SbfI | NEB酶试剂 New England Biolabs

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SbfI

我们很高兴宣布，我们即将完成把所有反应缓冲液转换为无 BSA（牛血清白蛋白）缓冲液。从 2021 年 4 月开始，NEB 将含有 BSA 的反应缓冲液转换为含有重组白蛋白（rAlbumin）的缓冲液，用于限制性内切酶和某些 DNA 修饰酶。网站上的所有信息均已根据此变化更新。如需了解更多信息，请访问 www.neb.cn/BSA-free。

  • Catalog # R0642 was discontinued on December 15, 2021
SbfI | NEB酶试剂 New England Biolabs
同裂酶 | 单字母标示 | Pronunciation: SbfI | NEB酶试剂 New England Biolabs
  • 符合省时酶（Time-Saver™）标准，可在 5-15 分钟内完成酶切
  • 在 rCutSmart™ 缓冲液中具有 100% 活性（超过 210 种内切酶兼容于此缓冲液），便于进行双酶切
  • 本酶可提供高保真（HF®）版（NEB #R3642），随附 rCutSmart 缓冲液
  • 限制性内切酶酶切位点：CCTGCA/GG

精选视频

SbfI | NEB酶试剂 New England Biolabs

Reduce Star Activity with High-Fidelity Restriction Enzymes

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产品信息

本酶提供高保真版 SbfI-HF®（NEB #R3642）

高保真（HF）限制性内切酶在 rCutSmart 缓冲液中具有 100% 活性；统一缓冲液意味着更加直接、简化的样品处理过程。HF 内切酶还会显著降低星号活性。所有 HF 内切酶均符合省时酶（Time-Saver）标准，可在 5-15 分钟内酶切底物 DNA，也可实现过夜酶切，而且不会造成 DNA 降解。HF 限制性内切酶在改造时便将性能作为重要指标，可在更宽的条件范围下具有完全活性，最大限度地减少非特异性酶切产物，同时为实验设计提供灵活性。

产品来源

大肠杆菌菌株，携带有来自链霉菌属（Streptomyces）Bf-61（S.K. Degtyarev）的 SbfI 基因。

产品类别:
Discontinued Products

  • 特性和用法

    单位定义

    一个单位是指在 50 µl 的总反应体系中，37℃ 下，1 小时内酶切 1 µg λ DNA 所需的酶量。

    反应条件

    1X rCutSmart™ 缓冲液
    Incubate at 37°C

    1X rCutSmart™ 缓冲液
    50 mM Potassium Acetate
    20 mM Tris-acetate
    10 mM Magnesium Acetate
    100 µg/ml Recombinant Albumin
    (pH 7.9 @ 25°C)

    在不同缓冲液中的活性

    NEBuffer™ r1.1: 50%
    NEBuffer™ r2.1: 25%
    NEBuffer™ r3.1: <10%
    rCutSmart™ Buffer: 100%

    稀释兼容性

    • 稀释液 A

    贮存溶液

    10 mM Tris-HCl
    50 mM NaCl
    1 mM DTT
    0.1 mM EDTA
    200 µg/ml BSA
    50% Glycerol
    pH 7.4 @ 25°C

    热失活

    80°C for 20 min

    甲基化敏感性

    dam 甲基化: 不敏感
    dcm 甲基化: 不敏感
    CpG甲基化: 不敏感

    同裂酶

    SbfI-HF
    SdaI
    Sse8387I

  • 相关产品

    相关产品

    • SbfI-HF®
    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

    单独销售的组分

    • rCutSmart™ 缓冲液

  • 注意事项
    1. SbfI 是 Sse8387I 的同裂酶。
    2. 对 CpG、dcm 或 dam 甲基化不敏感。
    3. 甘油浓度 ＞5% 条件下可能出现星号活性

操作说明、说明书 & 用法

  • 操作说明
    1. Optimizing Restriction Endonuclease Reactions
    2. Restriction Digest Protocol
    3. Double Digest Protocol with Standard Restriction Enzymes

  • 使用指南
    • Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
    • Activity of Restriction Enzymes in PCR Buffers
    • Cleavage Close to the End of DNA Fragments
    • Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
    • Double Digests
    • Heat Inactivation
    • Restriction Endonucleases – Survival in a Reaction
    • Restriction Enzyme Diluent Buffer Compatibility
    • Restriction Enzyme Tips
    • Single Letter Codes
    • Star Activity

工具 & 资源

  • 选择指南
    • Alphabetized List of Recognition Sequences
    • Compatible Cohesive Ends and Generation of New Restriction Sites
    • Dam-Dcm and CpG Methylation
    • Frequencies of Restriction Sites
    • Isoelectric Points (pI) for Restriction Enzymes
    • Isoschizomers
    • NEB Diluent and Buffer Table
    • Time-Saver™ Qualified Enzymes

  • Web 工具
    • DNA Sequences and Maps Tool
    • Double Digest Finder
    • Enzyme Finder
    • NEBcutter™ v3.0
    • NEBioCalculator®
    • REBASE®

FAQs & 问题解决指南

  • FAQs
    1. Is SbfI a Time-Saver™ Qualified enzyme?
    2. Is SbfI activity sensitive to dam, dcm or mammalian CpG methylation?
    3. How many base pairs should be added at the end of a PCR primer next to the SbfI recognition site to guarantee that SbfI will cut properly?
    4. Does SbfI have any neoschizomers?
    5. Is there a High Fidelity version of SbfI?
    6. Does SbfI exhibit star activity?
    7. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
    8. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
    9. Why is my Restriction Enzyme not cutting DNA?
    10. Why do I see additional DNA bands on my gel after a restriction digest?
    11. Why do I see a DNA smear on an agarose gel after a restriction digest?
    12. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
    13. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    SbfI | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

    更多引用文献

    • Qiu S, Bergero R, Guirao-Rico S, Campos JL, Cezard T, Gharbi K, Charlesworth D. (2016) RAD mapping reveals an evolving, polymorphic and fuzzy boundary of a plant pseudoautosomal region. Mol Ecol; Jan;25(1), 414-30.
    • Qiu S, Bergero R, Guirao-Rico S, Campos JL, Cezard T, Gharbi K, Charlesworth D. (2016) RAD mapping reveals an evolving polymorphic and fuzzy boundary of a plant pseudoautosomal region Mol Ecol; Jan;25(1), 414-30.
    • Díaz-Arce N, Arrizabalaga H, Murua H, Irigoien X, Rodríguez-Ezpeleta N. (2016) RAD-seq derived genome-wide nuclear markers resolve the phylogeny of tunas. Mol Phylogenet Evol; Sep;102, 202-7.
    • Araneda C, Larraín MA, Hecht B, Narum S. (2016) Adaptive genetic variation distinguishes Chilean blue mussels (Mytilus chilensis) from different marine environments. Ecol Evol; Apr 26,
    • Rutledge LY, Devillard S, Boone JQ, Hohenlohe PA, White BN. (2015) RAD sequencing and genomic simulations resolve hybrid origins within North American Canis. Bio Lett; Jul;11(7)., pii: 20150303. .
    • Liu S, Vallejo RL, Palti Y, Gao G, Marancik DP, Hernandez AG, Wiens GD. (2015) Identification of single nucleotide polymorphism markers associated with bacterial cold water disease resistance and spleen size in rainbow trout. Front Genet; Sep 24;6:298.
    • Mathers TC, Hammond RL, Jenner RA, Hänfling B, Atkins J, Gómez A. (2015) Transition in sexual system and sex chromosome evolution in the tadpole shrimp Triops cancriformis. Heredity (Edinb); Jul;115(1), 37-46.
    • Zhou Z, Liu S, Dong Y, Gao S, Chen Z, Jiang J, Yang A, Sun H, Guan X, Jiang B, Wang B. (2015) High-Density Genetic Mapping with Interspecific Hybrids of Two Sea Urchins, Strongylocentrotus nudus and S. intermedius, by RAD Sequencing. PLoS One; Sep 23;10(9),
    • Longo G, Bernardi G. (2015) The evolutionary history of the embiotocid surfperch radiation based on genome-wide RAD sequence data. Mol Phylogenet Evol; Jul;88, 55-63.

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • R0642S_L_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • R0642S_L_v1_0471211
    • R0642S_L_v1_0471302
    • R0642S_L_v1_0471303
    • R0642S_L_v1_0471309
    • R0642S_L_v1_0471402
    • R0642S_L_v1_0471408
    • R0642S_L_v1_0471501
    • R0642S_L_v1_0471507
    • R0642S_L_v1_0471512
    • R0642S_L_v1_0471608
    • R0642S_L_v1_0471701
    • R0642S_L_v1_0471708
    • R0642S_L_v1_0471804
    • R0642L_v1_10008626
    • R0642L_v1_10025850
    • R0642S_v1_10025852
    • R0642S_v1_10041079
    • R0642L_v1_10046527
    • R0642L_v1_10056080
    • R0642S_v1_10057661
    • R0642S_v1_10078413
    • R0642S_v1_10091207
    • R0642L_v1_10084456
    • R0642S_v1_10093315
    • R0642L_v1_10100850
    • R0642S_v1_10117588
    • R0642L_v1_10114608

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • SbfI