The L-Glutamine/Ammonia (Rapid) test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-glutamine and ammonia in culture media/supernatants and other materials.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
UV-method for the determination of L-Glutamine and Ammonia
in cell culture media, foodstuffs and other materials
Principle:
(glutaminase)
(1) L-Glutamine + H2O → L-glutamate + NH4+
(microbial glutamate dehydrogenase)
(2) NH4+ + 2-Oxoglutarate + NADPH → L-glutamate + NADP+ + H2O
Kit size: 50 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 15 min
Detection limit: 0.54 mg/L (L-glutamine)
0.06 mg/L (ammonia)
Application examples:
Cell culture media and cultures, dietary supplements, vegetables
and other materials (e.g. biological samples, etc.)
Method recognition: Novel method
Advantages
- Very rapid reaction due to use of high activity glutaminase and uninhibited glutamate dehydrogense
- All enzymes supplied as stabilised suspensions
- Only enzymatic kit available
- Very cost effective
- All reagents stable for > 2 years after preparation
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included