Alkaline Phosphatase (ALP) is distributed in a variety of tissues such as liver, bone, and small intestine in animals. The change of the enzyme activity in tissues is an im-portant hallmark for physiological phenomena as osteo-genesis and so on. This kit is for Alkaline Phosphatase assay in a simultaneous multi-sample assay format with a microplate using p-Nitrophenylphosphate as a substrate.

[Kit Contents]

1. Substrate Tablet 20 tablets
   (p-Nitrophenylphosphate Disodium 6.7 mmol/L, after dissolving)
2. Buffer Solution 100 mL
   (2.0 mmol/L MgCl₂, 0.1mol/L Carbonate Buffer, pH 9.8)
3. Stop Solution 100 mL(0.2 mol/L Sodium Hydroxide Solution)
4. Standard Solution 10 mL
   (0.5 mmol/L p-Nitrophenol Solution)

[Assay principle]
p-Nitrophenylphosphate is hydrolyzed into p-Nitrophenol and Phosphoric Acid in the carbonate buffer (pH 9.8) in the presence of Alkaline Phosphatase in sample. Released p-Nitrophenol showing yellow color is optically measured at 405 nm wavelength as the enzyme activity.

[Features]
Dynamic assay range: > 0.06 mmol/L
Standard assay range: 0 ~ 0.5 mmol/L (The activity at concentration more than 0.5 mmol/L can be determined by extrapolation due to its linearity.)
Reproducibility: C.V. < 10%

[Reference]
Yamamoto, M., Takahashi, Y., Tabata, Y.: Biomaterials, 24 (24), 4375 (2003).