产品信息

• 产品组分信息

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  C2987H     -80       pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl   SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml Not Applicable   NEB® 5-alpha Competent E. coli (High Efficiency) C2987HVIAL -80 20 x 0.05 ml Not Applicable
  C2987I     -80       pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl   SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml Not Applicable   NEB® 5-alpha Competent E. coli (High Efficiency) C2987IVIAL -80 6 x 0.2 ml Not Applicable
  C2987P     -80       pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl   SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml Not Applicable   NEB® 5-alpha Competent E. coli (High Efficiency) C2987PVIAL -80 1 x 96-well plate (20 μl/well) Not Applicable
  C2987R     -80       NEB® 5-alpha Competent E. coli (High Efficiency) C2987RVIAL -80 1 x 384-well plate (10 μl/well) Not Applicable   Outgrowth Medium 1.5 B9040AVIAL 4 1 x 10 ml Not Applicable   pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
  C2987U     -80       SOC Outgrowth Medium B9020SVIAL 4 2 x 25 ml Not Applicable   NEB® 5-alpha Competent E. coli (High Efficiency) C2987UVIAL -80 1 x 96 x 0.05 ml (12 8-well strip) Not Applicable   pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl

• 特性和用法

  Antibiotic for Plasmid Selection

  用于质粒筛选的抗生素	使用浓度
  Ampicillin	100 µg/ml
  Carbenicillin	100 µg/ml
  Chloramphenicol	33 µg/ml
  Kanamycin	30 µg/ml
  Streptomycin	25 µg/ml
  Tetracycline	15 µg/ml

  货运单

  • Ships on dry ice

  抗生素耐药性

  • 无

• 优势和特性

  Features

  • 通用克隆菌株
  • DH5α™ 衍生菌株

  应用特性

  DNA 对转化效率和菌落产量的影响： 

  热激时间对 NEB 5-alpha E.coli 感受态细胞转化效率的影响：

  DNA 温育时间对 NEB 5-alpha E. coli 感受态细胞转化效率的影响：

  细胞复苏培养基对转化效率的影响：

  从 NEB 5-alpha 的高转化效率中获益：

• 相关产品

  相关产品

  • NEB® 5-alpha E. coli 感受态细胞（亚克隆效级）
  • NEB® 5-alpha F’ I^q E. coli 感受态细胞（高效级）
  • t1010-monarch-plasmid-miniprep-kit
  • Monarch® DNA 胶回收试剂盒
  • Monarch® PCR & DNA 纯化试剂盒（5 μg）

  单独销售的组分

  • SOC Outgrowth 培养基

• 注意事项

  1. 针对 C2987H、C2987I、C2987P 和 C2987U 提供 SOC Outgrowth 培养基。
     针对 C2987R 提供 Outgrowth 培养基 1.5。
  2. 注意：本产品含危险物质 DMSO。处理前请先查看 MSDS。
  3. 贮存和处理：
     感受态细胞应在 -80℃ 条件下贮存。-20℃ 条件下贮存会显著降低转化效率。当细胞温度高于 -80℃ 时，即使不融化，细胞也会失去效率。

     SOC 培养基可以在 4℃ 或室温下贮存，具体视使用速度而定。在室温下贮存方便，适合短期内使用贮存（数周到数月）。如需长期贮存，我们建议在 4℃ 条件下贮存。请注意，随 C2987R（1 x 384 孔板包装）提供的 Outgrowth 培养基 1.5 只能在室温下贮存，否则会形成晶体。

操作说明、说明书 & 用法

• 操作说明

  1. 5 Minute Transformation Protocol (C2987H/C2987I)
  2. High Efficiency Transformation Protocol (C2987H/C2987I)
  3. High Efficiency Transformation Protocol for 96-well format (C2987P)
  4. 14 Minute Transformation Protocol (C2987H/C2987I)
  5. High Efficiency Transformation Protocol for 96-tube format (C2987U)
  6. High Efficiency Transformation Protocol for 384-well format (C2987R)

• 使用指南

  • Additional E. coli Strain Genotypes
  • Chemical Transformation Tips
  • Competent E. coli Genome Sequences Tool
  • Genetic Markers
  • McrA, McrBC and EcoKI Strain Phenotypes
  • Restriction of Foreign DNA by E. coli K-12

• 应用实例

  • Enhancing Transformation Efficiency

工具 & 资源

• 选择指南

  • Characteristics of Select E.coli Strains
  • Competent Cell Product Comparison
  • Competent Cell Selection Guide

• Web 工具

  • Competitor Cross-Reference Tool

  • NEBcloner^®

FAQs & 问题解决指南

• FAQs

  1. Which competent cell strains are compatible with Gateway^® Cloning?
  2. Can I store competent cells at -20°C instead of -80°C?
  3. How can I increase transformation efficiency?
  4. How long should I incubate cells on ice after DNA has been added (NEB #C2987H and NEB #C2987I)?
  5. How should I calculate the transformation efficiency (C2987)?
  6. What are the solutions/recipes (C2987)?
  7. What are the strain properties (C2987)?
  8. What is the difference between NEB #C2987H and NEB #C2987I?
  9. What is the difference between NEB #C2988J and NEB #C2987H?
  10. What is the optimal heat shock time for this strain (NEB #C2987H and NEB #C2987I)?
  11. What type of competent cells are suitable for transformation of DNA constructs created using Gibson Assembly?
  12. Which strain of Competent E.coli should I use for general cloning?
  13. Which kind of transformation tubes should be used?
  14. What volume of DNA can be added into competent cells?
  15. Are NEB’s competent cells compatible with the “Mix & Go” protocol?
  16. What is the shelf life for this strain (NEB #C2987H and NEB #C2987I)?
  17. How does the transformation efficiency of the 96-well plate format (NEB #C2987P) compare to the other formats?
  18. What is the optimal heat shock time for the 96-well plate format NEB 5-alpha Competent E.coli (NEB #C2987P)?
  19. I am competing in the iGEM competition. Do you have any products that I should consider from NEB?
  20. What is the optimal heat shock time for the 384-well plate format NEB 5-alpha Competent E. coli (NEB #C2987R)?
  21. What is the optimal heat shock time for the 96-tube format NEB 5-alpha Competent E. coli (NEB #C2987U)?
  22. What type of competent cells are suitable for transformation of DNA constructs created using NEBuilder HiFi DNA Assembly Master Mix?
  23. How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?
  24. How should fragments be prepared for assembly using NEBuilder HiFi?