上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
产品信息
重点
Enhanced BL21 derivative ideal for Plac, Ptac, Ptrc expression vectors
Deficient in proteases Lon and OmpT
Resistant to phage T1 (fhuA2)
Does not restrict methylated DNA (McrA–, McrBC–, EcoBr–m–, Mrr–)
Free of animal products
基因型
fhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10–TetS)2 [dcm] R(zgb-210::Tn10–TetS) endA1 Δ(mcrC-mrr)114::IS10
产品类别:
Non-T7 Expression,
E. coli Protein Expression Strains Products,
Protein Expression Products,
E. coli Expression Strains Products
应用:
Non-T7 Expression,
Protein Expression in E. Coli,
Protein Expression
产品组分信息
本产品提供以下试剂或组分：
NEB #
名称
组分货号
储存温度
数量
浓度
C2523H
-80
SOC Outgrowth Medium
B9020SVIAL
4
1 x 25 ml
Not Applicable
pUC19 Vector
N3041AVIAL
-20
1 x 0.025 ml
50 pg/µl
NEBExpress® Competent E. coli (High Efficiency)
C2523HVIAL
-80
20 x 0.05 ml
Not Applicable
C2523I
-80
SOC Outgrowth Medium
B9020SVIAL
4
1 x 25 ml
Not Applicable
pUC19 Vector
N3041AVIAL
-20
1 x 0.025 ml
50 pg/µl
NEBExpress® Competent E. coli (High Efficiency)
C2523IVIAL
-80
6 x 0.2 ml
Not Applicable
特性和用法
Antibiotic for Plasmid Selection
用于质粒筛选的抗生素
使用浓度
Ampicillin
100 µg/ml
Carbenicillin
100 µg/ml
Chloramphenicol
33 µg/ml
Kanamycin
30 µg/ml
Tetracycline
15 µg/ml
Streptomycin
25 µg/ml
货运单
Ships on dry ice
抗生素耐药性
nit
优势和特性
Features
Versatile non-T7 expression strain
Protease deficient
应用特性
Chemically competent E. coli cells suitable for high efficiency transformation and protein expression. Recommended host strain for pMAL Protein Fusion and Purification System.
DNA Effects on Transformation Efficiency and Colony Output: The optimal amount of DNA to use in a transformation reaction is lower than commonly recognized. Using clean, supercoiled pUC19, the efficiency of transformation is highest in the 100 pg-1 ng range. However, the total colonies which can be obtained from a single transformation reaction increase up to about 100 ng. Effect of heat shock time on NEBExpress competent E. coli transformation efficiency: 50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds. Effect of DNA incubation time on NEB Express competent E. coli transformation efficiency: 50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except DNA incubation time varied from 0 to 40 minutes.
High Efficiency Transformation Protocol (NEB #C2523 and #C3037)
使用指南
Additional E. coli Strain Genotypes
Competent E. coli Genome Sequences Tool
Genetic Markers
Making Unmethylated (Dam- Dcm-) DNA
McrA, McrBC and EcoKI Strain Phenotypes
Restriction of Foreign DNA by E. coli K-12
应用实例
Using the PURExpress®In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening of FMN-dependent Oxidoreductase Variants
Enhancing Transformation Efficiency
工具 & 资源
选择指南
Characteristics of Select E.coli Strains
Competent Cell Selection Guide
FAQs & 问题解决指南
FAQs
Does New England Biolabs offer strains of Competent E.coli suited for protein expression?
How long should I incubate cells on ice after DNA has been added (NEB #C2523H and NEB #C2523I)?
What are the solutions/recipes (C2523)?
What are the strain properties (C2523)?
What is the difference between NEB #C2523H and NEB #C2523I?
Why are there no colonies or no growth in liquid culture (C2523)?
Why is induced protein insoluble (C2523)?
Why is there no protein visible on gel or no activity (C2523)?
Can I store competent cells at -20°C instead of -80°C?
Can the NEBExpress Competent E.coli (High Efficiency) be used for the expression of constructs containing a T7 promoter?
What is the optimal heat shock time for this strain (NEB #C2523H and NEB #C2523I)?
Which kind of transformation tubes should be used?
What volume of DNA can be added into competent cells?
What is the shelf life for this strain (NEB #C2523H and NEB #C2323I)?
Are NEB’s competent cells compatible with the “Mix & Go” protocol?
How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?
Control of IPTG induced expression from Plac, Ptac,Ptrc and PT5-lacO
Protease deficient
应用特性
Chemically competent E. coli cells suitable for high efficiency transformation and protein expression, featuring control of IPTG induced expression with non-T7 plasmids.
DNA Effects on Transformation Efficiency and Colony Output: The optimal amount of DNA to use in a transformation reaction is lower than commonly recognized. Using clean, supercoiled pUC19, the efficiency of transformation is highest in the 100 pg-1 ng range. However, the total colonies which can be obtained from a single transformation reaction increase up to about 100 ng. Effect of heat shock time on NEB Express Iq competent E. coli transformation efficiency: 50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds. Effect of DNA incubation time on NEB Express Iq competent E. coli transformation efficiency: 50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except DNA incubation time varied from 0 to 40 minutes.
