included in this buffer pack. The Nuclei Prep Buffer is used chilled to 4°C, and it can be stored at 4°C for convenience if desired.

产品类别:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T3054L     25    
        Monarch® gDNA Nuclei Lysis Buffer T3053-1 25 2 x 10 ml Not Applicable
        Monarch® gDNA Nuclei Prep Buffer T3052-1 25 2 x 10 ml Not Applicable

  • 相关产品

    相关产品

    • Monarch 高分子量 DNA 提取试剂盒（细胞和血液）
    • T3060 Monarch HMW DNA Extraction Kit for Tissue
    • Monarch RBC 裂解液
    • T3056 Monarch gDNA Elution Buffer II
    • Monarch DNA 捕获珠
    • T3056 Monarch gDNA Elution Buffer II
    • Monarch 捕获珠分离管
    • Monarch® 收集管 II
    • p8107-proteinase-k-molecular-biology-grade
    • Monarch 2 ml 离心管
    • T3015 Monarch gDNA Wash Buffer

操作说明、说明书 & 用法

  • 操作说明

    1. Download Quick Protocol Card for Cells
    2. Download Quick Protocol Card for Blood
    3. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Cells (NEB #T3050)
    4. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Blood (NEB #T3050)
    5. Protocol for UHMW DNA Cleanup in the Oxford Nanopore Technologies® UL Library Prep Workflow

FAQs & 问题解决指南

  • 问题解决指南

    • Troubleshooting Guide for Monarch HMW DNA Extraction Kits (NEB #T3050 and #T3060)

Monarch® gDNA 提取离心柱 |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

Monarch gDNA 提取离心柱是 Monarch 基因组 DNA 提取试剂盒（NEB #T3010）的一个组分，可从各种生物样本中纯化多达 30 µg 的 DNA。离心柱的设计没有使用固定环，确保不会出现缓冲液残留及交叉污染。DNA 可在低至 35 μl 的体积中洗脱，洗脱后可随时用于各种下游应用，包括终点 PCR、qPCR 和 NGS DNA 文库制备。与传统离心柱相比，Monarch 离心柱使用的塑料更少，这能减少离心柱的环境足迹，却不会影响性能。提供 100 根离心柱以及 200 根 Monarch 收集管 II（NEB #T2018）。

产品类别:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products

应用:
Nucleic Acid Purification

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T3017L     25    
        Monarch® gDNA Purification Columns T3017-1 25 2 x 50 columns Not Applicable
        Monarch® Collection Tubes II T2018-1 25 4 x 50 tubes Not Applicable

操作说明、说明书 & 用法

  • 操作说明

    1. Quick Protocol for Extraction and Purification of Genomic DNA
    2. Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
    3. Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
    4. Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
    5. Protocol for Genomic DNA Cleanup (NEB #T3010)
    6. Genomic DNA Purification from Gram-positive Bacteria and Archaea (NEB #T3010)
    7. Genomic DNA Purification from Yeast (NEB #T3010)
    8. Genomic DNA Purification from Saliva (NEB #T3010)
    9. Genomic DNA Purification from Buccal Swabs (NEB #T3010)
    10. Genomic DNA Purification from Gram-negative Bacteria (NEB #T3010)
    11. Download Quick Protocol Card T3010
    12. Genomic DNA Purification from Insects

  • 使用指南

    • Choosing Input Amounts for the Monarch Genomic DNA Purification Kit
    • Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch Genomic DNA Purification Kit
    • Guidelines for Handling Tissue Samples when using the Monarch Genomic DNA Purification Kit

  • 应用实例

    • A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers

FAQs & 问题解决指南

  • FAQs

    1. What is the maximum binding capacity of the Monarch® Genomic DNA Purification Kit columns?
    2. What is the minimum elution volume that can be used with the Monarch® Genomic DNA Purification Kit columns?
    3. How important is it to pre-heat the elution buffer to 60°C? Would 56°C be OK?
    4. Can I get better recovery with the Monarch® Genomic DNA Purification Kit if I do a second elution with my eluent from the first elution?
    5. Can I use a different elution buffer than the one supplied with the Monarch Genomic DNA Purification Kit (NEB #T3010)?
    6. What size gDNA can be purified with the Monarch® Genomic DNA Purification Kit?
       
    7. What is the minimal input amount that can be used with the Monarch® Genomic DNA Purification Kit?
    8. Can I use the Monarch® Genomic DNA Purification Kit to clean up gDNA that was extracted with phenol/chloroform?
    9. Can I use the Monarch® Genomic DNA Purification Kit for gDNA cleanup?
       
    10. How can I assess gDNA integrity and purity?
    11. Can I omit the low-speed binding centrifugation step to save time?
    12. Can I omit the RNase digestion step from the protocol? 
    13. Is Monarch-purified gDNA suitable for long-range PCR?
    14. When working with other commercial silica column-based kits, I occasionally see a white precipitate in the eluate. What is this?
    15. Is Monarch-purified gDNA compatible with Next Generation Sequencing (NGS) platforms like PacBio and nanopore?
    16. Why did the silica membrane on my Monarch Genomic DNA Column (NEB #T3017) dislodge during my genomic DNA prep?
    17. Can the Monarch Genomic DNA Purification Kit (NEB #T3010) be used to isolate gDNA from drosophila and other insects?
    18. Can the Monarch Genomic DNA Purification Kit (NEB #T3010) be used to isolate gDNA from plants?
    19. Can the Monarch Genomic DNA Purification Kit (NEB #T3010) be used to isolate gDNA from plasma, serum and urine?
    20. Can the Monarch Genomic DNA Purification Kit (NEB #T3010) be used for isolating cfDNA?
    21. Can the Monarch Genomic DNA Purification Kit (NEB #T3010) be used with a vacuum manifold?

  • 问题解决指南

    • Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)

Monarch® gDNA 洗脱缓冲液 |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

Monarch gDNA 洗脱缓冲液含 10 mM Tris-HCl（pH 9.0）、0.1 mM EDTA，适用于长期贮存基因组 DNA。添加 EDTA 和溶液 pH 可以使核酸酶失活。

产品类别:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products

应用:
Nucleic Acid Purification

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T3016L     25    
        Monarch® gDNA Elution Buffer T3016-2 25 1 x 34 ml Not Applicable

操作说明、说明书 & 用法

  • 操作说明

    1. Quick Protocol for Extraction and Purification of Genomic DNA
    2. Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
    3. Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
    4. Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
    5. Protocol for Genomic DNA Cleanup (NEB #T3010)
    6. Genomic DNA Purification from Gram-positive Bacteria and Archaea (NEB #T3010)
    7. Genomic DNA Purification from Yeast (NEB #T3010)
    8. Genomic DNA Purification from Saliva (NEB #T3010)
    9. Genomic DNA Purification from Buccal Swabs (NEB #T3010)
    10. Genomic DNA Purification from Gram-negative Bacteria (NEB #T3010)

  • 使用指南

    • Choosing Input Amounts for the Monarch Genomic DNA Purification Kit
    • Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch Genomic DNA Purification Kit
    • Guidelines for Handling Tissue Samples when using the Monarch Genomic DNA Purification Kit

FAQs & 问题解决指南

  • FAQs

    1. What is the composition of each buffer provided with the Monarch® Genomic DNA Purification Kit?
    2. What is the minimum elution volume that can be used with the Monarch® Genomic DNA Purification Kit columns?
    3. How important is it to pre-heat the elution buffer to 60°C? Would 56°C be OK?
    4. Can I get better recovery with the Monarch® Genomic DNA Purification Kit if I do a second elution with my eluent from the first elution?
    5. Can I use a different elution buffer than the one supplied with the Monarch Genomic DNA Purification Kit (NEB #T3010)?
    6. What size gDNA can be purified with the Monarch® Genomic DNA Purification Kit?
       
    7. How can I assess gDNA integrity and purity?
    8. Can I omit the low-speed binding centrifugation step to save time?
    9. How should I store purified gDNA samples?
    10. When working with other commercial silica column-based kits, I occasionally see a white precipitate in the eluate. What is this?
    11. Is Monarch-purified gDNA compatible with Next Generation Sequencing (NGS) platforms like PacBio and nanopore?

  • 问题解决指南

    • Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)