标签归档：enterokinase

Enterokinase, light chain |NEB酶试剂 New England Biolabs

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产品信息

Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.

产品来源

This preparation is purified from Pichia pastoris containing a clone of the light chain of the bovine enterokinase gene.

产品类别:
Proteases Products

应用:
Fusion Protein Cleavage,
Protein Purification,
Protein Digestion

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • P8070S     -20    
        Enterokinase, light chain P8070SVIAL -20 1 x 0.03 ml 16,000 units/ml
    • P8070L     -20    
        Enterokinase, light chain P8070LVIAL -20 1 x 0.16 ml 16,000 units/ml

  • 特性和用法

    单位定义

    1 unit is defined as the amount of enzyme required to cleave 25 µg of a MBP-EK-paramyosin-ΔSal substrate to 95% completion in 16 hours at 25°C in a total reaction volume of 25 µl.

    去除

    Enterokinase will bind specifically to trypsin inhibitor agarose (e.g., Sigma T-0637).

    贮存溶液

    20 mM Tris-HCl
    200 mM NaCl
    2 mM CaCl2
    50% Glycerol
    pH 7.2 @ 4°C

    分子量

    实际: 31 kDa
    理论上的: 26.3 kDa

    单位活性检测条件

    Two fold dilutions of Enterokinase, light chain are incubated with 25 µg MBP-EK-paramyosin-ΔSal substrate in 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2 (pH 8.0 @ 25°C) in a 25 µl reaction. The reaction mix is incubated at 25°C for 16 hours. Separation of reaction products are visualized by SDS-PAGE.

  • 相关产品

    相关产品

    • Furin
    • Factor Xa Protease

  • 注意事项

    1. NaCl concentration above 250 mM will significantly inhibit enzyme activity.
    2. Removal: Enterokinase will bind specifically to trypsin inhibitor agarose (e.g., Sigma T-0637).
    3. Enterokinase is a glycosylated protein.

操作说明、说明书 & 用法

  • 操作说明

    1. Protocol for use with Enterokinase, light chain (P8070)

工具 & 资源

  • 选择指南

    • Protease Selection Chart
    • Substrate-based Ligase Selection Chart