Colourimetric method for the determination of myo-Inositol
in various sample matrices
Principle:
(myo-inositol dehydrogenase)
(1) myo-Inositol + NAD+ →
2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+
(diaphorase)
(2) INT + NADH + H+ → NAD+ + INT-formazan
Kit size: 50 assays
Method: Spectrophotometric at 492 nm
Reaction time: ~ 10 min
Detection limit: 0.8 mg/L
Application examples:
Animal feeds, food, baby milk formulation and other materials
Method recognition: Novel method