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脱脂奶粉

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  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

脱脂奶粉脱脂奶粉


Blotting实验专用

制造商：FUJIFILM Wako Pure Chemical Corporation（富士胶片和光）

储存条件：室温脱脂奶粉


◆概况

脱脂奶粉，脂肪含量低，蛋白含量高。配制成0.3~5%的水溶液使用。



用途


作为抗体反应等的印迹封闭溶液使用，也可用于干燥标本的巴氏染色前处理。



注意点


当一抗为抗磷酸化抗体时，脱脂奶粉由于含有较多磷酸化蛋白，请改为使用BSA。

◆应用实例


<Western Blotting应用实例1>

脱脂奶粉

　

低背景！

1.　1 w/v% Skim milk in TBS-T

2.　5 w/v% BSA(Albumin, from Bovine Serum) in TBS-T

上样量：10 ug of cell lysate/Lane

抗体：Anti β-actin antibody

<Western Blotting应用实例1>


脱脂奶粉

低背景！

1.　5w/v% Skim milk in TBS-T

2.　Company A Protein-free Blocking Buffer

样品：DYKDDDDK-BAP

Lane#1： 10 ng

Lane#2： 5 ng

Lane#3： 2.5 ng

Antibody : Anti DYKDDDDK antiboby


◆物性信息

外观

白色~淡黄色，粉末



溶解性


在水中变浑浊然后溶解。


※ 本页面产品仅供研究用，研究以外不可使用。


产品编号 产品名称 产品规格 产品等级
190-12865 Skim Milk Powder
脱脂奶粉		 500 g

Trypsin-digested BSA MS Standard (CAM-modified) |NEB酶试剂 New England Biolabs

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上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

A complex mixture of peptides produced by Trypsin digestion of Bovine Serum Albumin (BSA) that was reduced and alkylated with Iodoacetamide (CAM modified). This peptide mixture can be used to test a Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF) or Electrospray Ionization (ESI) mass spectrometer (TOF, Q-TOF or Ion Trap).

MALDI Analysis of BSA Digest:
Trypsin-digested BSA MS Standard (CAM-modified) |
The BSA digest is dissolved to 1 pmol/μl. One µl of digest is mixed with 1 µl of α-cyano-4-hydroxycinnamic acid (10 mg/ml in 50:50 acetonitrile:water with 0.1% trifluoroacetic acid). One μl of the digest/matrix solution were then spotted directly onto a MALDI target plate. 
Online Analysis of BSA Digest:
Trypsin-digested BSA MS Standard (CAM-modified) |
The BSA digest solution was diluted to 100 fmol/μl with 0.1% formic acid. One μl (100 fmol) of the digest solution was injected via an Easy-nanoLC II (Proxeon) onto a self-packed reverse phase C18 nano column (Phenomenex packing material, 5 cm, 150 μm) and loaded onto a self-packed C18 analytical column with an integrated tip (New Objective Picofrit, 15 cm, 100 μm, Phenomenex packing material). Peptides were separated using a 45 min 5-70%B gradient (A = 0.1% formic acid, B = CH3CN, 0.1% formic acid) at a flow rate of 400 nl/min. Eluting peptides were analyzed online by a Q-Exactive mass spectrometer (Thermo Scientific) with an electrospray ion source. MS/MS spectra were acquired by HCD using an acquisition range of 400 to 1600 m/z.

产品来源

BSA (GENBANK P02769) was digested using Trypsin (TPCK-treated).

重组

Suggested volume to resuspend: 500 µl. Avoid repeated freeze/thaw cycles once in solution.

产品类别:
Proteome Analysis Products

应用:
Proteomics

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • P8108S     -20    
        Trypsin-digested BSA MS Standard (CAM-modified) P8108SVIAL -20 1 x 500 pmol Not Applicable

  • 注意事项

    1. Supplied in dry format.
    2. This product has lot-to-lot variations in peptide frequency and abundance.

BSA，分子生物学级 |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

部分产品随附牛血清白蛋白（BSA）， 可以用于防止酶粘附到 反应管壁上及移液枪 表面。BSA 还可以用于在温育期间 稳定某些蛋白质。

产品类别:
Discontinued (<3 years)

  • 特性和用法

    贮存溶液

    20 mM Tris-HCl
    100 mM KCl
    0.1 mM EDTA
    50% Glycerol
    pH 8 @ 25°C

  • 相关产品

    相关产品

    • t1010-monarch-plasmid-miniprep-kit
    • Monarch® DNA 胶回收试剂盒
    • Monarch® PCR & DNA 纯化试剂盒（5 μg）
    • 重组白蛋白, 分子生物学级（不含动物成分）

  • 参考文献

    1. Pace, C.N. et al. (1995). Protein Sci,. 4, 2411-2423.

FAQs & 问题解决指南

  • FAQs

    1. What is the source of your BSA?
    2. What is the buffer composition of your BSA?
    3. Why do I need to add BSA to my reaction?
    4. Is the BSA in its native form?
    5. Can I use your BSA as a molecular weight marker?
    6. What is the difference between BSA (B9001S) which was discontinued, versus BSA, Molecular Biology Grade (B9000S)?
    7. Is the BSA acetylated?

Albumin, from Bovine Serum (BSA), Crystallized 牛血清白蛋白结晶

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Albumin, from Bovine Serum (BSA), Crystallized

牛血清白蛋白结晶

品牌：FUJIFILM Wako
CAS No.：9048-46-8
储存条件：2-10℃
纯度：95.0+% (SDS-PAGE)
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

016-15091

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