This product (K-RHAMNOSE) supersedes the original L-Rhamnose Assay Kit (K-RHAM) which has been discontinued. K-RHAMNOSE provides a more rapid reaction (~ 5 min.) and much improved reagent stability compared to the previous kit.
The L-Rhamnose test kit is a simple, rapid, reliable and accurate method for the measurement of L-rhamnose in plant extracts, culture media/supernatants and other materials.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of L-Rhamnose in hydrolysates
of plant material, polysaccharides, culture media / supernatants
and other materials. Suitable for use with manual, microplate
and auto-analyser formats

Principle:
(L-rhamnose dehydrogenase)
(1) L-Rhamnose + NAD⁺ → L-rhamno-1,4-lactone + NADH + H⁺

Kit size:                              50 / 100 assays (manual) / 550 (microplate) 
                                            / 550 (auto-analyser)
Method:                              Spectrophotometric at 340 nm
Reaction time:                    ~ 5 min at 25°C or ~ 4 min at 37°C
Detection limit:                   ~ 1.2 mg/L
Application examples: 
Hydrolysates of plant material and polysaccharides, culture media /
supernatants and other materials
Method recognition:    Novel method

Advantages

• Very cost effective
  
• All reagents stable for > 2 years after preparation
  
• Only test kit available
  
• Simple format
  
• Rapid reaction (~ 5 min at 25°C or ~ 4 min at 37°C)
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included
  
• Suitable for manual, microplate and auto-analyser formats