产品信息

• 试剂盒组成

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  E6448S     -20       NEBNext® Adaptor for Illumina® E6612AVIAL -20 1 x 0.96 ml 15 µM   USER® Enzyme E6610AVIAL -20 1 x 0.288 ml Not Applicable   NEBNext® Unique Dual Index Primer Set 5 E6449AVIAL -20 1 x 10 μl/well 10 µM
  E6448L     -20       NEBNext® Adaptor for Illumina® E6612AVIAL -20 4 x 0.96 ml 15 µM   USER® Enzyme E6610AAVIAL -20 2 x 0.576 ml Not Applicable   NEBNext® Unique Dual Index Primer Set 5 E6449AVIAL -20 4 x 10 μl/well 10 µM

• 相关产品

  相关产品

  • NEBNext^® 多样本接头引物试剂盒 1（96 种 Unique 双端 Index 引物）
  • NEBNext^® 多样本接头引物试剂盒 1（96 种 Unique 双端 Index 引物）
  • NEBNext^® 多样本接头引物试剂盒 2（96 种 Unique 双端 Index 引物）
  • NEBNext^® 多样本接头引物试剂盒 2（96 种 Unique 双端 Index 引物）
  • NEBNext^® Multiplex Oligos for Illumina^® (96 Unique Dual Index Primer Pairs Set 3)
  • NEBNext^® Multiplex Oligos for Illumina^® (96 Unique Dual Index Primer Pairs Set 3)
  • NEBNext^® 多样本接头引物试剂盒 4（96 x 96 种 Unique 匹配双端 Index 引物）
  • NEBNext^® 多样本接头引物试剂盒 4（96 x 96 种 Unique 匹配双端 Index 引物）

操作说明、说明书 & 用法

• 操作说明

  1. Where can I find protocols for using NEBNext^® Multiplex Oligos for Illumina^® (96 Unique Dual Index Primer Pairs)? (NEB #E6440, #E6442, #E6444, #E6446, #E6448)

• 说明书

  产品说明书包含产品使用的详细信息、产品配方和质控分析。

  • manualE6448

• 使用指南

  • E6448_UDI_IlluminaLRM
  • Forward Strand Workflow Sample Sheet for E6448: NovaSeq 6000 with v1.0 reagent kits, MiniSeq with Rapid reagent kits, MiSeq, HiSeq 2000/2500 (paired-end flow cell), HiSeq 3000/4000 (single-read flow cell)
  • Reverse Complement Workflow Sample Sheet for E6448: iSeq 100, MiniSeq with Standard reagent kits, NextSeq Systems, NovaSeq 6000 with v1.5 reagent kits, HiSeq 2000/2500 (single-read flow cell), HiSeq 3000/4000 (paired-end flow cell)

工具 & 资源

• 选择指南

  • NEBNext® Multiplex Oligos Selection Chart

FAQs & 问题解决指南

• FAQs

  1. Are NEBNext adaptors and primers for Illumina validated in Next Generation Sequencing Workflows?
  2. What is the concentration of the adaptor and primers in the NEBNext Multiplex Oligos kits?
  3. Can I use the 96-well primer plate to do my PCR reactions?
  4. How many reactions worth of adaptor is in each well?
  5. Is the NEBNext adaptor methylated?
  6. Can I perform single read runs and still get both index sequences? Can I run both single read and paired-end recipes with dual-indexed libraries?
  7. Are dual indexed libraries compatible with single end sequencing?
  8. How many indices are available with NEBNext^® Multiplex Oligos for Illumina^® (96 Unique Dual Index Primer Pairs) (NEB #E6440, E6442, E6444, E6446, and E6448)?
  9. Can the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB #E6440, #E6442, #E6444, #E6446, and #E6448) be used to address “index hopping” with Illumina patterned flow cells?
  10. What should I do if the sample sheet from the NEB.com website is not compatible with the software version I am using?
  11. Which sets of NEBNext Unique Dual Index Primer Pairs can be combined in a single Illumina sequencing run?
  12. What sequences need to be trimmed for NEBNext libraries that are sequenced on an Illumina instrument?

产品信息

NEBNext^® 接头采用了一种新型的发卡环状结构，能以更高的效率连接末端经修复和加 dA 尾的 DNA 片段。环状结构 DNA 中包含一个 U，当 U 被 USER™ 酶（UDG 和 Endo VIII 的混合物）切掉后，环状结构打开，使它可以成为 PCR 反应的底物。通过 NEBNext Index 引物在 PCR 步骤中引入 Barcode，从而有助于实现多重测序文库制备。该试剂盒包含 8 种 i5 Index 引物和 12 种 i7 Index 引物。

NEBNext 接头引物可与 NEBNext 产品以及其它兼容 Illumina 平台的标准文库制备流程结合使用。

还提供 12 种和 96 种单端 Index 引物（NEB #E7335、 NEB #E7500、NEB #E7710、NEB #E7730、NEB #E6609）。也提供甲基化的 NEBNext 接头（NEB #E7535），可用于重亚硫酸盐测序流程。

双端 Index 引物实验流程

批次质控

所提供的单独批次均通过额外功能验证。单个试剂通过标准酶活性和质控分析，并同时满足各单独组分页面列出的严格的附加质控标准

产品类别:

NEBNext® Multiplex Oligos (Adaptors & Primers) Products

应用:

Applications of USER® and Thermolabile USER II Enzymes

• 试剂盒组成

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  E7600S     -20       NEBNext Adaptor for Illumina E7601AVIAL -20 1 x 0.96 ml Not Applicable   USER® Enzyme E7602AVIAL -20 1 x 0.288 ml Not Applicable   NEBNext i501 Primer E7603AVIAL -20 1 x 0.06 ml 10 µM   NEBNext i502 Primer E7604AVIAL -20 1 x 0.06 ml 10 µM   NEBNext i503 Primer E7605AVIAL -20 1 x 0.06 ml 10 µM   NEBNext i504 Primer E7606AVIAL -20 1 x 0.06 ml 10 µM   NEBNext i505 Primer E7607AVIAL -20 1 x 0.06 ml 10 µM   NEBNext i506 Primer E7608AVIAL -20 1 x 0.06 ml 10 µM   NEBNext i507 Primer E7609AVIAL -20 1 x 0.06 ml 10 µM   NEBNext i508 Primer E7610AVIAL -20 1 x 0.06 ml 10 µM   NEBNext i701 Primer E7611AVIAL -20 1 x 0.04 ml 10 µM   NEBNext i702 Primer E7612AVIAL -20 1 x 0.04 ml 10 µM   NEBNext i703 Primer E7613AVIAL -20 1 x 0.04 ml 10 µM   NEBNext i704 Primer E7614AVIAL -20 1 x 0.04 ml 10 µM   NEBNext i705 Primer E7615AVIAL -20 1 x 0.04 ml 10 µM   NEBNext i706 Primer E7616AVIAL -20 1 x 0.04 ml 10 µM   NEBNext i707 Primer E7617AVIAL -20 1 x 0.04 ml 10 µM   NEBNext i708 Primer E7618AVIAL -20 1 x 0.04 ml 10 µM   NEBNext i709 Primer E7619AVIAL -20 1 x 0.04 ml 10 µM   NEBNext i710 Primer E7620AVIAL -20 1 x 0.04 ml 10 µM   NEBNext i711 Primer E7621AVIAL -20 1 x 0.04 ml 10 µM   NEBNext i712 Primer E7622AVIAL -20 1 x 0.04 ml 10 µM

• 特性和用法

  需要但不提供的材料

  有关其它所需材料，请查看 NEBNext 文库制备试剂盒的手册。

• 相关产品

  相关产品

  • e7760-nebnext-ultra-ii-directional-rna-library-prep-kit-for-illumina
  • e7765-nebnext-ultra-ii-directional-rna-library-prep-with-sample-purification-beads
  • NEBNext^® Ultra^™ II RNA 非链特异性文库制备试剂盒
  • e7775-nebnext-ultra-ii-rna-library-prep-with-sample-purification-beads
  • NEBNext® Ultra™ II FS DNA 文库制备试剂盒 – 含片段化酶
  • NEBNext® Ultra™ II FS DNA 文库制备试剂盒 – 含片段化酶（含纯化磁珠）
  • NEBNext^® Ultra™ II DNA 文库制备试剂盒
  • NEBNext^® Ultra^™ II DNA 文库制备试剂盒（含纯化磁珠）
  • NEBNext® Ultra™ II 连接模块
  • NEBNext® Ultra™ DNA 文库制备试剂盒
  • NEBNext® Ultra 连接模块
  • NEBNext® 快速连接模块

操作说明、说明书 & 用法

• 操作说明

  1. Setting up the PCR Reaction – NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (E7600)

• 说明书

  产品说明书包含产品使用的详细信息、产品配方和质控分析。

  • manualE7600

• 使用指南

  • E7600_DualIndex_IlluminaLRM
  • Forward Strand Workflow Sample Sheet for E7600: NovaSeq 6000 with v1.0 reagent kits, MiniSeq with Rapid reagent kits, MiSeq, HiSeq 2000/2500 (paired-end flow cell), HiSeq 3000/4000 (single-read flow cell)
  • Reverse Complement Workflow Sample Sheet for E7600: iSeq 100, MiniSeq with Standard reagent kits, NextSeq Systems, NovaSeq 6000 with v1.5 reagent kits, HiSeq 2000/2500 (single-read flow cell), HiSeq 3000/4000 (paired-end flow cell)

工具 & 资源

• 选择指南

  • NEBNext® Multiplex Oligos Selection Chart

FAQs & 问题解决指南

• FAQs

  1. Where can I find the sample sheets for these Multiplex Oligos?
  2. Do NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1)come with library prep reagents?
  3. Can I use NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) for library prep from both DNA and RNA samples?
  4. How many libraries can I prepare with the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1)?
  5. For pooling <96 samples, what combination of indices should I use?
  6. Can I perform single read runs and still get both index sequences? Can I run both single read and paired-end recipes with dual-indexed libraries?
  7. Are dual indexed libraries compatible with single end sequencing?
  8. Is the USER enzyme in our NEBNext kits identical in concentration to the standalone product?
  9. How do I use the NEB provided Sample Sheet for my specific instrument?
  10. What sequences need to be trimmed for NEBNext libraries that are sequenced on an Illumina instrument?

产品信息

NEBNext 接头采用了一种新型的发卡环状结构，能以更高的效率连接末端经修复和加 dA 尾的 DNA 片段。环状结构 DNA 中包含一个 U，当 U 被 USER® 酶（UDG 和 Endo VIII 的混合物）切掉后，环状结构打开，使它可以成为 PCR 反应的底物。通过 NEBNext Index 引物在 PCR 步骤中引入 Barcode，从而有助于实现多重测序文库制备。该试剂盒包含 8 种 i5 Index 引物和 12 种 i7 Index 引物。

NEBNext 接头引物可与 NEBNext 产品以及其它兼容 Illumina 平台的标准文库制备流程结合使用。

还提供双端 Index 引物（NEB #E7600）。还提供 12 种和 96 种单端 Index 引物（NEB #E7335、 NEB #E7500、NEB #E7710、NEB #E7730、NEB #E6609）。也提供甲基化的 NEBNext 接头（NEB #E7535），可用于重亚硫酸盐测序流程。

双端 Index 引物实验流程

产品类别:

NEBNext® Multiplex Oligos (Adaptors & Primers) Products

• 试剂盒组成

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  E7780S     -20       NEBNext Adaptor for Illumina E7601AVIAL -20 1 x 0.96 ml Not Applicable   USER® Enzyme E7602AVIAL -20 1 x 0.288 ml Not Applicable   NEBNext® i509 Primer E7781AVIAL -20 1 x 0.06 ml 10 µM   NEBNext® i510 Primer E7782AVIAL -20 1 x 0.06 ml 10 µM   NEBNext® i511 Primer E7783AVIAL -20 1 x 0.06 ml 10 µM   NEBNext® i512 Primer E7784AVIAL -20 1 x 0.06 ml 10 µM   NEBNext® i513 Primer E7785AVIAL -20 1 x 0.06 ml 10 µM   NEBNext® i514 Primer E7786AVIAL -20 1 x 0.06 ml 10 µM   NEBNext® i515 Primer E7787AVIAL -20 1 x 0.06 ml 10 µM   NEBNext® i516 Primer E7788AVIAL -20 1 x 0.06 ml 10 µM   NEBNext® i713 Primer E7789AVIAL -20 1 x 0.04 ml 10 µM   NEBNext® i714 Primer E7790AVIAL -20 1 x 0.04 ml 10 µM   NEBNext® i715 Primer E7791AVIAL -20 1 x 0.04 ml 10 µM   NEBNext® i716 Primer E7792AVIAL -20 1 x 0.04 ml 10 µM   NEBNext® i717 Primer E7793AVIAL -20 1 x 0.04 ml 10 µM   NEBNext® i718 Primer E7794AVIAL -20 1 x 0.04 ml 10 µM   NEBNext® i719 Primer E7795AVIAL -20 1 x 0.04 ml 10 µM   NEBNext® i720 Primer E7796AVIAL -20 1 x 0.04 ml 10 µM   NEBNext® i721 Primer E7797AVIAL -20 1 x 0.04 ml 10 µM   NEBNext® i722 Primer E7798AVIAL -20 1 x 0.04 ml 10 µM   NEBNext® i723 Primer E7799AVIAL -20 1 x 0.04 ml 10 µM   NEBNext® i724 Primer E7800AVIAL -20 1 x 0.04 ml 10 µM

• 相关产品

  相关产品

  • e7760-nebnext-ultra-ii-directional-rna-library-prep-kit-for-illumina
  • e7765-nebnext-ultra-ii-directional-rna-library-prep-with-sample-purification-beads
  • NEBNext^® Ultra^™ II RNA 非链特异性文库制备试剂盒
  • e7775-nebnext-ultra-ii-rna-library-prep-with-sample-purification-beads
  • NEBNext® Ultra™ II FS DNA 文库制备试剂盒 – 含片段化酶
  • NEBNext® Ultra™ II FS DNA 文库制备试剂盒 – 含片段化酶（含纯化磁珠）
  • NEBNext^® Ultra™ II DNA 文库制备试剂盒
  • NEBNext^® Ultra^™ II DNA 文库制备试剂盒（含纯化磁珠）
  • NEBNext® Ultra™ II 连接模块
  • NEBNext® Ultra™ DNA 文库制备试剂盒
  • NEBNext® Ultra 连接模块
  • NEBNext® 快速连接模块

操作说明、说明书 & 用法

• 操作说明

  1. Setting up the PCR Reaction – NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) (E7780)

• 说明书

  产品说明书包含产品使用的详细信息、产品配方和质控分析。

  • manualE7780

• 使用指南

  • E7780_DualIndex_IlluminaLRM
  • Forward Strand Workflow Sample Sheet for E7780: NovaSeq 6000 with v1.0 reagent kits, MiniSeq with Rapid reagent kits, MiSeq, HiSeq 2000/2500 (paired-end flow cell), HiSeq 3000/4000 (single-read flow cell)
  • Reverse Complement Workflow Sample Sheet for E7780: iSeq 100, MiniSeq with Standard reagent kits, NextSeq Systems, NovaSeq 6000 with v1.5 reagent kits, HiSeq 2000/2500 (single-read flow cell), HiSeq 3000/4000 (paired-end flow cell)

工具 & 资源

• 选择指南

  • NEBNext® Multiplex Oligos Selection Chart

FAQs & 问题解决指南

• FAQs

  1. Where can I find the sample sheets for these Multiplex Oligos?
  2. Are NEBNext^® Multiplex Oligos for Illumina^® (Dual Index Primers Set 2) supplied with library prep reagents?
  3. Can I use NEBNext^® Multiplex Oligos for Illumina^® (Dual Index Primers Set 2) for library preparation from DNA and RNA samples?
  4. How many libraries can I prepare with the NEBNext^® Multiplex Oligos for Illumina^® (Dual Index Primers Set 2)?
  5. For pooling <96 samples, what combination of indices should I use?
  6. Do I need to spike in custom sequencing primers when sequencing libraries are made with NEBNext^® Multiplex Oligos for Illumina^® (Dual Index Primers Set 2) on Illumina sequencing instruments?
  7. Can I perform single read runs and still get both index sequences? Can I run both single read and paired-end recipes with dual-indexed libraries?
  8. Is the USER^® Enzyme in the NEBNext Oligos kits identical in concentration to the standalone product?
  9. How do I use the NEB provided Sample Sheet for my specific instrument?
  10. Are dual indexed libraries compatible with single end sequencing?
  11. What sequences need to be trimmed for NEBNext libraries that are sequenced on an Illumina instrument?