产品信息

• 产品组分信息

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  T2017L     25       Monarch® gDNA Removal Columns T2017-1 25 2 x 50 columns Not Applicable   Monarch® Spin Collection Tubes T2118-1 25 2 x 50 tubes Not Applicable

• 相关产品

  相关产品

  • Luna^® 通用一步法 RT-qPCR 试剂盒
  • Luna^® 通用探针一步法 RT-qPCR 试剂盒
  • e6560-protoscript-ii-first-strand-cdna-synthesis-kit
  • NEBNext^® Ultra^™ II RNA 非链特异性文库制备试剂盒
  • e7760-nebnext-ultra-ii-directional-rna-library-prep-kit-for-illumina
  • e7765-nebnext-ultra-ii-directional-rna-library-prep-with-sample-purification-beads
  • NEBNext^® rRNA 去除试剂盒 – 人/小鼠/大鼠（含 RNA 纯化磁珠）
  • e7490-nebnext-polya-mrna-magnetic-isolation-module
  • m0368-protoscript-ii-reverse-transcriptase
  • t1010-monarch-plasmid-miniprep-kit
  • Monarch® DNA 胶回收试剂盒
  • Monarch® PCR & DNA 纯化试剂盒（5 μg）

  单独销售的组分

  • Monarch® RNA 提取离心柱
  • Monarch^® DNA/RNA 保护试剂
  • Monarch^® 总 RNA 小量提取试剂盒
  • Monarch^® 收集管 II
  • Monarch^® RNA 裂解液
  • Monarch^® RNA 初始洗涤缓冲液
  • t2014-monarch-rna-wash-buffer
  • Monarch^® 总 RNA 小量提取酶套装
  • Nuclease-free Water

操作说明、说明书 & 用法

• 操作说明

  1. Quick Protocol for Total RNA Extraction (NEB #T2010)
  2. Download Quick Protocol Card T2010
  3. FFPE Tissue Deparaffinization and Subsequent RNA Purification Using the Monarch Total RNA Miniprep Kit  (NEB #T2010)
  4. Total RNA Purification from Cultured Mammalian Cells using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  5. Total RNA Purification from Tissues and Leukocytes using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  6. Total RNA Purification from Tough-to-Lyse Samples (bacteria, yeast, plant, etc.) using the Monarch Total RNA Miniprep Kit (NEB #T2010)

• 使用指南

  • General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
  • Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
  • Guidelines for Purification of RNA from Cultured Mammalian Cells
  • Guidelines for RNA Purification from Bacteria
  • Guidelines for RNA Purification from Leukocytes (WBCs, PBMCs)
  • Guidelines for RNA Purification from Plants
  • Guidelines for RNA Purification from Tissues 
  • Guidelines for RNA Purification from Whole Blood
  • Guidelines for RNA Purification from Yeast
  • Guidelines for Sample Stabilization Using the Monarch DNA/RNA Protection Reagent

FAQs & 问题解决指南

• FAQs

  1. How can I tell the difference between the gDNA Removal Column and the RNA Purification Column?
  2. Can I skip the gDNA removal column step of the Monarch Total RNA Miniprep Kit protocol?
  3. Can the genomic DNA captured on the Monarch gDNA Removal Column (NEB #T2017) be eluted and used in downstream applications?
  4. Is the Monarch Total RNA Miniprep Kit compatible with Luna RT-qPCR Reagents?
  5. Is the Monarch Total RNA Miniprep Kit compatible with NEBNext reagents for RNA Library Prep?
  6. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from bacteria?
  7. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from plants?
  8. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from yeast?
  9. What is the lowest volume in which I can elute my RNA with the Monarch Total RNA Miniprep Kit (NEB #T2010)?

• 问题解决指南

  • Troubleshooting Guide for Total RNA Extraction & Purification