分析物意义: 常见食品的组分

Megazyme检测试剂盒优点: K-ACETRM 是运用AK和磷酸乙酰转移酶的新型、快速的手工检测试剂盒。试剂稳定

K-ACETAK (自动) 是一种以乙酸激酶（AK）为基础的，新型、稳定、快速的检测试剂盒，具有良好的线性。

Analyser format for the specific assay of acetic acid (acetate) in beverages and food products. On calibration, the prepared reagent is linear to > 28 micrograms of acetic acid per mL of assay solution. Content:170.5 mL of prepared reagent (e.g. 550 assays of 0.31 mL)

Analyser format UV-method for the determination of Acetic Acid
in foodstuffs, beverages and other materials

Principle:
(acetate kinase)
(1) Acetic acid + ATP → acetyl-phosphate + ADP

(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate

(D-lactate dehydrogenase)
(3) Pyruvate + NADH + H⁺ → D-lactic acid + NAD⁺

Kit size:                            550 assays 
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 10 min
Detection limit:                 10 mg/L (recommended assay format)
Application examples: 
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, 
pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products 
(and baking agents), ketchup, soy sauce, mayonnaise, dressings,
paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions),
feed and other materials (e.g. biological cultures, samples, etc.)
Method recognition:    Improved method

Advantages

• Very stable reagent when prepared for auto-analyser applications (> 7 days at 4°C)
  
• PVP incorporated to prevent tannin inhibition
  
• Linear calibration (R² ~ 0.9995) up to 30 μg/mL of acetic acid in final reaction solution
  
• Validated by the University of Wine, Suze la Rousse, France
  
• Very rapid reaction
  
• Very competitive price (cost per mL of reagent)
  
• All reagents stable for > 2 years

• Extended cofactors stability