L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.

Manual format UV-method for the determination of L-Malic Acid in foodstuffs, beverages and other materials

Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD⁺ ↔ oxaloacetate + NADH + H⁺

(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate

Kit size:                        (K-LMALR) 
                                     58 assays (manual) / 580 (microplate) or 
                                     (K-LMALL) 
                                     116 assays (manual) / 1160 (microplate)
Method:                         Spectrophotometric at 340 nm
Reaction time:               ~ 3 min
Detection limit:              0.25 mg/L
Application examples: 
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:     
Methods based on this principle have been accepted by AOAC, EEC, 
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK

Advantages

• PVP incorporated to prevent tannin inhibition
  
• Both enzymes supplied as stable suspensions
  
• Very competitive price (cost per test)
  
• All reagents stable for > 2 years after preparation
  
• Very rapid reaction (~ 3 min)
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included
  
• Extended cofactors stability
  
• Suitable for manual and microplate format