The Trehalose test kit is a simple method for the rapid and reliable measurement and analysis of trehalose in foods, beverages and other materials.
Suitable for manual, auto-analyser and microplate formats.
UV-method for the determination of Trehalose and
D-Glucose in foodstuffs, beverages, and other materials
Principle:
(trehalase)
(1) Trehalose + H2O → D-glucose
(hexokinase)
(2) D-Glucose + ATP → G-6-P + ADP
(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
Kit size: 100 assays (manual) / 1000 (microplate)
/ 1100 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 8 min
Detection limit: 37.5 mg/L
Application examples:
Honey, mushrooms, bread, beer, seafood (e.g. lobster and shrimp),
fruit juices, purees and fillings, nutrition bars, surimi, dehydrated fruits
and vegetables, fruit products, white chocolate, sports drinks, dairy
products, egg products, soups and sauces, confectionery, chewing gum,
cosmetics, pharmaceuticals and other materials (e.g. biological cultures,
samples, etc.)
Method recognition: Novel method
Advantages
- Only enzymatic kit available
- Very cost effective
- All reagents stable for > 2 years after preparation
- Very rapid reaction
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual, microplate and auto-analyser formats