N-Glycopeptide Binding Protein |NEB酶试剂 New England Biolabs

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产品信息

N-Glycopeptide Binding Protein, also known as Fbs1-GYR lectin, is engineered to selectively bind and enrich a wide variety of N-glycopeptides in a complex proteomic sample to assist N-glycoprotein identification and discovery, without requiring the use of traditional lectin affinity chromatography (LAC) techniques, HILIC columns or cartridges. This engineered lectin, originating from human Fbs1 protein, exhibits improved specificity towards high mannose, complex, and hybrid N-glycans, as well as towards fucosylated and non-fucosylated, sialylated and non-sialylated, with bi-, tri- and tetra-antennary structures. N-Glycopeptide Binding Protein includes a SNAP-tag for immobilizing to create versatile workflows.

产品来源

An E. coli strain that carries human Fbs1 mutant gene.

产品类别:
Glycobiology Products,
Proteome Analysis Products

应用:
Glycobiology & Proteomics,
Glycoprotein Analysis

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • P0872S     -20    
        N-Glycopeptide Binding Protein P0872SVIAL -20 1 x 1 ml 1 mg/ml

  • 特性和用法

    贮存溶液

    20 mM Tris-HCl
    50 mM NaCl
    0.2 mM TCEP
    pH 8

    热失活

    否

    分子量

    理论上的: 45 kDa

    单位活性检测条件

    Functional Testing (N–Glycan Enrichment) – A 200 μL reaction in 20 mM Tris-HCl, 50 mM NaCl, 0.2 mM TCEP, 10% acetonitrile, pH 8.0 containing 1 nmol of fluorescently labeled sialylglycopeptide and 100 μg N–Glycopeptide Binding Protein incubated for 30 minutes at 4°C results in ≥40% enrichment as determined by UV-Vis Spectroscopy at 520 nm.

  • 优势和特性

    Features

    • Engineered lectin, a glycan-binding protein that recognizes a wide variety of N-glycoforms, for enrichment and identification of glycopeptides and glycoproteins
    • Selectively binds and enriches N-glycopeptides in a complex proteomics sample, unlike other lectin affinity chromatography (LAC) techniques, HILIC, or other lectin-based enrichment methods
    • Significantly increased glycopeptide spectrum match and glycoprotein ID and discovery
    • Versatile workflow

  • 相关产品

    相关产品

    • Trypsin-ultra™, Mass Spectrometry Grade
    • Endoproteinase LysC
    • SNAP-捕获磁珠
    • 6 孔磁性分离架

  • 参考文献

    1. Chen M. et al. (2017). An engineered high affinity Fbs1 carbohydrate binding protein for selective capture of N-glycans and N-glycopeptides. Nat Commun. May 23;8, 15487.
    2. Chen M., et al. (2023). Comparative site-specific N-glycoproteome analysis reveals aberrant N-glycosylation and gives insights into mannose-6-phosphate pathway in cancer. Commun Biol. Jan13;6(1), 48.
    3. Mersha F. et al. (2023). Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malay. Sci Rep. May 16;13(1), 7951.

操作说明、说明书 & 用法

  • 操作说明

    1. N-Glycopeptide Binding Protein Typical Reaction Protocol (NEB #P8072)

FAQs & 问题解决指南

  • FAQs

    1. How does N-Glycopeptide Binding Protein improve glycoproteomic analysis?
    2. What is the specificity of N-Glycopeptide Binding Protein?
    3. How strong does the N–Glycopeptide Binding Protein bind to a glycopeptide?
    4. Can N-Glycopeptide Binding Protein bind and enrich free glycans?
    5. Can N-Glycopeptide Binding Protein bind an intact glycoprotein?
    6. What influences the binding affinity of the N-Glycopeptide Binding Protein?