上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
产品信息
An affinity matrix used for the isolation of proteins fused to maltose-binding protein. It is intended for use in a gravity flow column.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
- 产品类别:
- Amylose Purification (MBP-tag),
- Affinity Purification Products,
- Protein Purification Products
- 应用:
- MBP Affinity Tag,
- Affinity Purification & Expression Tags
-
产品组分信息
本产品提供以下试剂或组分：
NEB # 名称 组分货号 储存温度 数量 浓度 -
E8021V 4 Amylose Resin E8021VVIAL 4 1 x 10 ml Not Applicable
-
E8021S 4 Amylose Resin E8021SVIAL 4 1 x 15 ml Not Applicable
-
E8021L 4 Amylose Resin E8021LVIAL 4 1 x 100 ml Not Applicable
-
-
特性和用法
贮存溶液
20% Ethanol
结合容量
>4mg MBP5*-paramyosin ΔSal fusion protein/ml amylose resin.
可再生
Yes
-
注意事项
- Store at 4°C. After use, resin should be stored incolumn buffer plus 0.02% sodium azide or 20% ethanol.
- Amylose Resin column should be washed with 5 volumes ofcolumn buffer before each use.
- For optimum performance, load crude extract at < 60cm/hour.
- When regenerating the column at 4°C, please note that0.1% SDS can precipitate at that temperature. It is therefore recommended thatthe SDS solution be stored at room temperature until needed. The resin may begenerated up to five times.
- For a complete affinity purification protocol, download the pMAL Protein Fusion and Purification System technical bulletin (NEB #E8200) from www.neb.com.
- Regeneration: The packed resin may beregenerated by the following wash sequence: Water 3 – column volumes, 0.1% SDS -3 column volumes, Water – 1 column volume, Column Buffer – 5 columnvolumes.
工具 & 资源
-
选择指南
- Purification Beads, Columns and Resins
FAQs & 问题解决指南
-
FAQs
- Much of my fusion protein flows through the amylose column. Is there anything I can do to improve my fusion’s affinity for the amylose column?
- How many times can I use the amylose column?
- What is known about binding in the presence of nonionic detergents?
- Can I substitute a different buffer and/or salt concentration in the Column Buffer?
- I see my intact fusion protein by SDS-PAGE when I run cells boiled in Sample Buffer, but when I check the crude extract the fusion is degraded.
- When I run my purified fusion protein on SDS-PAGE, why do I see multiple bands instead of a single band of the expected MW?
- Can I perform a batch purification using the amylose resin?
- Can MBP fusions be purified in the presence of denaturants like urea or guanidine-HCl?
- Is the amylose resin damaged by storage at -20°C?
- What gravity column do you recommend?
- Can amylose resin be cleaned, stored, and used again?