上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
产品信息
产品来源
An E. coli strain that carries human Fbs1 mutant gene.
- 产品类别:
- Glycobiology Products,
- Proteome Analysis Products
- 应用:
- Glycobiology & Proteomics,
- Glycoprotein Analysis
-
产品组分信息
本产品提供以下试剂或组分：
NEB # 名称 组分货号 储存温度 数量 浓度 -
P0872S -20 N-Glycopeptide Binding Protein P0872SVIAL -20 1 x 1 ml 1 mg/ml
-
-
特性和用法
贮存溶液
20 mM Tris-HCl
50 mM NaCl
0.2 mM TCEP
pH 8热失活
否
分子量
理论上的: 45 kDa
单位活性检测条件
Functional Testing (N–Glycan Enrichment) – A 200 μL reaction in 20 mM Tris-HCl, 50 mM NaCl, 0.2 mM TCEP, 10% acetonitrile, pH 8.0 containing 1 nmol of fluorescently labeled sialylglycopeptide and 100 μg N–Glycopeptide Binding Protein incubated for 30 minutes at 4°C results in ≥40% enrichment as determined by UV-Vis Spectroscopy at 520 nm.
-
优势和特性
Features
- Engineered lectin, a glycan-binding protein that recognizes a wide variety of N-glycoforms, for enrichment and identification of glycopeptides and glycoproteins
- Selectively binds and enriches N-glycopeptides in a complex proteomics sample, unlike other lectin affinity chromatography (LAC) techniques, HILIC, or other lectin-based enrichment methods
- Significantly increased glycopeptide spectrum match and glycoprotein ID and discovery
- Versatile workflow
-
相关产品
相关产品
- Trypsin-ultra™, Mass Spectrometry Grade
- Endoproteinase LysC
- SNAP-捕获磁珠
- 6 孔磁性分离架
-
参考文献
- Chen M. et al. (2017). An engineered high affinity Fbs1 carbohydrate binding protein for selective capture of N-glycans and N-glycopeptides. Nat Commun. May 23;8, 15487.
- Chen M., et al. (2023). Comparative site-specific N-glycoproteome analysis reveals aberrant N-glycosylation and gives insights into mannose-6-phosphate pathway in cancer. Commun Biol. Jan13;6(1), 48.
- Mersha F. et al. (2023). Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malay. Sci Rep. May 16;13(1), 7951.
操作说明、说明书 & 用法
-
操作说明
- N-Glycopeptide Binding Protein Typical Reaction Protocol (NEB #P8072)
FAQs & 问题解决指南
-
FAQs
- How does N-Glycopeptide Binding Protein improve glycoproteomic analysis?
- What is the specificity of N-Glycopeptide Binding Protein?
- How strong does the N–Glycopeptide Binding Protein bind to a glycopeptide?
- Can N-Glycopeptide Binding Protein bind and enrich free glycans?
- Can N-Glycopeptide Binding Protein bind an intact glycoprotein?
- What influences the binding affinity of the N-Glycopeptide Binding Protein?