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产品信息
The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in our video library, https://www.neb.com/tools-and-resources/video-library). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes.
- 产品类别:
- Isothermal Amplification & Strand Displacement Products
- 应用:
- Loop-Mediated Isothermal Amplification,
- Isothermal Amplification
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产品组分信息
本产品提供以下试剂或组分：
NEB # 名称 组分货号 储存温度 数量 浓度 -
M1800S -20 WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) M1800SVIAL -20 1 x 1.25 ml 2 X
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M1800L -20 WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) M1800SVIAL -20 5 x 1.25 ml 2 X
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优势和特性
应用特性
- LAMP amplification of RNA and DNA targets
- Simple visual indication of amplification for easy detection and analysis
- One-step field and point-of-need LAMP reactions
- Easy demonstration of LAMP assays with built-in detection
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相关产品
相关产品
- m0538-bst-20-warmstart-dna-polymerase
- m0380-warmstart-rtx-reverse-transcriptase
- 等温扩增缓冲液套装
- dNTP 混合液
- e1700-warmstart-lamp-kit-dna-rna
- m0374-bst-3-0-dna-polymerase
- M1804 WarmStart Colorimetric LAMP 2X Master Mix with UDG
操作说明、说明书 & 用法
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操作说明
- WarmStart Colorimetric LAMP 2X Master Mix Typical LAMP Protocol (M1800)
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应用实例
- Optimized Integration of New England Biolabs® Loop-mediated Isothermal Amplification (LAMP) Reagents with Axxin ISO Instruments
- Facilitating Detection of SARS-CoV-2 Directly from Patient Samples: Precursor Studies with RT-qPCR and Colorimetric RT-LAMP Reagents
工具 & 资源
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Web 工具
- NEB LAMP Primer Design Tool
FAQs & 问题解决指南
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FAQs
- What is the difference between using Colorimetric LAMP and regular LAMP reactions?
- How long should I incubate the Colorimetric LAMP reaction before checking the results?
- What components are contained in the WarmStart® Colorimetric LAMP 2X Master Mix and what else do I need to perform a LAMP reaction?
- What is the Mg++ concentration in the Colorimetric LAMP 2X Master Mix?
- Can the Colorimetric LAMP 2X Master Mix be used in instruments that utilize fluorescent detection of DNA amplification or real time turbidity measurement?
- How do I design LAMP Primers?
- The WarmStart LAMP Master Mix has some precipitation in the tube after thawing, is this normal?
- How stable are the WarmStart Colorimetric LAMP 2X Master Mixes?
- What buffer should I use for my target samples and primers going into the colorimetric LAMP reaction?
- What components are contained in the WarmStart Colorimetric LAMP Master Mix and what else I need to perform a LAMP reaction?
- What is the difference between the WarmStart® Colorimetric LAMP 2X Master Mix with UDG (NEB #M1804) and the WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB #M1800)?
- What if my colorimetric LAMP mix is orange instead of pink prior to amplification?
- Should I use Guanidine HCl (B2619A) in colorimetric LAMP reactions?
- How much sample can be added to the colorimetric LAMP reaction as input?
- Can I monitor the color change of the colorimetric LAMP reactions using a spectrophotometer?
- Can the WarmStart Colorimetric LAMP 2X Master Mixes be used in instruments that utilize fluorescence detection?
- What is the optimal LAMP/RT-LAMP amplification temperature?
- How fast should I expect a result?
- Can I set up my LAMP/RT-LAMP reactions at room temperature?
- Amplification occurred in my NTC sample(s) following isothermal incubation. What happened?
- Is a separate incubation step required for RT-LAMP?
- Does NEB have a master mix for LAMP or RT-LAMP reactions?
- What is LAMP and RT-LAMP?
- What type of purification is recommended for LAMP primers?