The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.
UV-method for the determination of L-Arabinose and D-Galactose
in hydrolysed plant products
Principle:
(galactose mutarotase)
(1) α-L-Arabinose / α-D-galactose ↔ β-L-arabinose / β-D-galactose
(β-galactose dehydrogenase)
(2) β-L-Arabinose + NAD+ → L-arabinonic acid + NADH + H+
(β-galactose dehydrogenase)
(3) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+
Kit size: 115 assays (manual) / 1150 (microplate)
/ 1150 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 1.3 mg/L
Application examples:
Analysis of hydrolysates of oligo- and polysaccharides (e.g. arabinan,
arabinoxylan, galactan, arabinogalactan), milk, dairy products, foods
containing milk (e.g. dietetic foods, bakery products, baby food,
chocolate, sweets and ice-cream), food additives (e.g. sweeteners),
cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition: Novel method