产品信息

• 特性和用法

  单位定义

  One unit is the amount of enzyme required to catalyze the transfer of 1 pmol of methyl group to poly dI.dC substrate in a total reaction volume of 25 μl in 30 minutes at 37°C.

  反应条件

  1X Dnmt1 Reaction Buffer
  Supplement with 100 µg/ml BSA, Molecular Biology Grade 和 160 µM S-adenosylmethionine (SAM)
  Incubate at 37°C

  使用浓度

  2,000 units/ml

  贮存溶液

  50 mM Tris-HCl
  200 mM NaCl
  1 mM DTT
  1 mM EDTA
  50% Glycerol
  pH 7.5 @ 25°C

  热失活

  65°C for 20 minutes

• 相关产品

  单独销售的组分

  • Purified BSA
  • S-腺苷甲硫氨酸（SAM）

• 注意事项

  1. For DNA modification and protection applications, M.SssI (NEB #M0226) is preferred because it efficiently methylates both unmethylated and hemimethylated DNA substrates.

• 参考文献

  1. Pradhan, S. et al. (1999). J. Biol. Chem. . 274, 33002-33010.
  2. Bacolla, A. et al. (1999). J. Biol. Chem. . 274, 33011-33019.
  3. Schmutte, C. et al. (1998). Biol. Chem. . 379, 377-388.
  4. Laird, P.W. et al. (1995). Cell. 81, 197-205.
  5. Li, E. et al. (1992). Cell. 12, 915-926.
  6. Leonhardt, H. et al. (1992). Cell. 71, 865-873.
  7. Yen, R.W. et al. (1992). Nucl. Acids. Res. . 20, 2287-2291.

FAQs & 问题解决指南

• FAQs

  1. Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
  2. What source of tritiated SAM is recommended for use with Dnmt1?
  3. What should be considered if the methylation using Dnmt1 is not going to completion?
  4. How does Dnmt1 differ from SssI methylase?
  5. Can Dnmt1 be heat inactivated?
  6. Will any NEB methyltransferases (methylases) work on RNA?