The Raffinose/Sucrose/D-Glucose test kit is for the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose, stachyose and verbascose to D-glucose, D-fructose and D-galactose.

Colourimetric method for the determination of Raffinose (also
stachyose and verbascose), Sucrose and D-Glucose in
legume seeds, plant materials, foodstuffs and feed

Principle:
(α-galactosidase)
(1) Raffinose + stachyose + verbascose + H₂O → D-galactose +
sucrose

(invertase)
(2) Sucrose + H₂O → D-glucose + D-fructose

(glucose oxidase)
(3) D-Glucose + H₂O + O₂ → D-gluconate + H₂O₂

(peroxidase)
(4) 2H₂O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H₂O

Kit size:                           120 assays
Method:                           Spectrophotometric at 510 nm
Reaction time:                 ~ 20 min
Detection limit:                100 mg/L
Application examples: 
Analysis of grain legumes and other materials containing raffinose,
stachyose and verbascose
Method recognition:         Used and accepted in food analysis

Advantages

• Very competitive price (cost per test)
  
• All reagents stable for > 2 years after preparation
  
• Simple format
  
• Rapid reaction
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included