组成部分

状态

容量

(A)   抗体固相化 96 孔板

洗净后使用

96 wells（8×12）／1块

(B)   标准溶液（2,000 ng/mL）

稀释后使用

200 μL／1 瓶

(C)   缓冲液

即用

60 mL／1 瓶

(D)   HRP标识抗脂联素抗体

稀释后使用

100 μL／1 瓶

(F)   显色液（TMB）

即用

12 mL／1 瓶

(H)   反应终止液（1M H₂SO₄）※小心轻放

即用

12 mL／1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL／1 瓶

封板膜

3 张

使用说明书

1 份

动物种类

对象物质

反应性和交叉率(%)

Mouse

Adiponectin(HMW)

100

Adiponectin(Hexamer)

<5

Adiponectin(Trimer)

不存在交叉反应

Adiponectin(Monomer)

不存在交叉反应

MCH

不存在交叉反应

TNF-α

不存在交叉反应

IFN-γ

不存在交叉反应

Insulin

不存在交叉反应

Leptin

不存在交叉反应

Rat

Adiponectin(HMW)

100

Adiponectin(Monomer)

不存在交叉反应

TNF-α

不存在交叉反应

IFN-γ

不存在交叉反应

Insulin

不存在交叉反应

Leptin

不存在交叉反应

样品

A

B

1

29.5

129

2

30.7

125

3

29.8

128

4

29.0

126

5

29.6

126

mean

29.7

127

SD

0.631

1.89

CV(%)

2.12

1.49

测量日/样品

C

D

E

第0天

196

126

62.5

第1天

192

130

59.1

第2天

196

125

60.7

第3天

190

125

60.3

mean

193

127

60.7

SD

2.63

2.27

1.41

CV(%)

1.36

1.79

2.33

添加量

实测值

回收量

回收率(%)

0

68.5

–

–

35.0

103

34.5

98.6

65.0

132

63.5

97.7

95.0

165

96.9

102

添加量

实测值

回收量

回收率(%)

0

23.3

–

–

18.0

40.3

17.0

94.4

26.0

50.6

27.3

105

32.0

55.5

32.2

101

ELISA试剂盒选择指南①②

1.

Maternal intake of grape seed procyanidins during lactation induces insulin resistance and an adiponectin resistance-like phenotype in rat offspring. Caimari A, Marine-Casado R, Boque N, Crescenti A, Arola L, Del Bas JM. Sci Rep. 2017 Oct 3;7(1):12573.

2.

Effects of β-Glucan Content and Pearling of Barley in Diet-Induced Obese Mice. Seiichiro Aoe, Yasunori Ichinose, Noriko Kohyama, Kozo Komae, Asuka Takahashi, Toji Yoshioka, and Takashi Yanagisawa. Posted online on 27 Sep 2017.

https://doi.org/10.1094/CCHEM-04-17-0083-R

3.

The cannabinoid ligand LH-21 reduces anxiety and improves glucose handling in diet-induced obese pre-diabetic mice. Romero-Zerbo SY, Ruz-Maldonado I, Espinosa-Jimenez V, Rafacho A, Gomez-Conde AI, Sanchez-Salido L, Cobo-Vuilleumier N, Gauthier BR, Tinahones FJ, Persaud SJ, Bermudez-Silva FJ. Sci Rep. 2017 Jun 21;7(1):3946.

4.

Moringa oleifera from Cambodia Ameliorates Oxidative Stress, Hyperglycemia, and Kidney Dysfunction in Type 2 Diabetic Mice. Tang Y, Choi EJ, Han WC, Oh M, Kim J, Hwang JY, Park PJ, Moon SH, Kim YS, Kim EK. J Med Food. 2017 May;20(5):502-510

5.

Gelidium amansii extract ameliorates obesity by down-regulating adipogenic transcription factors in diet-induced obese mice. Kang JH, Lee HA, Kim HJ, Han JS. Nutr Res Pract. 2017 Feb;11(1):17-24.

6.

Oleuropein aglycone enhances UCP1 expression in brown adipose tissue in high-fat-diet-induced obese rats by activating β-adrenergic signaling. Oi-Kano Y, Iwasaki Y, Nakamura T, Watanabe T, Goto T, Kawada T, Watanabe K, Iwai K. J Nutr Biochem. 2017 Feb;40:209-218.

7.

Mild Hyperbaric Oxygen Inhibits Growth-related Decrease in Muscle Oxidative Capacity of Rats with Metabolic Syndrome. Takemura A, Ishihara A. J Atheroscler Thromb. 2017 Jan 1;24(1):26-38.

8.

Germinated Pigmented Rice (Oryza Sativa L. cv. Superhongmi) Improves Glucose and Bone Metabolisms in Ovariectomized Rats. Chung SI, Ryu SN, Kang MY. Nutrients. 2016 Oct 21;8(10).

9.

Sodium alginate prevents progression of non-alcoholic steatohepatitis and liver carcinogenesis in obese and diabetic mice. Miyazaki T, Shirakami Y, Kubota M, Ideta T, Kochi T, Sakai H, Tanaka T, Moriwaki H, Shimizu M. Oncotarget. 2016 Mar 1;7(9):10448-58.

10.

Biotin augments acetyl CoA carboxylase 2 gene expression in the hypothalamus, leading to the suppression of food intake in mice. Sone H, Kamiyama S, Higuchi M, Fujino K, Kubo S, Miyazawa M, Shirato S, Hiroi Y, Shiozawa K. Biochem Biophys Res Commun. 2016 Jul 29;476(3):134-9.

11.

Reduced rat plasma lysophosphatidylglycerol or lysophosphatidic acid level as a biomarker of aristolochic acid-induced renal and adipose dysfunctions. Tsutsumi T, Okamoto Y, Yamakawa S, Bingjun C, Ishihara A, Tanaka T, Tokumura A. Life Sci. 2016 Jul 15;157:208-16.

12.

Fermentation of purple Jerusalem artichoke extract to improve the α-glucosidase inhibitory effect in vitro and ameliorate blood glucose in db/db mice. Wang Z, Hwang SH, Lee SY, Lim SS. Nutr Res Pract. 2016 Jun;10(3):282-7.

13.

Anti-diabetic effects of luteolin and luteolin-7-O-glucoside on KK-Ay mice. Zang Y, Igarashi K, Li Y. Biosci Biotechnol Biochem. 2016 May 12:1-7.

14.

The combination of maternal and offspring high-fat diets causes marked oxidative stress and development of metabolic syndrome in mouse offspring. Ito J, Nakagawa K, Kato S, Miyazawa T, Kimura F, Miyazawa T. Life Sci. 2016 Apr 15;151:70-5.

15.

Wasabi leaf extracts attenuate adipocyte hypertrophy through PPARγ and AMPK. Oowatari Y, Ogawa T, Katsube T, Iinuma K, Yoshitomi H, Gao M. Biosci Biotechnol Biochem. 2016 May 3:1-8.

16.

Biotin augments acetyl CoA carboxylase 2 gene expression in the hypothalamus, leading to the suppression of food intake in mice. Sone H, Kamiyama S, Higuchi M, Fujino K, Kubo S, Miyazawa M, Shirato S, Hiroi Y, Shiozawa K. Biochem Biophys Res Commun. 2016 May 12

17.

Oral administration of Aloe vera gel powder prevents UVB-induced decrease in skin elasticity via suppression of overexpression of MMPs in hairless mice. Saito M, Tanaka M, Misawa E, Yao R, Nabeshima K, Yamauchi K, Abe F, Yamamoto Y, Furukawa F. Biosci Biotechnol Biochem. 2016 Apr 4:1-9.

18.

The combination of maternal and offspring high-fat diets causes marked oxidative stress and development of metabolic syndrome in mouse offspring. Ito J, Nakagawa K, Kato S, Miyazawa T, Kimura F, Miyazawa T. Life Sci. 2016 Apr 15;151:70-75.

19.

Total and high molecular weight adiponectin levels in the rat model of post-myocardial infarction heart failure. Kalisz M, Baranowska B, Wolinska-Witort E, Maczewski M, Mackiewicz U, Tulacz D, Gora M, Martynska L, Bik W. J Physiol Pharmacol. 2015 Oct;66(5):673-80.

20.

Anti-obese and anti-diabetic effects of a mixture of daidzin and glycitin on C57BL/6J mice fed with a high-fat diet. Zang Y, Igarashi K, Yu C. Biosci Biotechnol Biochem. Vol.79(1), p117-23, 2015.

21.

Dietary Administration of Aloe Vera Gel Extract Inhibits Intestinal Polyp Formation in Min Mice Fed a High-Fat Diet. Chihara T, Shimpo K, Beppu H, Kaneko T, Higashiguchi T, Sonoda S, Tanaka M, Yamada M and Abe. Pharm Anal Acta 2015, 6:3

22.

Preventive effects of astaxanthin on diethylnitrosamine-induced liver tumorigenesis in C57/BL/KsJ-db/db obese mice. Ohno T, Shimizu M, Shirakami Y, Miyazaki T, Ideta T, Kochi T, Kubota M, Sakai H, Tanaka T, Moriwaki H. Hepatol Res. Jul 2015.

23.

Effects of liquid konjac on parameters related to obesity in diet-induced obese mice. Aoe S, Kudo H, Sakurai S. Biosci Biotechnol Biochem. Vol.79(7), p1141-6, Jul 2015.

24.

The anti-obesity and anti-diabetic effects of kaempferol glycosides from unripe soybean leaves in high-fat-diet mice. Zang Y, Zhang L, Igarashi K, Yu C. Food Funct. Vol.11;6(3), p834-41, Mar 2015.

25.

Ashitaba (Angelica keiskei) extract prevents adiposity in high-fat diet-fed C57BL/6 mice. Zhang T, Yamashita Y, Yasuda M, Yamamoto N, Ashida H. Food Funct. Vol.6(1), p134-144, Jan 2015.

26.

Anti-obese and anti-diabetic effects of a mixture of daidzin and glycitin on C57BL/6J mice fed with a high-fat diet. Zang Y, Igarashi K, Yu C. Bioscience, Biotechnology, and Biochemistry, Vol.79(1), 2015.

27.

Oolong, black and pu-erh tea suppresses adiposity in mice via activation of AMP-activated protein kinase. Yamashita Y, Wang L, Wang L, Tanaka Y, Zhang T, Ashida H. Food Funct. Vol.5(10), p2420-29, Oct 2014.

28.

Functional ingredients and cardiovascular protective effect of pumpkin seed oils. Al-Okbi SY., Mohamed DA., Kandi E., Ahmed EK., Mohammed SE. Source: Grasas y Aceites, Vol.65(1), p1-10, Jan-Mar 2014.

29.

The novel dipeptidyl peptidase-4 inhibitor teneligliptin prevents high-fat diet-induced obesity accompanied with increased energy expenditure in mice. Fukuda-Tsuru S., Kakimoto T., Utsumi H., Kiuchi S., Ishi S. European Journal of Pharmacology, Vol.723(15), p207-215, Jan 2014.

30.

Non-alcoholic steatohepatitis and preneoplastic lesions develop in the liver of obese and hypertensive rats: Suppressing effects of EGCG on the development of liver lesions. Kochi T., Shimizu M, Terakura D., Baba A., Ohno T., Kubota M., Shirakami Y., Tsurumi H., Tanaka T., Moriwaki H. Cancer Letters, Vol342(1), p60-69, Jan 2014.

31.

Anti-glycation Activity of Japanese Chestnut (Castanea crenata) Inner Skin Extract is Beneficial for Type 2 Diabetes in a Rat Model. Mizutani T., Shizuka F., Matsuzawa T., Amano Y., Arikawa Y. Anti-Aging Medicine, Vol.10(6), p112-119, 2014.

32.

Chronic Administration of Bovine Milk-Derived α-Lactalbumin Improves Glucose Tolerance via Enhancement of Adiponectin in Goto-Kakizaki Rats with Type 2 Diabetes. Yamaguchi M., Takai S. Biological and Pharmaceutical Bulletin, Vol.37(2014) No.3, p.404-408, 2014.

33.

Effects of Sleeve Gastrectomy on Lipid Metabolism in an Obese Diabetic Rat Model. Kawano, Y., Ohta, M., Hirashita, T., Masuda, T., Inomata, M., Kitano. S. Obesity Surgery, Vol.23(12), p1947-1956, Dec 2013.

34.

Quercetin glucosides promote ischemia-induced angiogenesis, but do not promote tumor growth. Sumi M., Tateishi N., Shibata H., Ohki T., Sat M. Life Sciences, Vol.93(22), p814-819, Nov 2013.

35.

I ntake of mulberry 1-deoxynojirimycin prevents diet-induced obesity through increases in adiponectin in mice. Tsuduki T., Kikuchi I., Kimura T., Nakagawa K., Miyazawa T. Food Chemistry, Vol.139(1-4), p16-23, Aug 2013.

36.

Effects of Sleeve Gastrectomy on Lipid Metabolism in an Obese Diabetic Rat Model. Y.Kawano, M.Ohta, T.Hirashita, T.Masuda, M.Inomata, S.Kitano. Obesity Surgery, Jul 2013.

37.

Rosehip Extract Inhibits Lipid Accumulation in White Adipose Tissue by Suppressing the Expression of Peroxisome Proliferator-activated Receptor Gamma. Nagatomo A., Nishida N., Matsuura Y.and Shibata N. Prev Nutr Food Sci, Vol.18(2), p85-91, Jun 2013.

38.

Glucose Use in Fasted Rats Under Sevoflurane Anesthesia and Propofol Anesthesia. K.Sato, T.Kitamura, G.Kawamura, Y.Mori, R.Sato, Y.Araki, Y.Yamada. Anesth Analg, Jun 2013.

39.

Supplementing Obese Zucker Rats with Niacin Induces the Transition of Glycolytic to Oxidative Skeletal Muscle Fibers. Ringseis R., Rosenbaum S., Gessner D.K., Herges L., Kubens J.F., Mooren F-C., Kruger K. and Eder K. J.Nutr, Vol.143(2), p125-131, Feb 2013.

40.

Quercetin intake during lactation modulates the AMP-activated protein kinase pathway in the livers of adult male rat offspring programmed by maternal protein restriction. Sato S., Mukai Y., Saito T. The Journal of Nutritional Biochemistry, Vol.24(1), p118-123, Jan 2013.

41.

Azuki bean polyphenols intake during lactation upregulate AMPK in male rat offspring exposed to fetal malnutrition. Mukai Y,Sun Y, Sato S. Nutrition, Vol.29(1), p291-297, Jan 2013.

42.

Effect of Angiotensin II Type 2 Receptor-Interacting Protein on Adipose Tissue Function via Modulation of Macrophage Polarization. F.Jing, M.Mogi, L.-J.Min, K.Ohshima, H.Nakaoka, K.Tsukuda, X.Wang, J.Iwanami, M.Horiuchi. PLOS one, 2013.

43.

Prevention mechanisms of glucose intolerance and obesity by cacao liquor procyanidin extract in high-fat diet-fed C57BL/6 mice. Y. Yamashita., M. Okabe., M. Natsume., H. Ashida. Archives of Biochemistry and Biophysics, Mar 2012.

44.

High-fat diet-induced reduction of peroxisome proliferator-activated receptor-γ coactivator-1α messenger RNA levels and oxidative capacity in the soleus muscle of rats with metabolic syndrome. F. Nagatomo., H. Fujino., H. Kondo., I. Takeda., K. Tsuda., A. Ishihara. Nutrition Research, Vol.32(2), p144-151, Feb 2012.

45.

Oral Ingestion of Aloe vera Phytosterols Alters Hepatic Gene Expression Profiles and Ameliorates Obesity-Associated Metabolic Disorders in Zucker Diabetic Fatty Rats. E. Misawa., M. Tanaka., K. Nomaguchi., K. Nabeshima., M. Yamada., T. Toida., and K. Iwatsuki. J. Agric. Food Chem., Vol.60 (11), p2799-2806, 2012.

46.

The effects of running exercise on oxidative capacity and PGC-1α mRNA levels in the soleus muscle of rats with metabolic syndrome. F. Nagatomo., H. Fujino., H. Kondo., M. Kouzaki., N. Gu., I. Takeda., K. Tsuda., and A. Ishihara. The Journal of Physiological Sciences, Vol. 62, p105-114, Nov 2012.

47.

Roles of Interleukin 17 in Angiotensin II Type 1 Receptor-Mediated Insulin Resistance. K. Ohshima., M. Mogi., F. Jing., J. Iwanami., K. Tsukuda., L-J. Min., J. Higaki., M. Horiuchi. American Heart Association. Hypertension. Vol.59, p493-499, 2012.

48.

A Comparative Study of Gastric Banding and Sleeve Gastrectomy in an Obese Diabetic Rat Model. T. Masuda., M. Ohta., T. Hirashita., Y. Kawano., H. Egucji., K. Yada., Y. Iwashita., S. Kitano. Obesity Surgery, Aug 2011.

49.

Anti-Diabetic Effects of a Kaempferol Glycoside-Rich Fraction from Unripe Soybean (Edamame,Glycine max L.Merrill.’Jindai’) Leaves on KK-Ay Mice. Y. Zang., H. Sato., K. Igarashi. Biosci.Biotechnol.Biochem, Vol.75(9), p1677-1684, 2011.

50.

Effects of visceral fat resection and gastric banding in an obese diabetic rat model. T. Hirashita., M. Ohta., Y. Endo., T. Masuda., Y. Iwashita., S. Kitano. Surgery, Vol.151(1), p6-12, 2012.

51.

Preventive Effects of Curcumin on the Development of Azoxymethane-Induced Colonic Preneoplastic Lesions in Male C57BL/KsJ-db/db Obese Mice. M. Kubota., M. Shimizu., H. Sakai., Y. Yasuda., D. Terakura., A. Baba., T. Ohno., H. Tsurumi., T. Tanaka., H. Moriwaki. Nutrition and Cancer, Vol.64(1), 2012.

52.

Preventive Effects of Curcumin on the Development of Azoxymethane-Induced Colonic Preneoplastic Lesions in Male C57BL/KsJ-db/db Obese Mice. M. Kubota., M. Shimizu., H. Sakai., Y. Yasuda., D. Terakura., A. Baba., T. Ohno., H. Tsurumi., T. Tanaka., H. Moriwaki. Nutrition and Cancer, Vol.64(1), 2012.

53.

Antihyperlipidemic and Body Fat-Lowering Effects of Silk Proteins with Different Fibroin/Sericin Compositions in Mice Fed with High Fat Diet. Chung-Won Seo,In Chul Um,Catherine W.Rico,and Mi Young Kang. J.Agric.Food Chem. Vol.59, p4192-4197, 2011.

54.

Trehalose prevents adipocyte hypertrophy and mitigates insulin resistance. C,Arai.,N,Arai.,A,Mizote.,K,Kohno.,K,Iwaki.,T,Hanaya.,S,Arai.,S,Ushio.,S,Fukuda. Nutrition Research, Vol.30(12), p840-848, 2010.

55.

Combined Effects of Short-term Calorie Restriction and Exercise on Insulin Action in Normal Rats. H,Y,Jiang.,T,Koike.,P,Li.,Z,H,Wang.,Y,Kawata.,Y,Oshida. Horm Metab Res,  Vol.42(13), p950-954, 2010.

56.

Dietary Hesperidin Exerts Hypoglycemic and Hypolipidemic Effects in Streptozocin-Induce Marginal Type 1 Diabetic Rats. Akiyama,S., Katsumata,S., Suzuki,K., Ishimi,Y.,Wu,J., and Uehara,M. J Clin Biochem Nutr.January, Vol.46(1), p87-92, 2010.

57.

Hypoglycemic and Hypolipidemic Effects of Hesperidin and Cyclodextrin-Clathrated Hesperetin in Goto-Kakizaki Rats with Type 2 Diabetes. Akiyama,S., Katsumata,S., Suzuki,K., Nakayama,Y., Ishimi,Y. and Uehara,M. Bioscience,Biotechnology,and Biochemistry. Vol.73, No.12, p2779-2782, 2009.