A rapid, simple, reliable and accurate method for the measurement and analysis of total sulfite (sulphite) and free sulfite in wine, beverages, foodstuffs and other materials. Supplied as a “ready to use” liquid stable formulation that is suitable for manual, auto-analyser and microplate formats.
Suitable for manual, auto-analyser and microplate formats.
Colourimetric methods for the determination of Total and Free
Sulfite in wine, fruit juice, foodstuffs and other materials
Principle:
The Total Sulfite assay is based on the reaction principle
between thiol groups and Ellman’s reagent
The Free Sulfite assay is based on the reaction principle of
SO2, fuchsin and aldehyde binding
Kit size: 40 assays (manual) / 400 (microplate)
/ 400 (auto-analyser)
Method: Total sulfite: Spectrophotometric at 405 nm
Free sulfite: Spectrophotometric at 575 nm
Total assay time: Total sulfite: ~ 6 min
Free sulfite: ~ 9 min
Detection limit: Total sulfite: ~ 5 mg/L
Free sulfite: ~ 2 mg/L
Application examples:
Wine, fruit juice, seafood, food stuffs and other materials
Method recognition:
Validated for red and white wines at the Bundesamt für Weinbau, Austria.
Used widely in the wine industry
Advantages
- ”Ready to use" liquid stable Formulation
- Very competitive price (cost per test)
- All reagents stable for > 18 months
- Very rapid reaction
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual, microplate and auto-analyser formats