产品信息

Nuclease BAL-31 exonuclease degrades both 3′ and 5′ termini of duplex DNA without generating internal scissions. The enzyme is also a highly specific single-stranded endonuclease which cleaves at nicks, gaps and single-stranded regions of duplex DNA and RNA (1,2).

Ligation of Nuclease BAL-31 treated fragments 

产品来源

Purified from the culture medium of Alteromonas espejiana BAL-31. Contains a mixture of “fast” and “slow” species of the enzyme (3).

产品类别:

Discontinued (<3 years)

• 特性和用法

  单位定义

  One unit is defined as the amount of enzyme required to remove 200 base pairs from each end of linearized double-stranded ΦX174 DNA (40 µg/ml) in a total reaction volume of 50 μl in 10 minutes at 30°C in 1X Nuclease BAL-31 Reaction Buffer.

  反应条件

  1X Nuclease BAL-31 Reaction Buffer
  Incubate at 30°C

  1X Nuclease BAL-31 Reaction Buffer
  20 mM Tris-HCl
  600 mM NaCl
  12 mM MgCl₂
  12 mM CaCl₂
  1 mM EDTA
  (pH 8 @ 25°C)

  贮存溶液

  0.25 mM EDTA
  10 mM Tris-HCl
  50 mM NaCl
  1.5 mM CaCl₂
  1.5 mM MgCl₂
  200 µg/ml BSA
  50% Glycerol
  pH 8 @ 25°C

  热失活

  65°C for 20 minutes

  in the presence of 30mM EGTA

• 优势和特性

  应用特性

  • Progressive shortening of double-stranded DNA fragments at both termini (4)
  • Restriction site mapping (2)

• 相关产品

  相关产品

  • t1010-monarch-plasmid-miniprep-kit
  • Monarch® DNA 胶回收试剂盒
  • Monarch® PCR & DNA 纯化试剂盒（5 μg）

• 注意事项

  1. Duplex products of the exonuclease are a mixture of blunt and staggered ends. This mixture can be cloned directly, although maximal ligation efficiency requires repairing the staggered ends with a suitable DNA polymerase.
  2. If necessary, the enzyme may be diluted in reaction buffer prior to use.
  3. Activity is linear with enzyme concentration.
  4. Heat Inactivation will only work in the presence of 30mM EGTA.

• 参考文献

  1. Gray, H.B. et al. (1975). Nucl.Acids Res.. 2, 1459-1492.
  2. Legerski, R.J. et al. (1978). Nucl.Acids Res.. 5, 1445-1463.
  3. Wei, C.-F. et al. (1983). J. Biol.Chem.. 258, 13506-13512.
  4. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual(2nd Ed.). 5.73-5.75.

操作说明、说明书 & 用法

• 操作说明

  1. Protocol for Nuclease BAL-31

工具 & 资源

• Web 工具

  • Exo Selector

FAQs & 问题解决指南

• FAQs

  1. Can Nuclease BAL-31 be used to remove 10 base pairs from the end of a DNA fragment?
  2. Why does all of the DNA get degraded when I use Nuclease BAL-31?
  3. Can Nuclease BAL-31 be heat inactivated?
  4. Is Nuclease BAL-31 active in other NEBuffers?
  5. Can Nuclease BAL-31 treated DNA be cloned?
  6. What is a good control for the BAL-31 nuclease?
  7. Will Nuclease BAL-31 degrade RNA?

Nuclease BAL-31 exonuclease degrades both 3′ and 5′ termini of duplex DNA without generating internal scissions. The enzyme is also a highly specific single-stranded endonuclease which cleaves at nicks, gaps and single-stranded regions of duplex DNA and RNA (1,2).

Ligation of Nuclease BAL-31 treated fragments 

产品来源

Purified from the culture medium of Alteromonas espejiana BAL-31. Contains a mixture of “fast” and “slow” species of the enzyme (3).

产品类别:

Discontinued Products

• 特性和用法

  单位定义

  One unit is defined as the amount of enzyme required to remove 200 base pairs from each end of linearized double-stranded ΦX174 DNA (40 µg/ml) in a total reaction volume of 50 μl in 10 minutes at 30°C in 1X Nuclease BAL-31 Reaction Buffer.

  反应条件

  1X Nuclease BAL-31 Reaction Buffer
  Incubate at 30°C

  1X Nuclease BAL-31 Reaction Buffer
  20 mM Tris-HCl
  600 mM NaCl
  12 mM MgCl₂
  12 mM CaCl₂
  1 mM EDTA
  (pH 8 @ 25°C)

  贮存溶液

  0.25 mM EDTA
  10 mM Tris-HCl
  50 mM NaCl
  1.5 mM CaCl₂
  1.5 mM MgCl₂
  200 µg/ml BSA
  50% Glycerol
  pH 8 @ 25°C

  热失活

  65°C for 20 min

  in the presence of 30mM EGTA

• 优势和特性

  应用特性

  • Progressive shortening of double-stranded DNA fragments at both termini (4)
  • Restriction site mapping (2)

• 相关产品

  相关产品

  • Monarch® Plasmid Miniprep Kit 
  • Monarch® DNA Gel Extraction Kit
  • Monarch® PCR & DNA Cleanup Kit (5 μg)

• 注意事项
  1. Duplex products of the exonuclease are a mixture of blunt and staggered ends. This mixture can be cloned directly, although maximal ligation efficiency requires repairing the staggered ends with a suitable DNA polymerase.
  2. If necessary, the enzyme may be diluted in reaction buffer prior to use.
  3. Activity is linear with enzyme concentration.
  4. Heat Inactivation will only work in the presence of 30mM EGTA.

• 参考文献
  1. Gray, H.B. et al. (1975). Nucl.Acids Res.. 2, 1459-1492.
  2. Legerski, R.J. et al. (1978). Nucl.Acids Res.. 5, 1445-1463.
  3. Wei, C.-F. et al. (1983). J. Biol.Chem.. 258, 13506-13512.
  4. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual(2nd Ed.). 5.73-5.75.

• 操作说明
  1. Protocol for Nuclease BAL-31

• Web 工具
  • Exo Selector

• FAQs
  1. Can Nuclease BAL-31 be used to remove 10 base pairs from the end of a DNA fragment?
  2. Why does all of the DNA get degraded when I use Nuclease BAL-31?
  3. Can Nuclease BAL-31 be heat inactivated?
  4. Is Nuclease BAL-31 active in other NEBuffers?
  5. Can Nuclease BAL-31 treated DNA be cloned?
  6. What is a good control for the BAL-31 nuclease?
  7. Will Nuclease BAL-31 degrade RNA?

• 引用文献
  产品引用文献查找工具

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• 质控分析
  每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

• 产品说明与变更通知

  产品说明与变更通知

  产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

  • M0213S_L_v1

• CoA
  CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

  • M0213S_v0_10016795
  • M0213S_v0_10034567
  • M0213S_v1_10036382
  • M0213S_v1_10049151
  • M0213S_v1_10084119
  • M0213S_v1_10109043

• SDS
  以下 SDS 文件可以帮助您安全地使用该产品

  • Nuclease BAL-31