免疫动物：兔免疫原：Synthetic peptide corresponding to aa 20-34 of human Bcl-2.应用：Immunohistochemistry:冷冻切片：5µg/ml；不需预处理。石蜡切片：2.5µg/ml；推荐用压力锅进行抗原修复。For both: DAB detection. Intestine or human lymph node can be used as positive control, brain tissue as negative control (only random cells will stain for Bcl-2 in brain tissue).Western Blot: 5µg/ml; ECL detection. MCF-7 cells can be used as a positive control, normal rabbit IgG as negative control.Titration is recommended for optimal results in individual systems.特异性：Recognizes human, mouse and opossum Bcl-2. This antibody is particularly useful for the determination of Bcl-2 expression levels in species other than human.。