产品信息

• 产品组分信息

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  E8022S     4       Amylose Resin High Flow E8022SVIAL 4 1 x 15 ml Not Applicable
  E8022L     4       Amylose Resin High Flow E8022LVIAL 4 1 x 100 ml Not Applicable

• 特性和用法

  贮存溶液

  20% Ethanol

  结合容量

  >4 mg MBP5* -paramyosin δSal fusion protein / ml amylose high flow resin.

  可再生

  Yes

• 注意事项

  1. Store at 4°C. After use, resin should be stored in column buffer plus 0.02% sodium azide or 20% ethanol.
  2. Amylose Resin column should be washed with 5 volumes of column buffer before each use. 
     For optimum performance, load crude extract at < 60 cm/hour.
  3. When regenerating the column at 4°C, please note that 0.1% SDS can precipitate at that temperature. It is therefore recommended that the SDS solution be stored at room temperature until needed. The resin may be generated up to five times. 
  4. For a complete affinity purification protocol, download the pMAL Protein Fusion and Purification System technical bulletin (NEB #E8200) from www.neb.com.
  5. Regeneration: The packed resin may be regenerated by the following wash sequence: Water 3 – column volumes, 0.1% SDS – 3 column volumes, Water – 1 column volume, Column Buffer – 5 column volumes.

工具 & 资源

• 选择指南

  • Purification Beads, Columns and Resins

FAQs & 问题解决指南

• FAQs

  1. What gravity column do you recommend?
  2. Much of my fusion protein flows through the amylose column. Is there anything I can do to improve my fusion’s affinity for the amylose column?
  3. How many times can I use the amylose column?
  4. What is known about binding in the presence of nonionic detergents?
  5. Can I substitute a different buffer and/or salt concentration in the Column Buffer?
  6. I see my intact fusion protein by SDS-PAGE when I run cells boiled in Sample Buffer, but when I check the crude extract the fusion is degraded.
  7. When I run my purified fusion protein on SDS-PAGE, why do I see multiple bands instead of a single band of the expected MW?
  8. Can I perform a batch purification using the amylose resin?
  9. Can MBP fusions be purified in the presence of denaturants like urea or guanidine-HCl?
  10. Is the amylose resin damaged by storage at -20°C?
  11. What is the recommended flow rate and maximum pressure for Amylose Resin High Flow once packed into a column?