- 产品特性
- 相关资料
- Q&A
- 参考文献
GFP-CERTIFIED® 细胞凋亡/坏死检测试剂盒
GFP-CERTIFIED® Apoptosis/Necrosis detection kit
可进行区分正常细胞、早期凋亡细胞、晚期凋亡细胞和坏死细胞的多重检测，可与 GFP 和其他绿色荧光探针兼容。
● 可以兼容 GFP 和其他绿色荧光探针
● 容易区分正常细胞、早期凋亡细胞、晚期凋亡细胞和坏死细胞
● 已根据荧光显微镜和流式细胞仪等应用进行过优化
● 适用于死亡途径分析及药物、毒素研究
从凋亡到坏死的过渡是一个松散定义的连续体。质膜细胞外表面上磷脂酰丝氨酸（PS）的暴露仍是早期细胞凋亡的共同标志。这时候在Ca2+存在下，磷脂结合蛋白例如 Annexin V 与 PS 具有亲和力。由于 Annexin V 不具有细胞渗透性，所以这种胞外PS的结合是早期凋亡细胞对具有选择性的。
同样，质膜完整性的丧失，如不具有膜渗透性DNA嵌入染料若能标记出细胞核，则是表明晚期细胞凋亡和坏死的直接方法。考虑到Annexin V通常与荧光素（又称FITC）偶联，检测绿色荧光蛋白（GFP）表达细胞中的凋亡时可能会出现问题。以上两个荧光团的发射图谱会发生光谱重叠，使信号之间的区分变得困难或不可能，从而损害了总体上的数据质量。GFP-CERTIFIED® 细胞凋亡/坏死检测系统专为表达GFP的细胞系以及表达蓝色或青色荧光蛋白（BFP、CFPs）的细胞而设计，同时也可以应用于不带荧光蛋白的细胞的凋亡和坏死的细胞。另外，本试剂盒适合用于活细胞或结合了探针（如标记抗体、或与荧光素或香豆素显示相似光谱特性的其他荧光偶联物）的细胞。本试剂盒包含了用于确定细胞凋亡及坏死的早期和晚期阶段中所需的全部试剂。Annexin V -EnzoGold（增强型Cyanine-3）偶联物能够检测不同于荧光素或GFP的细胞凋亡。坏死检测试剂（红色）类似于发出红色的染料7-AAD，可促进晚期细胞凋亡和坏死检测。本试剂盒还包含了可用作阳性对照的凋亡诱导剂（Staurosporine）。检测试剂可单独使用或分别与其他单独或多重应用组合使用。本试剂盒还可以通过流式细胞仪和荧光/共焦显微镜提供快速、准确的检查结果。
GFP、Annexin V-Cyanine 3 偶联物和坏死检测试剂（红色）的激发（阴影线）和发光（固体）光谱。
三种染料都可以轻易地被 488 nm 激光源激发。所有荧光团之间的发射峰值容易区分。
GFP-CERTIFIED® 细胞凋亡/坏死检测试剂盒（ENZ-51002）
流式细胞仪。用0.2％DMSO（panel A）模拟诱导 Jurkat 细胞，或用 2 μM 星形孢菌素（panel B）在37°C下诱导 Jurkat 细胞４h。经过处理后，在含有 Annexin V-Cyanine 3 和坏死检测试剂（红色；一种远红色发光的 DNA 嵌入染料）的缓冲液中孵育细胞，然后用 488 nm 激光的流式细胞仪进行分析，用 FL2（细胞凋亡）和 FL3（坏死）通道进行荧光检测。
模拟诱导的细胞凋亡和坏死主要呈阴性。处理４h 后，会出现３种细胞群：
（1）可以存活且不凋亡、不坏死的细胞（Annexin V-Cyanine 3、坏死检测试剂呈阴性）；
（2）正在经历凋亡的细胞（Annexin V-Cyanine 3 呈阳性和 NDR 呈阴性）；
（3）处于晚期凋亡和早期坏死的细胞（Annexin V-Cyanine 3 和坏死检测试剂呈阳性）。
用 2.5 µM 星形孢菌素刺激 Jurkat 细胞 0-6 h。使用 Jurkat 悬浮细胞的流式细胞仪结果表现为早期凋亡（Annexin V-EnzoGold）和晚期凋亡/坏死（Annexin V-EnzoGold 和坏死染色）。
◆产品规格
|
应用： |
流式细胞仪、荧光显微镜、荧光检测。 |
|
使用事项/稳定性： |
Annexin V-EnzoGold 和结合缓冲液应在４°C储存。 |
|
储存条件： |
避光。 |
|
运输： |
蓝冰运输。 |
|
短期储存温度： |
４°C |
|
试剂盒构成： |
凋亡检测试剂 (Annexin V-EnzoGold) |
|
技术信息/产品说明： |
应用说明： Neurotoxicants |
|
监管状况： |
RUO-仅供研究用 |
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Phlorofucofuroeckol Improves Glutamate-Induced Neurotoxicity through Modulation of Oxidative Stress-Mediated Mitochondrial Dysfunction in PC12 Cells: J.J. Kim, et al.; PLoS One 11, e0163433 (2016), 摘要; 全文 |
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PKLR promotes colorectal cancer liver colonization through induction of glutathione synthesis: A. Nguyen, et al.; J. Clin. Invest. 126, 681 (2016), Application(s): Assessed Apoptosis, Flow cytometry, 摘要; 全文 |
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Growth-promoting and tumourigenic activity of c-Myc is suppressed by Hhex: V. Marfil, et al.; Oncogene 34, 3011 (2015), Application(s): Flow cytometry using HO15.19 and TGR-1 cells, 摘要; |
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Impaired oxidative phosphorylation regulates necroptosis in human lung epithelial cells: M.J. Koo, et al.; Biochem. Biophys. Res. Commun. 464, 875 (2015), Application(s): Apoptosis/necrosis assay, 摘要; |
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30. |
Influenza virus M2 targets cystic fibrosis transmembrane conductance regulator for lysosomal degradation during viral infection: J.D. Londino, et al.; FASEB J. 29, 2712 (2015), Application(s): Flow cytometry using HEK293 cells, 摘要; 全文 |
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Inhibition of autophagy exerts anti-colon cancer effects via apoptosis induced by p53 activation and ER stress: K. Sakitani, et al.; BMC Cancer 15, 795 (2015), Application(s): Flow cytometric analysis of apoptosis, 摘要; 全文 |
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32. |
N-terminal functional domain of Gasdermin A3 regulates mitochondrial homeostasis via mitochondrial targeting: P.H. Lin, et al.; J. Biomed. Sci. 22, 44 (2015), Application(s): Fluorescence microscopy using HaCaT and HEK293 cells, 摘要; 全文
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Novel carbocyclic curcumin analog CUR3d modulates genes involved in multiple apoptosis pathways in human hepatocellular carcinoma cells.: K.S. Bhullar, et al. ; Chem. Biol. Interact. 242, 107 (2015), Application(s): Fluorescence microscopy, 摘要; |
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NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival: J.M. Calandria, et al.; Cell Death Differ. 22, 1363 (2015), Application(s): Assay, 摘要; |
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ROS-induced oxidative stress and apoptosis-like event directly affect the cell viability of cryopreserved embryogenic callus in Agapanthus praecox: D. Zhang, et al.; Plant Cell Rep. 34, 1499 (2015), 摘要;
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The Adhesion GPCR CD97/ADGRE5 inhibits apoptosis: C.C. Hsiao, et al.; Int. J. Biochem. Cell. Biol. 65, 197 (2015), Application(s): Flow cytometry using HT1080 cells, 摘要; |
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37. |
The effect of plasma-derived activated protein C on leukocyte cell-death and vascular endothelial damage: T. Iba, et al.; Thromb. Res 135, 963 (2015), Application(s): Fluorescence Microscopy using leukocytes, 摘要; |
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38. |
Accumulation of cytosolic calcium induces necroptotic cell death in human neuroblastoma: M. Nomura, et al.; Cancer Res. 74, 1056 (2014), 摘要; |
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39. |
Apoptotic and inhibitory effects on cell proliferation of hepatocellular carcinoma HepG2 cells by methanol leaf extract of Costus speciosus: S.V. Nair, et al.; Biomed. Res. Int. 2014, Article ID 637098 (2014), Application(s): Measurement by flow cytometry, 摘要; 全文 |
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40. |
Combination of antithrombin and recombinant thrombomodulin modulates neutrophil cell-death and decreases circulating DAMPs levels in endotoxemic rats: T. Iba, et al.; Throm. Res. 134, 169 (2014), Application(s): Detection Kit using live cells, 摘要; |
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41. |
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| 产品编号 | 产品名称 | 产品规格 | 产品等级 |
| ENZ-51002-25 | GFP-CERTIFIED® Apoptosis/Necrosis detection kit 细胞凋亡/坏死检测试剂盒（荧光显微镜&流式）（GFP细胞系） |
25 assays | |
| ENZ-51002-100 | GFP-CERTIFIED® Apoptosis/Necrosis detection kit 细胞凋亡/坏死检测试剂盒（荧光显微镜&流式）（GFP细胞系） |
100 assays |