Sodium Orthovanadate (Vanadate) |NEB酶试剂 New England Biolabs

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产品信息

Sodium Orthovanadate (Vanadate, Na3VO4) is a commonly used general inhibitor for protein phosphotyrosyl phosphatases (PTPs). It is a competitive inhibitor. The inhibition by Vanadate is completely reversible upon the addition of EDTA or by dilution. Vanadate is has been activated for maximal inhibition of PTPs following the procedure described by J.A.Gordon (1).

The PTP activities are conveniently separable from the protein phosphoseryl and phosphothreonyl phosphatase (PSP) activities inhibited by Fluoride and EDTA. Routinely Vanadate is used to preserve the protein tyrosyl phosphorylation state in cells, cell lysates, and protein tyrosine kinase assays (1, 2). 

产品类别:
Protein Kinases Products,
Protein Phosphatases Products

应用:
Protein Phosphatases and Kinases

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • P0758S     -20    
        Sodium Orthovanadate (Vanadate) P0758SVIAL -20 1 x 1 ml 100 mM
    • P0758L     -20    
        Sodium Orthovanadate (Vanadate) P0758SVIAL -20 5 x 1 ml 100 mM

  • 注意事项

    1. Common buffer components such as EDTA and reducing agents may interact with Vanadate, affecting its potency (2).
    2. Storage: Sterile purified water adjusted to pH 10.0 (1,2).

  • 参考文献

    1. Gordon, J.A. (1991). Methods in Enzymology. 201, 477-482.
    2. Huyer, G. et al. (1997). J. Biol. Chem.. 272, 843-851.