上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
产品信息
96 次反应的试剂盒含有一板一次性的密封着可穿刺的铝箔封板膜 96 孔板。96 孔板的每个孔都含有一对唯一的 i5 和 i7 Index 引物，因此共提供 96 种 Unique 双端 Index 引物。对于 24 次反应的试剂盒，Index 引物封装在 24 个单独的小管中，每个小管都含有一对独特的 i5 和 i7 Index 引物。24 次和 96 次反应试剂盒中提供的 i5 和 i7 Index 引物之间没有重复。
虽然 NEBNext EM-seq 接头针对 EM-seq 实验流程进行了优化，但同样适用于重亚硫酸盐测序实验流程。
NEBNext 酶学转化法甲基化建库试剂盒（NEB #E7120）也提供这些组份。
- 产品类别:
- NEBNext® Multiplex Oligos (Adaptors & Primers) Products,
- Epigenetic Analysis Products,
- Epigenetics Products,
Next Generation Sequencing Library Preparation Products
- 应用:
- DNA Methylation Analysis,
- Bisulfite Conversion,
- Epigenetics
-
试剂盒组成
本产品提供以下试剂或组分：
NEB # 名称 组分货号 储存温度 数量 浓度 -
E7140S -20 EM-seq™ Index Primer 1 (S) E7141AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 2 (S) E7142AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 3 (S) E7143AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 4 (S) E7144AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 5 (S) E7145AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 6 (S) E7146AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 7 (S) E7147AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 8 (S) E7148AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 9 (S) E7149AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 10 (S) E7150AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 11 (S) E7151AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 12 (S) E7152AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 13 (S) E7153AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 14 (S) E7154AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 15 (S) E7155AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 16 (S) E7156AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 17 (S) E7157AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 18 (S) E7158AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 19 (S) E7159AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 20 (S) E7160AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 21 (S) E7161AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 22 (S) E7162AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 23 (S) E7163AVIAL -20 1 x 0.005 ml 10 µM EM-seq™ Index Primer 24 (S) E7164AVIAL -20 1 x 0.005 ml 10 µM NEBNext® EM-seq™ Adaptor E7165AVIAL -20 1 x 0.06 ml Not Applicable
-
E7140L -20 NEBNext® 96 Unique Dual Index Primer Pairs Plate (L) E7166AVIAL -20 1 x 5 μl/well 10 µM NEBNext® EM-seq™ Adaptor E7165AAVIAL -20 1 x 0.24 ml Not Applicable
-
操作说明、说明书 & 用法
-
操作说明
- Where can I find protocols for using the NEBNext® Enzymatic Methyl-seq Kit? (NEB #E7120, E7125, E7140)
-
说明书
产品说明书包含产品使用的详细信息、产品配方和质控分析。- manualE7140
-
使用指南
- E7140L_E7120L_EMSeqUDI_IlluminaLRM
- E7140S_E7120S_EMSeqUDI_IlluminaLRM
- Forward Strand Workflow Sample Sheet for E7140L: NovaSeq 6000 with v1.0 reagent kits, MiniSeq with Rapid reagent kits, MiSeq, HiSeq 2000/2500 (paired-end flow cell), HiSeq 3000/4000 (single-read flow cell)
- Forward Strand Workflow Sample Sheet for E7140S: NovaSeq 6000 with v1.0 reagent kits, MiniSeq with Rapid reagent kits, MiSeq, HiSeq 2000/2500 (paired-end flow cell), HiSeq 3000/4000 (single-read flow cell)
- Reverse Complement Workflow Sample Sheet for E7140L: iSeq 100, MiniSeq with Standard reagent kits, NextSeq Systems, NovaSeq 6000 with v1.5 reagent kits, HiSeq 2000/2500 (single-read flow cell), HiSeq 3000/4000 (paired-end flow cell)
- Reverse Complement Workflow Sample Sheet for E7140S: iSeq 100, MiniSeq with Standard reagent kits, NextSeq Systems, NovaSeq 6000 with v1.5 reagent kits, HiSeq 2000/2500 (single-read flow cell), HiSeq 3000/4000 (paired-end flow cell)
工具 & 资源
-
选择指南
- NEBNext® Multiplex Oligos Selection Chart
FAQs & 问题解决指南
-
FAQs
- What is provided in the NEBNext Multiplex Oligos for Enzymatic Methyl-seq?
- Can the NEBNext® Multiplex Oligos for Enzymatic Methyl-seq be used to address “index hopping” with Illumina patterned flow cells?
- How many indices are available with the 24 reaction- or 96 reaction-size of the NEBNext® Multiplex Oligos for Enzymatic Methyl-seq?
- Are the NEBNext® Multiplex Oligos for Enzymatic Methyl-seq the same as NEBNext® Multiplex Oligos for Illumina®?
- Are the EM-seq Adaptor and Index Primers validated in the EM-seq workflow?
- What is the concentration of the EM-seq Adaptor and Index Primers?
- How many reactions worth of primer are in each well/tube?
- Can I use the 96-well primer plate to do my PCR reactions?
- Do I need to spike in custom sequencing primers when sequencing libraries made with NEBNext® Multiplex Oligos for Enzymatic Methyl-seq on Illumina sequencing instruments?
- Can I perform single read runs and still get both index sequences? Can I run both single read and paired-end recipes with dual-indexed libraries?
- Are Sample Sheets available for use with the NEBNext® Multiplex Oligos for EM-seq?
- Can the EM-seq Adaptor be used for bisulfite sequencing?
- Are dual indexed libraries compatible with single end sequencing?
- What sequences need to be trimmed for NEBNext libraries that are sequenced on an Illumina instrument?
