ERK1/2, mAb (12D4)

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    • 货号：

      ALX-804-061-C100

    • 保存条件：

      ‘-20℃

    • 供应商：

      上海金畔生物科技有限公司

    • 规格：

      100 µg

    免疫动物：小鼠免疫原：Peptide comprising the pThr-Glu-pTyr motif in activated ERK1 and ERK2 conjugated to KLH.应用：ELISAImmunocytochemistry (1-10µg/ml)ImmunohistochemistryImmunoprecipitation (1-10µg per 106 pervanadate-treated A431 or HepG2 cells)Western Blot (0.5µg/ml for HRPO/ECL detection).Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.特异性：Recognizes the pThr-Glu-pTyr motif of activated human, mouse, rat and dog ERK1 and ERK2. Requires phosphorylation both at the threonine and the tyrosine site and does not cross-react with the non-phosphorylated form of the protein. Does not cross-react with activated JNK or p38.

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