标签归档：exonuclease

Exonuclease VIII, truncated |NEB酶试剂 New England Biolabs

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上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

产品来源

An E. coli strain that carries a plasmid with genetic engineering active domain of Exonuclease VIII.

产品类别:
Exonucleases and Non-specific Endonucleases Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0545S     -20    
        Exonuclease VIII, truncated M0545SVIAL -20 1 x 0.1 ml 10,000 units/ml
        NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide from double-stranded DNA in a total reaction volume of 50 µl in 30 minutes at 37°C in 1X NEBuffer 4 with 0.15 mM sonicated duplex [3H] DNA.

    反应条件

    1X NEBuffer™ 4
    Incubate at 37°C

    1X NEBuffer™ 4
    50 mM Potassium Acetate
    20 mM Tris-acetate
    10 mM Magnesium Acetate
    1 mM DTT
    (pH 7.9 @ 25°C)

    贮存溶液

    50 mM Tris-HCl
    100 mM NaCl
    0.1 mM EDTA
    1 mM DTT
    0.1% Triton® X-100
    50% Glycerol
    pH 7.5 @ 25°C

    热失活

    70°C for 15 minutes

    单位活性检测条件

    1X NEBuffer 4 with 0.15 mM with sonicated duplex 3H- DNA.

  • 优势和特性

    应用特性

    •    Degradation of linear dsDNA while maintaining double stranded circular DNA.

  • 参考文献

    1. Chang, et al. (2001). J. Biol. Chem.. 46004–46010.

操作说明、说明书 & 用法

  • 操作说明

    1. Protocol for Exonuclease VIII, truncated (M0545)

工具 & 资源

  • 选择指南

    • Activities of Exonucleases and Non-specific Endonucleases
    • Common Applications for Exonucleases and Endonucleases
    • Properties of Exonucleases and Non-specific Endonucleases

  • Web 工具

    • Exo Selector

  • 研究摘要

    • Choosing the Right Exonuclease (2019)

FAQs & 问题解决指南

  • FAQs

    1. How does Exonuclease VIII, truncated differ from Lambda Exonuclease (NEB# M0262)?

    2. Will Exonuclease VIII, truncated cleave supercoiled dsDNA or covalently close circular form dsDNA?

    3. How does Exonuclease VIII, truncated differ from Exonuclease III (NEB# M0206)?
    4. Can DNA be blunted using Exonuclease VIII, truncated?
    5. What is the activity of Exonuclease VIII, truncated in these common buffers?

Lambda Exonuclease |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

重点

Isolated from a recombinant source
Highly processive 5’→3′ exonuclease
Supplied in 10X Reaction Buffer

产品来源

A genetic fusion of the E. coli Lambda Exonuclease gene with the gene encoding maltose binding protein (MBP). Following affinity chromatography, Lambda Exonuclease is cleaved from the fusion construct and purified away from MBP. 

产品类别:
Exonucleases and Non-specific Endonucleases Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0262S     -20    
        Lambda Exonuclease M0262SVIAL -20 1 x 0.2 ml 5,000 units/ml
        Lambda Exonuclease Reaction Buffer B0262SVIAL -20 1 x 1.5 ml 10 X
    • M0262L     -20    
        Lambda Exonuclease M0262LVIAL -20 1 x 1 ml 5,000 units/ml
        Lambda Exonuclease Reaction Buffer B0262SVIAL -20 1 x 1.5 ml 10 X

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Lambda Exonuclease Reaction Buffer with 1 μg sonicated duplex [3H]-DNA.

    反应条件

    1X Lambda Exonuclease Reaction Buffer
    Incubate at 37°C

    1X Lambda Exonuclease Reaction Buffer
    67 mM Glycine-KOH
    2.5 mM MgCl2
    50 µg/ml BSA
    (pH 9.4 @ 25°C)

    使用浓度

    5,000 units/ml

    贮存溶液

    25 mM Tris-HCl
    50 mM NaCl
    1 mM DTT
    0.1 mM EDTA
    50% Glycerol
    pH 8 @ 25°C

    热失活

    75°C for 10 minutes

    分子量

    理论上的: 28000 daltons

    比活度

    50,000 units/mg

  • 相关产品

    相关产品

    • t1010-monarch-plasmid-miniprep-kit
    • Monarch® DNA 胶回收试剂盒
    • Monarch® PCR & DNA 纯化试剂盒（5 μg）

  • 注意事项

    1. Lambda 核酸外切酶在各种缓冲液中的活性如下所示：

      • NEBuffer 1：15%
      • NEBuffer 2：40%
      • NEBuffer 3：10%
      • NEBuffer 4：40%
      • CutSmart：50%
      • 核酸外切酶 I 反应缓冲液：100%
      • Thermopol 反应缓冲液：100%
    2. 5´ 羟基末端的酶切速度比 5´ 磷酸末端慢 20 倍。单链 DNA 的酶切速度比双链 DNA 慢 100 倍。

  • 参考文献

    1. Little, J.W. (1981). Gene Amplif. Anal. 2, 135-145.

操作说明、说明书 & 用法

  • 操作说明

    1. Protocol for Lambda Exonuclease (M0262)

  • 使用指南

    • Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

工具 & 资源

  • 选择指南

    • Activities of Exonucleases and Non-specific Endonucleases
    • Common Applications for Exonucleases and Endonucleases
    • Properties of Exonucleases and Non-specific Endonucleases

  • Web 工具

    • Exo Selector

  • 研究摘要

    • Choosing the Right Exonuclease (2019)

FAQs & 问题解决指南

  • FAQs

    1. What is the activity of M0262 Lambda Exonuclease in various NEB buffers?
    2. How Does T5 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
    3. How Does T7 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
    4. Will Lambda Exonuclease work in rCutSmart Buffer?
    5. Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?

T7 Exonuclease |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

重点

  • Isolated from a recombinant source
  • 5′ -> 3′ exonuclease
  • Supplied with 10X Reaction Buffer

产品来源

Purified from an E. coli strain containing a TYB12 intein fusion.

产品类别:
Exonucleases and Non-specific Endonucleases Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0263S     -20    
        T7 Exonuclease M0263SVIAL -20 1 x 0.1 ml 10,000 units/ml
        NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X
    • M0263L     -20    
        T7 Exonuclease M0263LVIAL -20 1 x 0.5 ml 10,000 units/ml
        NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X NEBuffer 4 with 0.15 mM sonicated duplex [3H]-DNA.

    反应条件

    1X NEBuffer™ 4
    Incubate at 25°C

    1X NEBuffer™ 4
    50 mM Potassium Acetate
    20 mM Tris-acetate
    10 mM Magnesium Acetate
    1 mM DTT
    (pH 7.9 @ 25°C)

    在不同缓冲液中的活性

    NEBuffer™ 1: 30%
    NEBuffer™ 2: 25%
    NEBuffer™ 3: 1%
    NEBuffer™ 4: 100%

    贮存溶液

    10 mM Tris-HCl
    5 mM DTT
    0.1 mM EDTA
    50% Glycerol
    pH 8 @ 25°C

    热失活

    否

  • 相关产品

    相关产品

    • t1010-monarch-plasmid-miniprep-kit
    • Monarch® DNA 胶回收试剂盒
    • Monarch® PCR & DNA 纯化试剂盒（5 μg）

    单独销售的组分

    • NEBuffer 4

  • 参考文献

    1. Kerr, C. and Sadowski, P.D. (1972). J. Biol. Chem. 247, 305-318.
    2. Shinozaki, K. and Okazaki, T. (1978). Nucl. Acids. Res. 5, 4245-4261.

操作说明、说明书 & 用法

  • 操作说明

    1. Protocol for T7 Exonuclease (M0263)

  • 使用指南

    • Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

工具 & 资源

  • 选择指南

    • Activities of Exonucleases and Non-specific Endonucleases
    • Common Applications for Exonucleases and Endonucleases
    • Properties of Exonucleases and Non-specific Endonucleases

  • Web 工具

    • Exo Selector

  • 研究摘要

    • Choosing the Right Exonuclease (2019)

FAQs & 问题解决指南

  • FAQs

    1. How Does T7 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
    2. How Does T7 Exonuclease differ from Exonuclease III (NEB# M0206)?
    3. What is the activity of T7 Exonuclease in the NEBuffers?
    4. Can T7 Exonuclease be heat inactivated?
    5. What is the best way to generate partials using T7 Exonuclease?
    6. Can DNA be blunted using T7 Exonuclease?
    7. Will T7 Exonuclease work in rCutSmart buffer?
    8. Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?

  • 实验技巧

    Addition of 6 phosphorothioates to the 5’ end of DNA can block activity up to 90%.

T5 Exonuclease | NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

T5 Exonuclease

This product is discontinued and has been replaced by T5 Exonuclease (NEB #M0663).

  • Catalog # M0363 was discontinued on October 02, 2020

T5 Exonuclease | NEB酶试剂 New England Biolabs

  • Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease
  • Initiates at the 5′ termini of linear or nicked double-stranded DNA
  • Cleaves linear or nicked double-stranded DNA in the 5′ to 3′ direction

T5 Exonuclease is ideal for:

  • Removal of incomplete ligation products from ligated circular double-stranded DNA
  • Degradation of denatured DNA from alkaline-based plasmid purification methods for improved DNA cloning
  • Degradation of contaminating linear and nicked DNA in plasmid samples 

Storage Temperature: -20°C

T5 Exonuclease | NEB酶试剂 New England Biolabs

产品信息

T5 Exonuclease degrades DNA in the 5´ to 3´ direction (1). T5 Exonuclease is able to initiate nucleotide removal from the 5´ termini or at gaps and nicks of linear or circular dsDNA (1). However, the enzyme does not degrade supercoiled dsDNA (2). T5 Exonuclease also has ssDNA endonuclease activity.

产品来源

An E. coli strain that carries a plasmid with the T5 phage D15 gene.

产品类别:
Discontinued Products

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme required to produce 1 nmol of acid soluble deoxyribonucleotide from double-stranded DNA in 30 minutes at 37°C in a total reaction volume of 50 μl.

    反应条件

    1X NEBuffer™ 4
    Incubate at 37°C

    1X NEBuffer™ 4
    50 mM Potassium Acetate
    20 mM Tris-acetate
    10 mM Magnesium Acetate
    1 mM DTT
    (pH 7.9 @ 25°C)

    贮存溶液

    50 mM Tris-HCl
    100 mM NaCl
    1 mM DTT
    0.1 mM EDTA
    50% Glycerol
    0.1% Triton® X-100
    pH 7.5 @ 25°C

    热失活

    否

    单位活性检测条件

    1X NEBuffer 4, 0.15 mM sonicated duplex [3H]-DNA.

  • 优势和特性

    应用特性

    • Degradation of linear ssDNA, dsDNA or nicked plasmid DNA while maintaining supercoiled plasmid DNA.
    • Remove denatured DNA isolated during alkaline lysis-based plasmid purification procedure (5).
    • Enhance the transfection efficiency of miniprep from plasmid cDNA libraries (6).

  • 相关产品

    相关产品

    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

    单独销售的组分

    • NEBuffer 4

  • 参考文献
    1. Sayers, J.R. et al. (1991). Nucleic Acids Res. 19, 4127-4132.
    2. Sayers, J.R. et al. (1990). J. of Biol. Chem. 265, 18311-18317.
    3. Kaliman, A.V. et al. (1986). FEBS. 195, 61-64.
    4. Frenkel, G.D. et al. (1971). J. of Biol. Chem. 246, 4839-4847.
    5. Sayers, J.R. et al. (1996). Analytical Biochem. 241, 186-189.
    6. Kiss-Toth, E. et al. (2001). Analytical Biochem. 288, 230-232.

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for T5 Exonuclease (M0363)

  • 使用指南
    • Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

工具 & 资源

  • 选择指南
    • Activities of Exonucleases and Non-specific Endonucleases
    • Common Applications for Exonucleases and Endonucleases
    • Properties of Exonucleases and Non-specific Endonucleases

  • Web 工具
    • Exo Selector

  • 研究摘要
    • Choosing the Right Exonuclease (2019)

FAQs & 问题解决指南

  • FAQs
    1. How Does T5 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
    2. Will T5 exonuclease cleave supercoiled dsDNA?
    3. How does T5 Exonuclease differ from Exonuclease III (NEB# M0206)?
    4. What is the activity of T5 Exonuclease in the NEBuffers?
    5. Can T5 Exonuclease be heat inactivated?
    6. Can DNA be blunted using T5 Exonuclease?
    7. Will T5 Exonuclease work in rCutSmart buffer?

  • 实验技巧

    Has 5’ to 3’ dsDNA exonuclease activity and ssDNA endonuclease activity.

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    T5 Exonuclease | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

  • 专题文章
    • The effect of nucleic acid modifications on digestion by DNA exonucleases

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0363S_L_v1
    • M0363S_L_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0363L_v1_10011608
    • M0363L_v1_10024696
    • M0363S_v1_10025500
    • M0363L_v1_10034706
    • M0363S_v1_10039595
    • M0363L_v1_10045738
    • M0363S_v1_10045737
    • M0363L_v1_10055613
    • M0363L_v1_10056173
    • M0363L_v1_10057996
    • M0363S_v1_10057226
    • M0363S_v1_10067121

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • T5 Exonuclease