分析物意义: 食品中常见的增甜剂有阿斯巴甜、D-甘露醇、D-山梨醇和木糖醇
Megazyme检测试剂盒优点:K-MANOL-方法新颖/只有试剂盒可用
The D-Mannitol/L-Arabitol test kit is suitable for the rapid and specific measurement and analysis of D-mannitol and L-arabitol in food, beverages and other materials.
Suitable for manual, auto-analyser and microplate formats.
Data calculators are located in the Technical Resources tab.
UV-method for the determination of D-Mannitol and L-Arabitol
in foodstuffs and other materials
Principle:
(mannitol dehydrogenase)
(1) D-Mannitol + NAD+ → D-fructose + NADH + H+
(mannitol dehydrogenase)
(2) L-Arabitol + NAD+ → L-xylulose + NADH + H+
Kit size: 60 assays (manual) / 600 (microplate)
/ 580 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 6 min
Detection limit: 0.50 mg/L
Application examples:
Wine, chewing gum, dietetic foods, candies, cosmetics, pharmaceuticals
and other materials (e.g. biological cultures, samples, etc.)
Method recognition: Novel method
Advantages
- Novel product (only enzymatic kit available)
- Very cost effective
- All reagents stable for > 2 years after preparation
- Simple format
- Rapid reaction
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Extended cofactors stability
- Suitable for manual, microplate and auto-analyser formats