for Alzheimer’s Disease (AD)

• BF-168 (Wako Cat. #029-16361): Senile plaque-selective fluorescent probe
  BF-168 is a probe for senile plaques (SPs). It selectively binds SPs in AD brain sections and recognizes Aβ 1-42 positive diffuse plaques.
• BF-170 (Wako Cat. #026-16371): Neurofibrillary tangle change-selective fluorescent probe
  BF-170 is a probe for neurofibrillary tangles (NFTs). It exhibits greater binding affinity to Tau fibrils such as NFTs, neuropil threads and paired helical filament than to Aβ fibrils.

B-168

Solubility: Soluble in methanol and ethanol.
λex: 380 ~ 420 (nm)
λem: > 450 (nm)

Procedure:

1. Deparaffinize the section on 1 slide glass plate.
2. Immerse the section with 10% formalin neutral buffer for 60 minutes.
3. Wash the section with PBS for 5 minutes.
4. Blanch the section with 0.25% potassium permanganate solution for 20 minutes.
5. Wash the section twice with PBS for 2 minutes.
6. Immerse the section with 0.1% potassium metabisulfite and 0.15% oxalic acid for seconds.
7. Wash the section three times with PBS for 2 minutes.
8. Immerse the quenched tissue section with 100 μmol/L BF-168 solution containing 50% ethanol for 10 minutes.
9. Dip the section 5 times with water for a few seconds.
10. Observe the section with fluorescence microscope.

References:

1. Kitamoto, T., Ogomori, K., Tateishi, J., and Prusiner, S. B.: Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids., Lab. Invest., 57, 230-236 (1987)
2. Okamura, N., et al.: Styrylbenzoxazole derivatives for in vivo imaging of amyloid plaques in the brain, J. Neurosci., 24, 2535-2541 (2004)
3. Okamura, N., Kudo, Y., et al.: Quinoline and benzimidazole derivatives: candidate probes for in vivo imaging of tau pathology in Alzheimer’s disease, J. Neurosci., 25, 10857-10862 (2005)