Novel acidic organelle-selective dye, suitable for live cell imaging of lysosomes Long wavelength red emission that is easily multiplexed with common fluorescent dyes (coumarin, FITC, Cyanine 3) and fluorescent proteins (BFP, CFP, GFP, YFP) No photo conversion to a green emitting dye and no complicating metachromatic dual emission artifacts, facilitating analysis with GFP, FITC and other fluorescent probes Highly resistant to photobleaching and concentration quenching, ensuring strong, consistent fluorescence signal, even after extended viewing periods GFP-Certified™ Lyso-ID® Red Lysosomal Detection Kit is an ideal choice for fluorescence co-localization imaging with GFP-tagged proteins, providing spectrally pure red signal. By contrast, many red-emitting lysosomal probes photoconvert to green fluorescent species or display metachromatic artifacts wherein emission occurs both in the red and green regions of the spectrum, leading to spurious results in GFP co-localization experiments [Freundt et al (2007) Cell Res. 17(11): 956- 958; Nadrigny et al (2007) Biophys J. 93(3):969-980]. The GFP-Certified™ Lyso-ID® Red Lysosomal Detection Kit contains a proprietary acidic organelle-selective dye suitable for staining live cells. The dye is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms. This kit is specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs). The Lyso-ID® Red dye has been validated for utility in live cell imaging applications, showing an appropriate response to treatment with well characterized lysosomal perturbation agents. A lysosome perturbation agent, chloroquine, is provided as a positive control for monitoring changes in lysosome number and volume. A nuclear counterstain (Hoechst 33342) is provided to highlight this organelle as well. 联系方式： 上海金畔生物科技有限公司－北京 地址：北京市朝阳区建国路88号SOHO现代城4号楼11楼1106室 邮编：100022 电话：+86 10 8580 4838传真：+86 10 8580 4733 网址：www.boppard.cn E-mail：info@boppard.cn业务QQ：1433642494 上海金畔生物科技有限公司－上海 地址：上海市长寿路1118号悦达国际大厦B幢10层J室 邮编：200042 电话：+86 21 6288 4751 6212 8663 传真：+86 21 6288 4752 网址：www.boppard.cn E-mail：info@boppard.cn业务QQ：562608361 上海金畔生物科技有限公司－广州地址：广州市先烈中路69号东山广场30楼3003室 邮编：510095 电话：+86 20 8732 6381 2237 1022 传真：+86 20 8732 6382 网址：www.boppard.cn E-mail：info@boppard.cn 业务QQ：1043735153 上海金畔生物科技有限公司－香港地址：香港新界荃湾沙咀道11-19号达贸中心11楼1111室 电话：+852 2799 9019 传真：+852 2799 9808 E-mail：info@boppard.com.hk 上海金畔生物科技有限公司－陕西 电话：15094076206网址：www.boppard.cn E-mail：info@boppard.cn