上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
产品信息
重点
- T7 表达菌株
- Lon 和 OmpT 蛋白酶缺失
- 抗噬菌体 T1（fhuA2）
- B 菌株
- 无动物来源产品
基因型
fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS
λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5
- 产品类别:
- T7 Expression,
- E. coli Protein Expression Strains Products,
- Protein Expression Products,
E. coli Expression Strains Products
- 应用:
- T7 Expression,
- Peptide Ligation,
- Protein Expression in E. Coli,
Protein Expression
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产品组分信息
本产品提供以下试剂或组分：
NEB # 名称 组分货号 储存温度 数量 浓度 -
C2527H -80 SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml Not Applicable pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl BL21(DE3) Competent E. coli C2527HVIAL -80 20 x 0.05 ml Not Applicable
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C2527I -80 SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml Not Applicable pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl BL21(DE3) Competent E. coli C2527IVIAL -80 6 x 0.2 ml Not Applicable
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特性和用法
Antibiotic for Plasmid Selection
用于质粒筛选的抗生素 使用浓度 Ampicillin 100 µg/ml Carbenicillin 100 µg/ml Chloramphenicol 33 µg/ml Kanamycin 30 µg/ml Streptomycin 25 µg/ml Tetracycline 15 µg/ml 货运单
- Ships on dry ice
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优势和特性
Features
- 常规 T7 表达
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相关产品
相关产品
- BL21 E. coli 感受态细胞
- Lemo21（DE3）E. coli 感受态细胞
- NiCo21（DE3）E. coli 感受态细胞
单独销售的组分
- SOC Outgrowth 培养基
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注意事项
- 注意：本产品含有害物质 DMSO。处理前请先查看 MSDS。
- 贮存和处理：感受态细胞应在 -80℃ 下贮存。在 -20℃ 贮存会导致转化效率显著降低。当细胞温度高于 -80℃ 时，即使不融化，细胞也会失去效率。
操作说明、说明书 & 用法
-
操作说明
- 5 Minute Transformation Protocol (C2527)
- Protein Expression Using BL21(DE3) (C2527)
- Transformation Protocol for BL21(DE3) Competent Cells (C2527)
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使用指南
- Additional E. coli Strain Genotypes
- Competent E. coli Genome Sequences Tool
- Genetic Markers
- Making Unmethylated (Dam- Dcm-) DNA
- McrA, McrBC and EcoKI Strain Phenotypes
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应用实例
- Protein Expression with T7 Express Strains
工具 & 资源
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选择指南
- Characteristics of Select E.coli Strains
- Competent Cell Product Comparison
- Competent Cell Selection Guide
FAQs & 问题解决指南
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FAQs
- Does New England Biolabs offer strains of Competent E.coli suited for protein expression?
- What are the strain properties (C2527)?
- Why are there no colonies or no growth in liquid culture (C2527)?
- Why is induced protein insoluble (C2527)?
- Why is there no protein visible on gel or no activity (C2527)?
- What are the solutions/recipes (C2527)?
- Can I store competent cells at -20°C instead of -80°C?
- Which kind of transformation tubes should be used?
- What volume of DNA can be added into competent cells?
- What is the shelf life for this strain (NEB #C2527H and NEB #C2527I)?
- Are NEB’s competent cells compatible with the “Mix & Go” protocol?
- What is the difference between BL21 and BL21(DE3) competent E.coli cells?
- How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?
- Is T7 expression subject to catabolite repression in BL21(DE3) and derivative strains?
