High purity exo-1,3-β-D-Glucanase Trichoderma sp. + β-Glucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.58 (exo-1,3-β-Glucanase)
CAZy Family: GH55
CAS: 9073-49-8

glucan 1,3-beta-glucosidase; 3-beta-D-glucan glucohydrolase

EC 3.2.1.21 (β-Glucosidase)
CAZy Family: GH3
CAS: 9001-22-3

beta-glucosidase

exo-1,3-β-D-Glucanase: Recombinant. From Trichoderma sp.
β-Glucosidase: From Aspergillus niger.
In 3.2 M ammonium sulphate.

Activity/Concentration: exo-1,3-β-Glucanase (100 U/mL) / β-Glucosidase (20 U/mL).

Stability: > 2 years at 4^oC.

DESCRIPTION

exo-1,3-β-D-Glucanase + β-Glucosidase

EC 3.2.1.58 (exo-1,3-β-D-Glucanase)
CAZy Family: GH55
CAS: 9073-49-8

EC 3.2.1.21 (β-Glucosidase)
CAZY Family: GH3
CAS: 9001-22-3

Synonyms
(exo-1,3-β-D-Glucanase): glucan 1,3-beta-glucosidase; 3-beta-D-glucan glucohydrolase (β-Glucosidase): beta-glucosidase

Form:
In 3.2 M ammonium sulphate.

Stability:
> 2 years at 4^oC.

Activity:
exo-1,3-β-Glucanase: 100 U/mL 
β-Glucosidase: 20 U/mL

Unit definition:
One Unit of exo-1,3-β-glucanase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from laminarin (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40^oC.

One Unit of β-glucosidase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol per minute from 4-nitrophenyl-β-D-glucpyranoside in sodium acetate buffer (100 mM), pH 4.0 at 40^oC.

Specificity:
exo-1,3-β-glucanase: Successive hydrolysis of β-D-glucose units from the non-reducing ends of (1,3)-β-D-glucans, releasing α-glucose.

β-glucosidase: Hydrolysis of terminal, non-reducing β-D-glucosyl residues with release of β-D-glucose.

Applications:
For use in the determination of (1,3)(1,4) β-glucan.