L-苹果酸[MegaQuant法]检测试剂盒 L-Malic Acid Assay Kit (MegaQuant™ Format) 货号：K-LMALMQ Megazyme中文站

发表于由

The L-Malic Acid test kit is suitable for the measurement and analysis of L-malic acid in grapes, grape juice and wine using the MegaQuant™ colorimeter (measurement at 505 nm). Suitable for white and red wines at all stages of the winemaking process.

Simple colourimetric method for the determination of L-Malic
Acid in foodstuffs, beverages and other materials

Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+

(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate

(diaphorase)
(3) INT + NADH + H+ → NAD+ + INT-formazan

Kit size:                            60 assays
Method:                            Spectrophotometric at 505 nm
Reaction time:                  ~ 6 min
Detection limit:                 0.25 mg/L
Application examples: 
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological 
cultures, samples, etc.)
Method recognition:       Novel method

Advantages

  • Novel product, patented technology
  • Highly stable reagents (at least three seasons use)
  • Very competitive price (cost per test)
  • Spectrophotometer / laboratory / expertise not required
  • Very simple procedure
  • Rapid reaction time (~6 min)
  • Standard included